货号 (SKU)

包装规格

是否现货

价格

数量

Ab179938-10μl

10μl

现货

Ab179938-50μl

50μL

现货

Ab179938-100μl

100μL

现货

Ab179938-1ml

1ml

期货

基本信息

产品名称	alpha Tubulin Mouse mAb
别名	alpha Tubulin 抗体 \| Tubulin 抗体 \| α微管蛋白小鼠单克隆抗体 \| α微管蛋白小鼠单抗
英文别名	Alpha tubulin ubiquitous antibody \| Alpha-tubulin ubiquitous antibody \| K alpha 1 antibody \| TBA1B_HUMAN antibody \| TUBA1B antibody \| Tubulin alpha 1B antibody \| Tubulin alpha 1B chain antibody \| Tubulin alpha ubiquitous antibody \| Tubulin alpha ubiquitou
规格或纯度	ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL
宿主种属	小鼠(Mouse)
特异性	alpha tubulin
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	Recombinant human alpha tubulin
阳性对照	WB: HeLa, MCF-7, Jurkat, HepG2, A549, Raji, HEK293, NIH3T3 and C6 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	AB01/5A12
Format	Whole IgG
亚型	IgG2b
轻链亚型	kappa
SDS-PAGE	185 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	PBS, pH 7.2
防腐剂	No
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

TUBA1B Tchem 微管蛋白 α-1B 链(Tubulin alpha-1B chain) (0 活性数据)

点击查看靶蛋白信息： TUBA1B

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: alpha Tubulin Mouse mAb (Ab179938)

alpha Tubulin Mouse mAb (Ab179938) - Western Blot
All lanes: alpha Tubulin Antibody (Ab179938) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 50.0 kDa
Observed band size: 50.0 kDa
Exposure time: 8.8 s

Flow Cytometry: alpha Tubulin Mouse mAb (Ab179938)

alpha Tubulin Mouse mAb (Ab179938) - Flow Cytometry
Overlay histogram showing HeLa cells stained with alpha Tubulin Mouse mAb (Ab179938) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).

ICC/IF: alpha Tubulin Mouse mAb (Ab179938)

alpha Tubulin Mouse mAb (Ab179938) - ICC/IF
IF analysis of alpha Tubulin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with alpha Tubulin Mouse mAb (Ab179938) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

ICC/IF: alpha Tubulin Mouse mAb (Ab179938)

alpha Tubulin Mouse mAb (Ab179938) - ICC/IF
IF analysis of alpha Tubulin (Green) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with alpha Tubulin Mouse mAb (Ab179938) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

ICC/IF: alpha Tubulin Mouse mAb (Ab179938)

alpha Tubulin Mouse mAb (Ab179938) - ICC/IF
IF analysis of alpha Tubulin (Red) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with alpha Tubulin Mouse mAb (Ab179938) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179006) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

应用

应用名称	稀释比例
IF/ICC	2 μg/mL
Flow Cytometry	0.032 μg/mL - 1.0 μg/mL
WB	1/1,000-1/20,000; Predicted molecular weight: 50 kDa

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找到8个结果

批号(Lot Number)	证书类型	货号
ZJ24F1114040	分析证书	Ab179938
ZJ24F1114039	分析证书	Ab179938
ZJ24F1114038	分析证书	Ab179938
ZJ24F0809941	分析证书	Ab179938
ZJ24F0202925	分析证书	Ab179938
ZJ24F0202924	分析证书	Ab179938
ZJ24F0202926	分析证书	Ab179938
ZJ24F0202923	分析证书	Ab179938

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