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Recombinant S100A8 Antibody

Recombinant S100A8 Antibody

级别和纯度:

见COA

应用:

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 小鼠(Mouse)
亚型: Rabbit IgG
克隆号: C2
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab182004-10μl	10μl	现货
Ab182004-50μl	50μL	现货
Ab182004-100μl	100μL	期货
Ab182004-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: S100A8

Accession#: P05109 (4) Gene ID: 6279 (4) MRP8 (1) S100A8 (3)

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基本信息

产品名称	Recombinant S100A8 Antibody
别名	S100钙结合蛋白A8抗体 \| S100A8抗体 \| 重组S100A8抗体
英文别名	Calgranulin-A \| Calprotectin L1L subunit \| CFAG \| Cystic fibrosis antigen \| Leukocyte L1 complex light chain \| Migration inhibitory factor-related protein 8 \| MRP-8 \| p8 \| S100 calcium-binding protein A8 \| Urinary stone protein band A \| Calgranulin-A anti
规格或纯度	重组, ExactAb™, 已验证, 见COA
宿主种属	兔(Rabbit)
特异性	S100A8
种属反应性	人(Human),小鼠(Mouse)
免疫原	A synthetic peptide derived from human S100A8 (AA 50-93).
阳性对照	WB: HL-60 lysates. IHC: Human breast cancer tissue.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
克隆号	C2
Format	Whole IgG
亚型	Rabbit IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

S100A8 Tbio 蛋白质S100-A8(Protein S100-A8) (0 活性数据)

点击查看靶蛋白信息： S100A8

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: Recombinant S100A8 Antibody (Ab182004)

Recombinant S100A8 Antibody (Ab182004) - Western Blot
All lanes: Recombinant S100A8 Antibody (Ab182004) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 13 kDa
Exposure time: 29.9 s

IHC: Recombinant S100A8 Antibody (Ab182004)

Recombinant S100A8 Antibody (Ab182004) - IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Recombinant S100A8 Antibody (Ab182004).

应用

应用名称	稀释比例
WB	1/500-1/2000
IHC	1/50-1/200

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C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0319602	分析证书	Ab182004
ZJ24F0910738	分析证书	Ab182004
ZJ24F0910737	分析证书	Ab182004

技术文档和文章

高分子量蛋白质印迹法

Categories: 实验方案（Protocols）

探索主题:

免疫印迹生物缓冲液蛋白质印迹高分子量蛋白大分子蛋白蛋白质电泳 WB抗体

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

引用文献

1. Günther Bode,Aloys Lüken,Claus Kerkhoff,Johannes Roth,Stephan Ludwig,Wolfgang Nacken. (2008-09-13) Interaction between S100A8/A9 and annexin A6 is involved in the calcium-induced cell surface exposition of S100A8/A9.. The Journal of biological chemistry, 283 ((46)): (31776-31784). [PMID:18786929]
2. Rasmussen, H H HH and 5 more authors.. (1992) Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes.. Electrophoresis, [PMID:1286667]
3. Lemarchand, P P, Vaglio, M M, Mauël, J J and Markert, M M.. (1992) Translocation of a small cytosolic calcium-binding protein (MRP-8) to plasma membrane correlates with human neutrophil activation.. The Journal of biological chemistry, (25): [PMID:1326551]
4. Schäfer, T T, Sachse, G E GE and Gassen, H G HG.. (1991) The calcium-binding protein MRP-8 is produced by human pulmonary tumor cells.. Biological chemistry Hoppe-Seyler, [PMID:2039599]
5. Odink, K K and 8 more authors.. (1987) Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis.. Nature, [PMID:3313057]
6. Lagasse, E E and Clerc, R G RG.. (1988) Cloning and expression of two human genes encoding calcium-binding proteins that are regulated during myeloid differentiation.. Molecular and cellular biology, [PMID:3405210]
7. Dorin, J R JR and 5 more authors.. (1987) A clue to the basic defect in cystic fibrosis from cloning the CF antigen gene.. Nature, [PMID:3561500]
8. Umekawa, T T and Kurita, T T.. (1994) Calprotectin-like protein is related to soluble organic matrix in calcium oxalate urinary stone.. Biochemistry and molecular biology international, [PMID:7849642]
9. Miyasaki, K T KT, Bodeau, A L AL, Murthy, A R AR and Lehrer, R I RI.. (1993) In vitro antimicrobial activity of the human neutrophil cytosolic S-100 protein complex, calprotectin, against Capnocytophaga sputigena.. Journal of dental research, [PMID:8423249]
10. Marti, T T, Erttmann, K D KD and Gallin, M Y MY.. (1996) Host-parasite interaction in human onchocerciasis: identification and sequence analysis of a novel human calgranulin.. Biochemical and biophysical research communications, (16): [PMID:8619876]
11. Ishikawa, K K, Nakagawa, A A, Tanaka, I I, Suzuki, M M and Nishihira, J J.. (2000) The structure of human MRP8, a member of the S100 calcium-binding protein family, by MAD phasing at 1.9 A resolution.. Acta crystallographica. Section D, Biological crystallography, [PMID:10771424]
12. Streichert, T T and 5 more authors.. (2001) The microbial receptor CEACAM3 is linked to the calprotectin complex in granulocytes.. Biochemical and biophysical research communications, (23): [PMID:11708798]
13. Ryckman, Carle C, Vandal, Karen K, Rouleau, Pascal P, Talbot, Mariève M and Tessier, Philippe A PA.. (2003) Proinflammatory activities of S100: proteins S100A8, S100A9, and S100A8/A9 induce neutrophil chemotaxis and adhesion.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:12626582]
14. She, Yi-Min YM and 5 more authors.. (2003) Identification of metal-binding proteins in human hepatoma lines by immobilized metal affinity chromatography and mass spectrometry.. Molecular & cellular proteomics : MCP, [PMID:14534351]
15. Vogl, Thomas T and 12 more authors.. (2004) MRP8 and MRP14 control microtubule reorganization during transendothelial migration of phagocytes.. Blood, (15): [PMID:15331440]
16. Viemann, Dorothee D and 12 more authors.. (2005) Myeloid-related proteins 8 and 14 induce a specific inflammatory response in human microvascular endothelial cells.. Blood, (1): [PMID:15598812]
17. Kerkhoff, Claus C and 5 more authors.. (2005) The arachidonic acid-binding protein S100A8/A9 promotes NADPH oxidase activation by interaction with p67phox and Rac-2.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, [PMID:15642721]
18. Nakatani, Yuichi; Yamazaki, Masatoshi; Chazin, Walter J and Yui, Satoru.. (2005) Regulation of S100A8/A9 (calprotectin) binding to tumor cells by zinc ion and its implication for apoptosis-inducing activity.. Mediators of inflammation, (24): [PMID:16258195]
19. Vogl, Thomas T, Leukert, Nadja N, Barczyk, Katarzyna K, Strupat, Kerstin K and Roth, Johannes J.. (2006) Biophysical characterization of S100A8 and S100A9 in the absence and presence of bivalent cations.. Biochimica et biophysica acta, [PMID:17050004]
20. Lim, Su Yin SY and 10 more authors.. (2008) S-nitrosylated S100A8: novel anti-inflammatory properties.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:18832721]
21. Sroussi, Herve Y HY, Köhler, Gerwald A GA, Agabian, Nina N, Villines, Dana D and Palefsky, Joel M JM.. (2009) Substitution of methionine 63 or 83 in S100A9 and cysteine 42 in S100A8 abrogate the antifungal activities of S100A8/A9: potential role for oxidative regulation.. FEMS immunology and medical microbiology, [PMID:19087201]
22. Champaiboon, Chantrakorn C, Sappington, Kaia J KJ, Guenther, Brian D BD, Ross, Karen F KF and Herzberg, Mark C MC.. (2009) Calprotectin S100A9 calcium-binding loops I and II are essential for keratinocyte resistance to bacterial invasion.. The Journal of biological chemistry, (13): [PMID:19122197]
23. Perera, Chandima C, McNeil, H Patrick HP and Geczy, Carolyn L CL.. (2010) S100 Calgranulins in inflammatory arthritis.. Immunology and cell biology, [PMID:19935766]
24. Ghavami, Saeid S and 9 more authors.. (2010) S100A8/A9 induces autophagy and apoptosis via ROS-mediated cross-talk between mitochondria and lysosomes that involves BNIP3.. Cell research, [PMID:19935772]
25. Hsu, Kenneth and 7 more authors.. (2009) ANTI-INFECTIVE PROTECTIVE PROPERTIES OF S100 CALGRANULINS.. Anti-inflammatory & anti-allergy agents in medicinal chemistry, (4): [PMID:20523765]
26. and Srikrishna, Geetha G.. (2012) S100A8 and S100A9: new insights into their roles in malignancy.. Journal of innate immunity, [PMID:21912088]
27. Averill, Michelle M MM, Kerkhoff, Claus C and Bornfeldt, Karin E KE.. (2012) S100A8 and S100A9 in cardiovascular biology and disease.. Arteriosclerosis, thrombosis, and vascular biology, [PMID:22095980]
28. Atallah, Mizhir M and 6 more authors.. (2012) Constitutive neutrophil apoptosis: regulation by cell concentration via S100 A8/9 and the MEK-ERK pathway.. PloS one, [PMID:22363402]
29. Vogl, Thomas T, Gharibyan, Anna L AL and Morozova-Roche, Ludmilla A LA.. (2012) Pro-inflammatory S100A8 and S100A9 proteins: self-assembly into multifunctional native and amyloid complexes.. International journal of molecular sciences, [PMID:22489132]
30. Berthier, Sylvie S and 6 more authors.. (2012) Molecular interface of S100A8 with cytochrome b558 and NADPH oxidase activation.. PloS one, [PMID:22808130]
31. Jia, Jie J and 6 more authors.. (2014) Target-selective protein S-nitrosylation by sequence motif recognition.. Cell, (23): [PMID:25417112]

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Recombinant S100A8 Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器