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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab086911-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
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Ab086911-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$209.90
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Ab086911-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$329.90
|
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Ab086911-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
|
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pAb; Rabbit anti Human AASS Antibody; WB, ICC, IF; Unconjugated
| Product Name | AASS Antibody - Primary antibody, specific to AASS, Rabbit IgG |
|---|---|
| Synonyms | Alpha aminoadipic semialdehyde synthasemitochondrial antibody | LKR antibody | LKR/SDH antibody | LKRSDH antibody | LOR antibody | LORSDH antibody | Lysine ketoglutarate reductase antibody | Saccharopine dehydrogenase antibody | SDH antibody | AASS | LKRS |
| Specifications & Purity | Carrier Free, ExactAb™, Validated, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | AASS |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AASS. |
| Positive Control | WB: HUVEC cell lysate. IF/ICC: VB2 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human AASS Antibody, Polyclonal, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
AASS Antibody (Ab086911) - Western Blot
All lanes: AASS Antibody (Ab086911) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 120.0 s
AASS Antibody (Ab086911) - IF/ICC
Immunofluorescent analysis of AASS staining in VB2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with AASS Antibody (Ab086911) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
| Application | Dilution info |
|---|---|
| IF/ICC |
1/50-1/200
|
| WB |
1/500-1/1000
|