| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| E425359-1ml |
1ml |
现货  |
|
| 别名 | 反-环氧丁二酰基-L-亮氨酰胺基(4-胍基)丁烷 | L-反-3-羧基环氧乙烷-2-羰基)-L-亮氨酰胍基丁胺 | N-(反式-环氧丁二酰基)-L-亮氨酸-胍基丁基酰胺 |
|---|---|
| 英文别名 | SCHEMBL4740187 | BDBM50157741 | (2S,3S)-3-(N-{(S)-1-[N-(4-guanidinobutyl)carbamoyl]3-methylbutyl}carbamoyl)oxirane-2-carboxylic acid | AKOS016007415 | (2S,3S)-3-[[(2S)-1-[4-(diaminomethylideneamino)butylamino]-4-methyl-1-oxopentan-2-yl]carbamoyl]oxirane-2 |
| 规格或纯度 | 10mM in DMSO |
| 英文名称 | E-64 |
| 生化机理 | E-64是一个不可逆的，强有力的，高选择性半胱氨酸蛋白酶抑制剂。E-64不和非蛋白酶的官能硫基基团发生反应，如L-乳酸脱氢酶或肌酸激酶。 E-64像其他半胱氨酸蛋白酶抑制剂一样，如亮肽素和抗痛素，不会抑制丝氨酸蛋白酶（胰蛋白酶除外）。E-64的反式-环氧丁二酰基（活性部分）不可逆地结合在许多的半胱氨酸蛋白酶的活性巯基基团，如木瓜蛋白酶，猕猴桃酶(actinidase)，组织蛋白酶B,H，和L。 E-64是一种体内研究非常有用的半胱氨酸蛋白酶抑制剂，因为它有专一性的抑制特点，它可渗透到细胞和组织中，并具有低毒性。不可逆的，有效的和选择性的半胱氨酸蛋白酶抑制剂（组织蛋白酶K，S和L在体外的IC 50值分别为1.4、4.1和2.5 nM）。不抑制丝氨酸蛋白酶（胰蛋白酶除外）。 |
| 储存温度 | 避光,-80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 产品介绍 |
E-64是一种亲和纯化半胱氨酸蛋白酶的有效配体。当耦合到硫基化的亲和基质时，结合不再是不可逆的，但保留特异性 E-64 is a cysteine protease inhibitor that was isolated from the mold Aspergillus japonicus TPR-64. E-64 is also known as N-[N-(L-3-trans-carboxyoxiran-2-carbonyl)-L-leucyl]-agmatine. E-64 effectively inhibits various cysteine proteases, in particular: • cathepsin K • cathepsin L • cathepsin S E-64 also acts against other enzymes, such as: • calpain • cathepsin B • cathepsin H • papain protease inhibitor ，E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retained E-64 is a cysteine protease inhibitor that was isolated from the mold Aspergillus japonicus TPR-64. E-64 is also known as N-[N-(L-3-trans-carboxyoxiran-2-carbonyl)-L-leucyl]-agmatine. E-64 effectively inhibits various cysteine proteases, in particular: • cathepsin K • cathepsin L • cathepsin S E-64 also acts against other enzymes, such as: • calpain • cathepsin B • cathepsin H • papain application： It is used as a broad spectrum cysteine proteinase and calpain activation inhibitor. Additionally, Prevotella intermediate studies report that E-64 blocks the breakdown of methaemoglobin and the oxidation of oxyhaemoglobin by InpA. E-64 has also been used to study excystation in Giardia lamblia. E-64 is an inhibitor of Calpain, cathepsin K and cathepsin S. E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retained |
| 活性类型 | Relation | Activity value | Units | Action Type | 期刊 | PubMed Id | doi | Assay Aladdin ID |
|---|
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
| 分子类型 | 小分子 |
|---|---|
| IIUPAC Name | (2S,3S)-3-[[(2S)-1-[4-(diaminomethylideneamino)butylamino]-4-methyl-1-oxopentan-2-yl]carbamoyl]oxirane-2-carboxylic acid |
| INCHI | 1S/C15H27N5O5/c1-8(2)7-9(20-13(22)10-11(25-10)14(23)24)12(21)18-5-3-4-6-19-15(16)17/h8-11H,3-7H2,1-2H3,(H,18,21)(H,20,22)(H,23,24)(H4,16,17,19)/t9-,10-,11-/m0/s1 |
| InChi Key | LTLYEAJONXGNFG-DCAQKATOSA-N |
| Smiles | CC(C)C[C@H](NC(=O)[C@@H]1O[C@H]1C(O)=O)C(=O)NCCCCNC(N)=N |
| Isomeric SMILES | CC(C)C[C@@H](C(=O)NCCCCN=C(N)N)NC(=O)[C@@H]1[C@H](O1)C(=O)O |
| 分子量 | 357.41 |
| Beilstein号 | 1405664 |
| 敏感性 | 对光线敏感；对湿度敏感 |
|---|---|
| 熔点 | >170°C |
| 分子量 | 357.410 g/mol |
| XLogP3 | -0.900 |
| 氢键供体数Hydrogen Bond Donor Count | 5 |
| 氢键受体数Hydrogen Bond Acceptor Count | 6 |
| 可旋转键计数Rotatable Bond Count | 11 |
| 精确质量Exact Mass | 357.201 Da |
| 单同位素质量Monoisotopic Mass | 357.201 Da |
| 拓扑极表面积Topological Polar Surface Area | 172.000 Ų |
| 重原子数Heavy Atom Count | 25 |
| 形式电荷Formal Charge | 0 |
| 复杂度Complexity | 518.000 |
| 同位素原子数Isotope Atom Count | 0 |
| 定义的原子立体中心计数Defined Atom Stereocenter Count | 3 |
| 未定义的原子立体中心计数Undefined Atom Stereocenter Count | 0 |
| 定义的键立体中心计数Defined Bond Stereocenter Count | 0 |
| 未定义的键立体中心计数Undefined Bond Stereocenter Count | 0 |
| 所有立体化学键的总数The total count of all stereochemical bonds | 0 |
| 共价键合单元计数Covalently-Bonded Unit Count | 1 |
| 象形图 | GHS08 |
|---|---|
| 信号词 | 警告 |
| WGK Germany | 3 |
| RTECS | RR0390000 |
| 个人防护装备 | Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter |
| 1. Limei Cai, Xiaoming Liu, Zhiqiang Tian, J.P. Michaud, Zhongjian Shen, Zhen Li, Songdou Zhang, Xiaoxia Liu. (2022) Safety of Bacillus thuringiensis Cry1Ah and Vip3Aa toxins for the predatory stink bug Arma custos (Hemiptera: Pentatomidae). SCIENCE OF THE TOTAL ENVIRONMENT, 851 (158120). [PMID:35987246] [10.1016/j.scitotenv.2022.158120] |
| 2. Yunhua Zhang, Jinyun Ji, Xiao Zhao, Ruoheng Jin, Jianhong Li, Hu Wan. (2017) Expression, purification and enzymatic properties of a β-N-acetylhexosaminidase from brown planthopper, Nilaparvata lugens. JOURNAL OF ASIA-PACIFIC ENTOMOLOGY, 20 (1340). [10.1016/j.aspen.2017.10.005] |
| 3. Jiandong Shen, Bijiang Zhong, Wenshui Xia, Yanshun Xu. (2024) Action of structural proteins in textural deterioration of grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage. INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 59 (4): (2659-2666). [10.1111/ijfs.17010] |
首页
400-620-6333
3篇引用文献 
