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Goat Anti-Human IgG (AF647) - Secondary antibody, IgG

1 Citations COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • Host species: Goat
  • Reactive species: Human
  • Isotype: Goat IgG
  • Conjugation: AF647
In stock
Item Number
Ab181963
Grouped product items
SKU Size
Availability
 Price Qty
Ab181963-10μg
10μg
≥10
$19.90
Ab181963-100μg
100μg
≥10
$39.90
Ab181963-1mg
1mg
4
$99.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

pAb; Goat Anti-Human IgG H&L Antibody (AF647); IF, Flow

View related series
AF647 (119) CLARITY tissue clearing technology (64) Flow Cytometry (1158) Goat Anti-Human IgG (21) Goat Anti-Human IgG H&L (AF647) (1)

Basic Description

Product Name Goat Anti-Human IgG (AF647) - Secondary antibody, IgG
Synonyms Goat Anti Human IgG H&L Antibody | Anti-Human IgG H&L Antibody | Anti Human IgG H&L Antibody | Human IgG H&L Antibody | Goat Anti-Human IgG Antibody | Goat Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human IgG Antibody | Human IgG Antibody |
Specifications & Purity ExactAb™, Validated, Ex:650nm, Em:668nm, 1.0 mg/mL
Host species Goat
Conjugation AF647
Excitation(nm) Red Laser(633nm)
Emission(nm) 668nm
Ex/Em(nm) Ex:650nm;Em:668nm
Grade Em:668nm, Ex:650nm, ExactAb™, Validated
Product Description

Application
Flow: 1:200-1:2000
IF: 1:100-1:1000.

Product Properties

Isotype IgG
Purification Method antigen affinity chromatography
Concentration 1.0 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

Goat Anti-Human IgG (AF647) (Ab181963) - IF
IF analysis of EGFR (red) in A431 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Serclutamab (anti-EGFR) (Ab182867) at 1/1500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (AF647) (Ab181963) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Goat Anti-Human IgG (AF647) (Ab181963) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling CD276 (red) with Mirzotamab (anti-CD276) (Ab182952). Goat Anti-Human IgG (AF647) (Ab181963) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Goat Anti-Human IgG (AF647) (Ab181963) - Flow Cytometry
Flow Cytometry analysis of MCF-7 cells labelling IGF1R (red) with Ganitumab (anti-IGF1R) (Ab177931). Goat Anti-Human IgG (AF647) (Ab181963) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

1/200-1/2000
IF/ICC

1/100-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ24F0707767 Certificate of Analysis Jul 04, 2024 Ab181963
ZJ24F0707766 Certificate of Analysis Jul 04, 2024 Ab181963
ZJ24F0707765 Certificate of Analysis Jul 04, 2024 Ab181963
ZJ24R0600729 Certificate of Analysis Jun 21, 2024 Ab181963

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Documents & Articles

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General Protocol for Western Blotting
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Western Blotting with Fluorescently Labeled Antibodies
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Immunofluorescence Staining Protocol (Methanol permeabilization)
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Frozen section Immunofluorescence staining Tissue section Methanol permeability IF
Guidelines of Secondary Antibody for Western Blotting
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Flow Cytometry Using Methanol Permeabilization
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CLARITY Tissue Clearing Staining Technology
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Citations of This Product

How to Cite Aladdin Scientific Products?
1. Yang Liu, Qiankun Chen, Hongli Zhang, Zeyu Feng, Gang Zou, Douguo Zhang.  (2024)  Cascaded momentum-space polarization filters enabled label-free black-field microscopy for single nanoparticles analysis.  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,  121  (13): (e2321825121). 

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