基本信息

产品名称	Recombinant CD45RO Antibody (APC)
别名	CD45RO单克隆抗体 \| Anti-CD45RO抗体 \| CD45RO抗体 \| CD45RO鼠单抗
英文别名	B220 \| CD45 antigen \| CD45R \| CD45RO \| EC 3.1.3.48 \| L-CA \| LY5 \| protein tyrosine phosphatase, receptor type, C \| receptor-type tyrosine-protein phosphatase C \| T200 glycoprotein \| T200 leukocyte common antigen \| T200receptor type, c polypeptide \| B220 a
规格或纯度	ExactAb™, 已验证, 5 μL/test
生化机理	Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).(Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
宿主种属	小鼠(Mouse)
特异性	Human CD45RO
种属反应性	人(Human)
免疫原	IL-2 dependent T cell line, CA1
阳性对照	Flow Cytometry: Human peripheral blood lymphocytes.
偶联	APC
Excitation(nm)	Red Laser(633nm)
Emission(nm)	660nm
Ex/Em(nm)	Ex:650nm;Em:660nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein G purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	5 μL/test
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

PTPRC Tchem 受体型酪氨酸蛋白磷酸酶C(Receptor-type tyrosine-protein phosphatase C) (0 活性数据)

点击查看靶蛋白信息： PTPRC

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant CD45RO Antibody (APC) (Ab213953)

Recombinant CD45RO Antibody (APC) (Ab213953) - Flow Cytometry
Flow cytometry analysis of CD45RO expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45RO Antibody (APC) (Ab213953) 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (APC) (Ab180309).

Flow Cytometry: Recombinant CD45RO Antibody (APC) (Ab213953)

Recombinant CD45RO Antibody (APC) (Ab213953) - Flow Cytometry
Flow cytometry analysis of CD45RO expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45RO Antibody (APC) (Ab213953) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	5 μL/test. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.