A synthetic peptide derived from human Amyloid Precursor Protein (AA 756-770)
Positive Control
WB: HeLa, HEK293 and NIH/3T3 cell lysates. IHC: Human brain tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Note
This product is about to sell out and be taken off the shelves. The replacement product is Ab088722.
Product Description
Rabbit anti Human Amyloid Precursor Protein Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 IF/ICC: 1/50-1/200 Protein Function: Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Beta-amyloid 42 is a more effective reductant than beta-amyloid 40. Beta-amyloid peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts. Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain. The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purification
Shape
Liquid
Concentration
0.4 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Recombinant Amyloid Precursor Protein Antibody (Ab088726) - Western Blot All lanes: Recombinant Amyloid Precursor Protein Antibody (Ab088726) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 87 kDa Observed band size: 100-140 kDa Exposure time: 88.0 s
Recombinant Amyloid Precursor Protein Antibody (Ab088726) - IF/ICC Immunocytochemistry analysis of Amyloid Precursor Protein (green) in HeLa using Recombinant Amyloid Precursor Protein Antibody (Ab088726) and DAPI (blue).
Recombinant Amyloid Precursor Protein Antibody (Ab088726) - IHC Immunohistochemistry analysis of paraffin-embedded Human brain using Recombinant Amyloid Precursor Protein Antibody (Ab088726). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
IF/ICC
1/50 - 1/200
WB
1/500-1/1000
IHC
1/50-1/100
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
ZJ24F0606654