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Goat Anti-Mouse IgG H&L (AF647) - Secondary antibody, Goat IgG

1 Citations COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • Host species: Goat
  • Reactive species: Mouse
  • Isotype: Goat IgG
  • Conjugation: AF647
In stock
Item Number
Ab181952
Grouped product items
SKU Size
Availability
 Price Qty
Ab181952-10μg
10μg
≥10
$15.90
Ab181952-100μg
100μg
≥10
$79.90
Ab181952-1mg
1mg
3
$399.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

pAb; Goat Anti-Mouse IgG H&L Antibody (AF647); IF, Flow

View related series
AF647 (119) CLARITY tissue clearing technology (64) Flow Cytometry (1158) Goat Anti-Mouse IgG H&L (AF647) (1)

Basic Description

Product Name Goat Anti-Mouse IgG H&L (AF647) - Secondary antibody, Goat IgG
Synonyms Goat Anti-Mouse IgG H&L Antibody | Goat Anti Mouse IgG H&L Antibody | Anti-Mouse IgG H&L Antibody | Anti Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Goat Anti-Mouse IgG Antibody | Goat Anti Mouse IgG Antibody | Anti-Mouse Ig
Specifications & Purity Validated, Ex:650nm, Em:668nm, 1.0 mg/mL
Host species Goat
Immunogen Mouse IgG
Conjugation AF647
Excitation(nm) Red Laser(633nm)
Emission(nm) 668nm
Ex/Em(nm) Ex:650nm;Em:668nm
Grade Em:668nm, Ex:650nm, Validated
Product Description

Flow: 1:100-1:2,000
IF: 1:100-1:1,000

Product Properties

Isotype Goat IgG
Purification Method Antigen affinity chromatography
Concentration 1.0 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

Goat Anti-Mouse IgG H&L (AF647) (Ab181952) - IF
IF analysis of beta Tubulin (red) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab179922) at 1/1500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF647) (Ab181952) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Goat Anti-Mouse IgG H&L (AF647) (Ab181952) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (AF647) (Ab181952) at a dilution of 1/900 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

1:100-1:2000

IF/ICC

1/100-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0606610 Certificate of Analysis Jun 06, 2024 Ab181952
ZJ24F0606609 Certificate of Analysis Jun 06, 2024 Ab181952
ZJ24F0606608 Certificate of Analysis Jun 06, 2024 Ab181952

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Citations of This Product

How to Cite Aladdin Scientific Products?
1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,  61  (104936). 

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