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蛋白酶K 来源于林伯氏白色念球菌

47篇引用文献 COA COA
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货号 (SKU) 包装规格 是否现货 价格 数量
P128666-25mg
 25mg 现货 Stock Image
P128666-100mg
 100mg 现货 Stock Image
P128666-500mg
 500mg 现货 Stock Image
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Amino Acid/Protein Metabolism (4559) Metabolic Enzyme/Protease (4889) Ser/Thr Protease (102)
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基本描述

产品名称 蛋白酶K 来源于林伯氏白色念球菌
英文别名 Nitrophenyl)methyl (4R,5S,6S)-6-[(1R)-1-hydroxyethyl]-4-methyl-7-oxo-3,4-diphenoxy-5-phosphonooxy-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylate | (4-nitrophenyl)methyl (4R,5S,6S)-6-[(1R)-1-hydroxyethyl]-4-methyl-7-oxo-3,4-diphenoxy-5-phosphonooxy-1-azabicyc
规格或纯度 EnzymoPure™, ≥20 units/mg dry weight
生化机理 蛋白酶 K 是一种稳定的高活性丝氨酸蛋白酶。晶体和分子结构研究的证据表明，该酶属于枯草蛋白酶家族，具有活性位催化三元组（Asp39-His69-Ser224）。它在 pH 值、缓冲盐、洗涤剂（SDS）和温度等多种环境中都很稳定。在 0.1-0.5% SDS 存在的情况下，蛋白酶 K 仍能保持活性，并能消化 DNA 制剂中的各种蛋白质和核酸酶，而不会影响分离 DNA 的完整性。
产品介绍

Proteinase K (PROK) is a serine protease with broad specificity towards aliphatic, aromatic and other hydrophobic amino acids. PROK has a molecular weight of 27,000 daltons and is Ca2+dependent. It is not inactivated by metal ion chelating agents such as EDTA, sulfhydryl reagents, PCMB, TLCK, or TPCK. It also retains activity in 0.5% SDS. It can be inhibited by PMSF or DFP.Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removal endotoxins bound to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Proteinase K (PROK) is a serine protease with broad specificity towards aliphatic, aromatic and other hydrophobic amino acids. PROK has a molecular weight of 27,000 daltons and is Ca2+dependent. It is not inactivated by metal ion chelating agents such as EDTA, sulfhydryl reagents, PCMB, TLCK, or TPCK. It also retains activity in 0.5% SDS. It can be inhibited by PMSF or DFP.
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removal endotoxins bound to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

储存与运输

浓度 ≥20 units/mg dry weight
储存温度 2-8°C储存
运输条件 冰袋运输
CAS编号和信息 39450-01-6
酶学委员会编号 3.4.21.64
单位定义 One Unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using urea denatured hemoglobin as the substrate.

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批号(Lot Number) 证书类型 货号
L2406542 分析证书 P128666
E2330830 分析证书 P128666
E2330831 分析证书 P128666
E2330792 分析证书 P128666
E2330796 分析证书 P128666
E2330916 分析证书 P128666
H2130347 分析证书 P128666
H2130345 分析证书 P128666
H2130346 分析证书 P128666
F1511080 分析证书 P128666

技术文档和文章

蛋白样品制备流程
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蛋白样品制备
Protein sample preparation process
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Protein sample preparation
CRISPR-Cas9和靶向基因组编辑
Categories: 技术文章
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核酸酶 细胞和分子生物学研究 靶向效率 脱靶突变
先进的酶分析技术
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生物制药 酶催化 酶活性 酶分析技术
Protein sample preparation process
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Protein sample preparation
Protein sample preparation process
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Protein sample preparation
用于交联染色质免疫沉淀的ChIP方案 （X-ChIP）
Categories: 实验方案（Protocols）
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电泳 交联 染色质免疫沉淀
原位杂交（ISH）实验方案
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蛋白酶 石蜡切片 冷冻切片 原位杂交 ISH RNA探针 DNA探针 地高辛标记
In Situ Hybridization (ISH) Protocol
Categories: 实验方案（Protocols）
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protease Paraffin Section Frozen section ISH In situ hybridization RNA probe DNA probe Digoxigenin labeling
In Situ Hybridization (ISH) Protocol
Categories: 实验方案（Protocols）
探索主题:
protease Paraffin Section Frozen section ISH In situ hybridization RNA probe DNA probe Digoxigenin labeling
细胞凋亡DNA片段化分析实验方案
Categories: 实验方案（Protocols）
探索主题:
凝胶电泳 核酸酶 细胞凋亡 RNase 凋亡细胞 DNA片段化 组织解离酶
双交联ChIP方案
Categories: 实验方案（Protocols）
探索主题:
抗体 核酸酶 免疫沉淀 交联 ChIP 蛋白酶K 双交联@甲醛 EGS 蛋白-DNA互作
Dual Cross-linking ChIP Protocol
Categories: 实验方案（Protocols）
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Antibody Nuclease Immunoprecipitation Cross-linking ChIP Proteinase K EGS double cross-linking@formaldehyde protein-DNA interaction
Dual Cross-linking ChIP Protocol
Categories: 实验方案（Protocols）
探索主题:
Antibody Nuclease Immunoprecipitation Cross-linking ChIP Proteinase K EGS double cross-linking@formaldehyde protein-DNA interaction
蛋白酶K与核糖核酸酶A
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蛋白酶K 核糖核酸酶A
Proteinase K and Ribonuclease A
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Proteinase K Ribonuclease A

此产品的引用文献

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引用文献

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