基本信息

产品名称	Mouse FcR Blocking Reagent
别名	Fc受体阻断剂 \| Fc受体封闭剂 \| FcR阻断剂 \| 小鼠Fc受体封闭剂 \| FcγIII受体 \| FcγII受体 \| 白细胞分化抗原16 \| 白细胞分化抗原32 \| CD16/32抗体 \| Fc受体阻断剂 \| Fc受体封闭剂 \| FcR阻断剂 \| 小鼠Fc受体封闭剂
英文别名	Fc block \| Fc Receptor Blocker \| FcR Blocking Reagent, mouse \| Mouse Fc blocker \| Fc receptor blocking antibody \| Mouse Fc Receptor Blocking Solution \| Fc Receptor Binding Inhibitor Antibody \| anti-Mouse Fc Block Monoclonal Antibody \| Fc Receptor Blocking
规格或纯度	重组, 无叠氮钠, 已验证, 高性能, 无动物源, ExactAb™, ≥95%(SDS-PAGE), 0.2 mg/mL
生化机理	CD16: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b. Mediates neutrophil activation by IgG complexes redundantly with Fcgr4. CD32: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.
宿主种属	大鼠(Rat)
特异性	Mouse CD16/CD32
种属反应性	小鼠(Mouse)
免疫原	Mouse BALB/c Macrophage J774
阳性对照	Flow Cytometry: C57BL/6 mouse splenocytes
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	lambda
SDS-PAGE	150 kDa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.2 mg/mL
储存温度	2-8°C储存,禁止冷冻
运输条件	冰袋运输
稳定性与储存	Store at 4°C.
分子类型	生物试剂/缓冲液

关联靶点（人）

FCGR2A Tbio 低亲和力免疫球蛋白γFc区受体II-a(Low affinity immunoglobulin gamma Fc region receptor II-a) (0 活性数据)

点击查看靶蛋白信息： FCGR2A

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

FCGR3A Tclin 低亲和力免疫球蛋白γFc区受体III-A(Low affinity immunoglobulin gamma Fc region receptor III-A) (0 活性数据)

点击查看靶蛋白信息： FCGR3A

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Mouse FcR Blocking Reagent (Ab219990)

Mouse FcR Blocking Reagent (Ab219990) - Flow Cytometry
Blocking of non-specific Fc Receptor-mediated fluorescent antibody binding with Mouse FcR Blocking Reagent. C57BL/6 splenocytes were stained with Rat IgG (PE) (Ab180312) after being blocked with Mouse FcR Blocking Reagent (Ab219990) (right panel) compared to an no blocking control (left panel). Left panel - No blocking control, cells were stained with Rat IgG (PE) (Ab180312) without any blocking reagent.

Flow Cytometry: Mouse FcR Blocking Reagent (Ab219990)

Mouse FcR Blocking Reagent (Ab219990) - Flow Cytometry
Blocking of non-specific Fc Receptor-mediated fluorescent antibody binding with Mouse FcR Blocking Reagent. C57BL/6 splenocytes were stained with Rat IgG (PE) (Ab180312) after being blocked with Mouse FcR Blocking Reagent (Ab219990) (red) or 5% mouse serum (green). Blue - No blocking control, cells were stained with Rat IgG (PE) (Ab180312) without any blocking reagent; Black - Unlabelled control, cells without incubation with any antibody.

应用

应用名称	稀释比例
Flow Cytometry	5.0 μL/test. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with Mouse FcR blocking reagent as 5.0 μL per 106 cells in 100 µl volume for 30 minutes at 4℃ prior to immunostai