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Ab179880-10μl

10μl

现货

Ab179880-50μl

50μL

现货

Ab179880-100μl

100μL

期货

Ab179880-1ml

1ml

期货

基本信息

产品名称	PIP4K2A Antibody
别名	PIP4K2A 抗体
英文别名	1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha \| Diphosphoinositide kinase 2-alpha \| EC 2.7.1.149 \| Phosphatidylinositol 5-Phosphate 4-Kinase \| Phosphatidylinositol 5-phosphate 4-kinase type II alpha \| PI(5)P 4-kinase type II alpha \| PI5P4Kalpha \| PI
规格或纯度	ExactAb™, Validated, Carrier Free, 0.5 mg/mL
宿主种属	兔(Rabbit)
特异性	PIP4K2A
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	A KLH conjugated synthetic peptide from the C-terminal region of human PIP4K2A (AA 329-363)
阳性对照	WB: HeLa, Hep G2, SK-N-SH and NIH/3T3 cell lysates. IHC: Human brain tiisue. IF/ICC: HeLa cells. Flow: Hela cells.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Protein A purified +Peptide affinity purification
物理外观	Liquid
储存缓冲液	10mM PBS, 0.09% Sodium Azide, pH7.4
防腐剂	0.09% Sodium azide
浓度	0.5 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

PIP4K2A Tbio 磷脂酰肌醇 5-磷酸 4-激酶 2 型 α(Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha) (0 活性数据)

点击查看靶蛋白信息： PIP4K2A

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: PIP4K2A Antibody (Ab179880)

PIP4K2A Antibody (Ab179880) - Western Blot
All lanes: PIP4K2A Antibody (Ab179880) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 46 kDa
Observed band size: 39, 52 kDa
Exposure time: 3.5 s

IF/ICC: PIP4K2A Antibody (Ab179880)

PIP4K2A Antibody (Ab179880) - IF/ICC
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (Human Cervical epithelial adenocarcinoma cell line) cells labeling PIP4K2A with PIP4K2A Antibody (Ab179880) at 1/25 dilution, followed by AF488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. Cytoplasmic actin is detected with AF555 conjugated with Phalloidin at 1/100 dilution (red).

IHC: PIP4K2A Antibody (Ab179880)

PIP4K2A Antibody (Ab179880) - IHC
Immunohistochemical analysis of paraffin-embedded H. brain section using PIP4K2A Antibody (Ab179880) (C-term). PIP4K2A Antibody (Ab179880) was diluted at 1/25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Flow Cytometry: PIP4K2A Antibody (Ab179880)

PIP4K2A Antibody (Ab179880) - Flow Cytometry
Flow cytometric analysis of Hela cells using PIP4K2A Antibody (Ab179880)
(C-term) (green) compared to an isotype control of rabbit IgG (blue). PIP4K2A Antibody (Ab179880) was diluted at 1/25 dilution. An AF488 goat anti-rabbit lgG at 1/400 dilution was used as the secondary antibody.

应用

应用名称	稀释比例
WB	1/1000
IHC	1/100-1/500
Flow Cytometry	1/25
IF/ICC	1/25

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ24F0303494	分析证书	Ab179880
ZJ24F0303493	分析证书	Ab179880

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

免疫荧光染色实验步骤（甲醇通透法）

Categories: 实验方案（Protocols）

探索主题:

冷冻切片免疫荧光染色组织切片甲醇通透 IF

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

利用甲醇通透法进行流式细胞术

Categories: 实验方案（Protocols）

探索主题:

流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

引用文献

1. Rameh LE, Tolias KF, Duckworth BC, Cantley LC. (1997) A new pathway for synthesis of phosphatidylinositol-4,5-bisphosphate.. Nature, 390 (6656): (192-6). [PMID:9367159]
2. Divecha, N N, Truong, O O, Hsuan, J J JJ, Hinchliffe, K A KA and Irvine, R F RF.. (1995) The cloning and sequence of the C isoform of PtdIns4P 5-kinase.. The Biochemical journal, (1): [PMID:7639683]
3. Boronenkov, I V IV and Anderson, R A RA.. (1995) The sequence of phosphatidylinositol-4-phosphate 5-kinase defines a novel family of lipid kinases.. The Journal of biological chemistry, (17): [PMID:7852364]
4. Deloukas, P P and 135 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 10.. Nature, (27): [PMID:15164054]
5. Wilcox, Andrew A and Hinchliffe, Katherine A KA.. (2008) Regulation of extranuclear PtdIns5P production by phosphatidylinositol phosphate 4-kinase 2alpha.. FEBS letters, (16): [PMID:18364242]
6. Bultsma, Yvette Y, Keune, Willem-Jan WJ and Divecha, Nullin N.. (2010) PIP4Kbeta interacts with and modulates nuclear localization of the high-activity PtdIns5P-4-kinase isoform PIP4Kalpha.. The Biochemical journal, (1): [PMID:20583997]
7. Davis, Mindy I MI and 13 more authors.. (2013) A homogeneous, high-throughput assay for phosphatidylinositol 5-phosphate 4-kinase with a novel, rapid substrate preparation.. PloS one, [PMID:23326584]
8. Clarke, Jonathan H JH and Irvine, Robin F RF.. (2013) Enzyme activity of the PIP4K2A gene product polymorphism that is implicated in schizophrenia.. Psychopharmacology, [PMID:24081551]
9. Sumita, Kazutaka and 20 more authors.. (2016) The Lipid Kinase PI5P4Kβ Is an Intracellular GTP Sensor for Metabolism and Tumorigenesis.. Molecular cell, (21): [PMID:26774281]
10. Wang, Diana G and 14 more authors.. (2019) PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism.. Cell reports, (14): [PMID:31091439]

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体积（添加到小瓶中）

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所需的复溶浓度

PIP4K2A Antibody

库存信息

库存信息

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库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

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质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器

PIP4K2A Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

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