基本信息

产品名称	Recombinant CD16/CD32 Antibody
别名	FcγIII受体 \| FcγII受体 \| 白细胞分化抗原16 \| 白细胞分化抗原32 \| CD16/32抗体
英文别名	CD32 \| Fcgr2 \| Fcr-2 \| Fcr-3 \| Ly-17 \| LyM-1 \| fcRII \| FcgRII \| Fcgr2a \| Ly-m20 \| Fc[g]RII \| F630109E10Rik \| CD16 \| CD16A \| CD16-II \| FCG3 \| FCGR3 \| FCGR3A \| FCGRIII \| FcGRIIIA \| FCR-10 \| FCRIII \| FCRIIIA \| IGFR3 \| IMD20 \| Low affinity immunoglobulin gamm
规格或纯度	无动物源, 无载体, 重组, ExactAb™, 无叠氮钠, 已验证, 高性能, ≥95%(SDS-PAGE), 见COA
生化机理	CD16: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b. Mediates neutrophil activation by IgG complexes redundantly with Fcgr4. CD32: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.
宿主种属	大鼠(Rat)
特异性	Mouse CD16/CD32
种属反应性	小鼠(Mouse)
免疫原	Mouse BALB/c Macrophage J774
阳性对照	Flow Cytometry: C57BL/6 mouse splenocytes
偶联	Unconjugated
备注	此产品可替代 A412018

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	lambda
SDS-PAGE	150 kDa
纯化方法	Protein A purified
储存缓冲液	PBS, pH 7.4
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
分子类型	抗体

关联靶点（人）

FCGR2A Tbio 低亲和力免疫球蛋白γFc区受体II-a(Low affinity immunoglobulin gamma Fc region receptor II-a) (0 活性数据)

点击查看靶蛋白信息： FCGR2A

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

FCGR3A Tclin 低亲和力免疫球蛋白γFc区受体III-A(Low affinity immunoglobulin gamma Fc region receptor III-A) (0 活性数据)

点击查看靶蛋白信息： FCGR3A

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant CD16/CD32 Antibody (Ab212150)

Recombinant CD16/CD32 Antibody (Ab212150) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD16/CD32 with Recombinant CD16/CD32 Antibody (Ab212150) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: Recombinant CD16/CD32 Antibody (Ab212150)

Recombinant CD16/CD32 Antibody (Ab212150) - Flow Cytometry
Flow cytometry analysis of the expression of CD16/CD32 on mouse spleen cells and demonstration of FCγR-mediated non-specific staining.
Left: Flow cytometry analysis of CD16/CD32 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD16/CD32 Antibody (Ab212150) at 0.2 μg/mL for 1 hour at 4°C. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
Right: C57BL/6 splenocytes were stained with Rat IgG (PE) (Ab180312) blocked with purified Recombinant CD16/CD32 Antibody (red) or 5% mouse serum (green). Blue - No blocking control, cells were stained with rat IgG (PE) without any blocking reagent; Black - Unlabelled control, cells without incubation with any antibody.