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考马斯亮蓝R250 (CBB R-250)

56篇引用文献 COA COA
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货号 (SKU) 包装规格 是否现货 价格 数量
B105005-5g
 5g 现货 Stock Image
B105005-25g
 25g 现货 Stock Image
B105005-100g
 100g 现货 Stock Image
大包装询价 收藏夹 比较  SDS下载  DATASHEET  质量标准
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Fluorescent Dye (370) 蛋白质染色及染色试剂 (1) 酸性染料 (56)
main product photo

基本描述

别名 考马斯亮兰R250 | 考马斯亮蓝R | 酸性蓝83 | 亮吲哚蓝蛋白 6B | 酸性艳蓝6B | 考马斯亮蓝R-250 | 亮蓝 R
英文别名 Acid Cyanine 6B | Acid Blue 83 | Coomassie blue R-250 | Alizarin Rubinol 5G | Coomassie Brilliant Blue R-250 | Solar Cyanine 6B | CBB R-250
规格或纯度 用于电泳, ≥90%
英文名称 Brilliant Blue R
生化机理 SDS-PAGE中使用的三苯甲烷染料用于蛋白质分析。染料分子与蛋白质结合形成蛋白质-染料复合物。即使在酸性条件下，该络合物也能稳定染料的带负电荷的阴离子形式，产生蓝色。
储存温度 干燥
运输条件 常规运输
备注 如果有可能，您尽量在使用的当天配置溶液，并在当天使用完它。但是，如果您需要预先配制储备溶液，我们建议您将溶液等份保存在-20°C的密封小瓶中。通常，它们最多可以使用一个月。在使用前和打开样品瓶之前，我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度，用法和处理的建议吗？请访问我们的常见问题（FAQ）页面以获取更多详细信息。
产品介绍

不溶于冷水，微溶于热水呈艳红光蓝色，微溶于乙醇呈艳蓝色。遇浓硫酸呈橙红色。其水溶液加氢氧化钠呈紫色。 用于检测电泳后的蛋白条带。 比萘酚蓝黑B（Naphthol Blue Black B）和溴酚（Bromophenol）灵敏5-10倍（ 能检测到胶基质上0.5 μg/cm2 蛋白质)。

application：

Brilliant Blue R is used for detection of protein bands following electrophoresis. It is a sensitive stain for protein detection in polyacrylamide gels, typically giving blue bands on a clear background with a sensitivity of 50-100 ng/band. It may be combined with other stains, such as silver stain, to distinguish different types of proteins. It displays metachromasia by appearing pink-red, rather than blue, when binding certain proteins, such as collagen and histone H1.
纯度 ≥90%

关联靶点（人）

SH-SY5Y (11521 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

名称和识别符

PubChem SID 504753768
EC号 228-060-5
分子类型 小分子
IIUPAC Name sodium;3-[[4-[[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]cyclohexa-2,5-dien-1-ylidene]methyl]-N-ethylanilino]methyl]benzenesulfonate
INCHI 1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1
InChi Key NKLPQNGYXWVELD-UHFFFAOYSA-M
Smiles CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3)C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC.[Na+]
Isomeric SMILES CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3)C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC.[Na+]
分子量 825.97
Beilstein号 5718025
Reaxy-Rn 6953757
Reaxys-RN link address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=6953757&ln=

化学和物理性质

溶解性 insoluble in H2O; ≥2.08 mg/mL in EtOH with ultrasonic; ≥3.8 mg/mL in DMSO with gentle warming and ultrasonic
熔点 174-180°C
分子量 826.000 g/mol
XLogP3
氢键供体数Hydrogen Bond Donor Count 1
氢键受体数Hydrogen Bond Acceptor Count 9
可旋转键计数Rotatable Bond Count 13
精确质量Exact Mass 825.252 Da
单同位素质量Monoisotopic Mass 825.252 Da
拓扑极表面积Topological Polar Surface Area 159.000 Ų
重原子数Heavy Atom Count 58
形式电荷Formal Charge 0
复杂度Complexity 1500.000
同位素原子数Isotope Atom Count 0
定义的原子立体中心计数Defined Atom Stereocenter Count 0
未定义的原子立体中心计数Undefined Atom Stereocenter Count 0
定义的键立体中心计数Defined Bond Stereocenter Count 0
未定义的键立体中心计数Undefined Bond Stereocenter Count 0
所有立体化学键的总数The total count of all stereochemical bonds 0
共价键合单元计数Covalently-Bonded Unit Count 2

安全和危险性（GHS）

象形图 GHS07
信号词 警告
危险声明

H302: 吞食有害

H319: 引起严重眼睛刺激

H412: 对水生生物有害并具有长期持续影响
预防措施声明

P264: 处理后要彻底洗手。

P270: 使用本产品时，请勿进食、饮水或吸烟。

P273: 避免释放到环境中。

P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。

P330: 漱口

P305+P351+P338: 如进入眼睛：用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出，取出隐形眼镜。继续冲洗。

P501: 将内容物/容器处理到。。。

P264+P265: 处理后彻底洗手[和…]。不要触摸眼睛。

P301+P317: 如果被吞咽：请寻求医疗帮助。

P337+P317: 如果眼睛刺激持续：寻求医疗帮助。
WGK Germany 3
个人防护装备 Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

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批号(Lot Number) 证书类型 货号
C2512358 分析证书 B105005
C2512367 分析证书 B105005
C2512368 分析证书 B105005
K2413233 分析证书 B105005
K2413235 分析证书 B105005
F2414363 分析证书 B105005
F2414364 分析证书 B105005
A2305258 分析证书 B105005
A2305259 分析证书 B105005
A2305260 分析证书 B105005
A2305261 分析证书 B105005
A2305262 分析证书 B105005
A2305323 分析证书 B105005
F2214451 分析证书 B105005
F2214452 分析证书 B105005
F2214453 分析证书 B105005

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技术文档和文章

阿拉丁@生物实验常用缓冲液配方指南
Categories: 技术文章
探索主题:
生物缓冲液 电泳 生化试剂 抗生素 Western blot 染色剂和染料
Aladdin@Guidelines for the formulation of commonly used buffers for biological experiments
Categories: 技术文章
探索主题:
Western blot Biochemical reagents Antibiotics Stains and dyes Electrophoresis Biological buffers
Aladdin@Guidelines for the formulation of commonly used buffers for biological experiments
Categories: 技术文章
探索主题:
Western blot Biochemical reagents Antibiotics Stains and dyes Electrophoresis Biological buffers
Aladdin@Guidelines for the formulation of commonly used buffers for biological experiments
Categories: 技术文章
探索主题:
Western blot Biochemical reagents Antibiotics Stains and dyes Electrophoresis Biological buffers
Aladdin@Guidelines for the formulation of commonly used buffers for biological experiments
Categories: 技术文章
探索主题:
Western blot Biochemical reagents Antibiotics Stains and dyes Electrophoresis Biological buffers
GST融合蛋白沉降技术实验方案
Categories: 实验方案（Protocols）
探索主题:
蛋白质电泳和蛋白质印迹 GST蛋白沉降技术 蛋白-蛋白互作
GST Pull-Down Protocol
Categories: 实验方案（Protocols）
探索主题:
Protein Electrophoresis & Western Blotting GST Pull-Down Protein-protein interactions
GST Pull-Down Protocol
Categories: 实验方案（Protocols）
探索主题:
Protein Electrophoresis & Western Blotting GST Pull-Down Protein-protein interactions
蛋白质印迹通用实验方案
Categories: 实验方案（Protocols）
探索主题:
免疫印迹 生物缓冲液 蛋白质印迹 WB 蛋白质电泳和蛋白质印迹 偶联二抗 HRP
General Protocol for Western Blotting
Categories: 实验方案（Protocols）
探索主题:
Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers HRP Immunoblotting Conjugated Secondary Antibodies
General Protocol for Western Blotting
Categories: 实验方案（Protocols）
探索主题:
Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers HRP Immunoblotting Conjugated Secondary Antibodies
General Protocol for Western Blotting
Categories: 实验方案（Protocols）
探索主题:
Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers HRP Immunoblotting Conjugated Secondary Antibodies
General Protocol for Western Blotting
Categories: 实验方案（Protocols）
探索主题:
Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers HRP Immunoblotting Conjugated Secondary Antibodies
General Protocol for Western Blotting
Categories: 实验方案（Protocols）
探索主题:
Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers HRP Immunoblotting Conjugated Secondary Antibodies
蛋白质印迹的裂解物制备法
Categories: 实验方案（Protocols）
探索主题:
SDS 蛋白质印迹 细胞裂解 组织解离 蛋白质提取 RIPA缓冲液
Methods of Lysate Preparation for Western Blotting
Categories: 实验方案（Protocols）
探索主题:
Western blotting SDS Cell lysis Tissue dissociation Protein extraction RIPA buffer
General Protocol for Western Blotting
Categories: 实验方案（Protocols）
探索主题:
Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers HRP Immunoblotting Conjugated Secondary Antibodies
Methods of Lysate Preparation for Western Blotting
Categories: 实验方案（Protocols）
探索主题:
Western blotting SDS Cell lysis Tissue dissociation Protein extraction RIPA buffer
荧光标记抗体的蛋白质印迹法
Categories: 实验方案（Protocols）
探索主题:
免疫印迹 生物缓冲液 蛋白质电泳和蛋白质印迹 荧光标记抗体 转膜
Western Blotting with Fluorescently Labeled Antibodies
Categories: 实验方案（Protocols）
探索主题:
Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Fluorescently Labeled Antibodies Membrane Transfer
蓝色天然聚丙烯酰胺凝胶电泳实验方案 （BN-PAGE）
Categories: 实验方案（Protocols）
探索主题:
蛋白质印迹 天然聚丙烯凝胶电泳 非变性凝胶电泳 BN-PAGE 考马斯亮蓝 蛋白质复合物 辣根过氧化物酶 碱性磷酸酶
BN-PAGE Protocol
Categories: 实验方案（Protocols）
探索主题:
Western blotting BN-PAGE Natural polypropylene gel electrophoresis Non-denaturating gel electrophoresis Coomasil brilliant blue Protein complex Horseradish peroxidase Alkaline phosphatase
BN-PAGE Protocol
Categories: 实验方案（Protocols）
探索主题:
Western blotting BN-PAGE Natural polypropylene gel electrophoresis Non-denaturating gel electrophoresis Coomasil brilliant blue Protein complex Horseradish peroxidase Alkaline phosphatase
Western Blotting with Fluorescently Labeled Antibodies
Categories: 实验方案（Protocols）
探索主题:
Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Fluorescently Labeled Antibodies Membrane Transfer
General Protocol for Western Blotting
Categories: 实验方案（Protocols）
探索主题:
Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers HRP Immunoblotting Conjugated Secondary Antibodies
适用于电泳
Categories: 阿拉丁试剂的规格、级别和纯度
探索主题:
适用于电泳
For Electrophoresis
Categories: 阿拉丁试剂的规格、级别和纯度
探索主题:
For Electrophoresis

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此产品的引用文献

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引用文献

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3. Mengchen Zhang, Chao Xing, Jianan Huang, Peishan Li, Long Jiang, Lingfeng Liu, Jianbo Jia, Xiwang Zhang, Shanqing Zhang, Changyu Liu.  (2023)  Adaptive Gating Enhances Intelligent Membranes for Cellular Functions and Precise Separations.  ADVANCED FUNCTIONAL MATERIALS,  34  (8): (2310647).  [10.1002/adfm.202310647]
4. Qingqing Liu, Yanting Liu, He Huang, Mingming Xiong, Yunting Yang, Chutian Lin, Feng Yang, Yisha Xie, Yongjun Yuan.  (2023)  Improvement of the emulsifying properties of Zanthoxylum seed protein by ultrasonic modification.  ULTRASONICS SONOCHEMISTRY,  100  (106638).  [PMID:37826892] [10.1016/j.ultsonch.2023.106638]
5. Qing Fang, Qin Liu, Zongli Xie, Matthew R. Hill, Kaisong Zhang.  (2024)  Ultra-thin PTMSP nanocomposite membranes incorporated with functionalized MoS2 nanosheets for pharmaceutical concentration and solvent recovery.  SEPARATION AND PURIFICATION TECHNOLOGY,  330  (125296).  [10.1016/j.seppur.2023.125296]
6. Xueping Liu, Qian Huang, Peng Pan, Mengqi Fang, Yadong Zhang, Shanlong Yang, Mingzhong Li, Yu Liu.  (2023)  Comparative Study of the Preparation of High-Molecular-Weight Fibroin by Degumming Silk with Several Neutral Proteases.  Polymers,  15  (16): (3383).  [PMID:37631440] [10.3390/polym15163383]
7. Caixia Li, Wenyue Li, Xia Zhang, Guidan Wang, Xuebo Liu, Yutang Wang, Lijun Sun.  (2024)  The changed structures of Cyperus esculentus protein decide its modified physicochemical characters: Effects of ball-milling, high pressure homogenization and cold plasma treatments on structural and functional properties of the protein.  FOOD CHEMISTRY,  430  (137042).  [PMID:37527578] [10.1016/j.foodchem.2023.137042]
8. Mengmeng Wang, Ying Wang, Peng Pan, Xueping Liu, Wenjing Zhang, Cheng Hu, Mingzhong Li.  (2023)  A high molecular weight silk fibroin scaffold that resists degradation and promotes cell proliferation.  BIOPOLYMERS,  114  (7): (e23554).  [PMID:37232459] [10.1002/bip.23554]
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19. Minmin Zou, Hexin Zhu, Ming Dong, Tian Zhao.  (2022)  Template Method for Synthesizing Hierarchically Porous MIL-101(Cr) for Efficient Removal of Large Molecular Dye.  Materials,  15  (16): (5763).  [PMID:36013899] [10.3390/ma15165763]
20. Nan Hu, Lin Chen, Yuze Zhang, Yue Li, Huizhen Li, Zhijun Zhang.  (2022)  BS12-modified CaCO3 nanoparticle for enhancing flotation of perilla protein from its wastewater.  POWDER TECHNOLOGY,  407  (117617).  [10.1016/j.powtec.2022.117617]
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23. Wenhui Zhang, Haiming Song, Lijing Zhu, Gang Wang, Zhixiang Zeng, Xiaocheng Li.  (2022)  High flux and high selectivity thin-film composite membranes based on ultrathin polyethylene porous substrates for continuous removal of anionic dyes.  Journal of Environmental Chemical Engineering,  10  (107202).  [10.1016/j.jece.2022.107202]
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