CD235a Mouse mAb (Biotin)

COA

级别和纯度:

已验证

ExactAb™

无叠氮钠

1.0 mg/mL

应用:

Flow Cytometry

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG2b
克隆号: HIR2
偶联: Biotin

有货

库存信息

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库存信息

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库存信息

关闭

货号 (SKU)	包装规格	是否现货
Ab179691-10μg	10μg	现货
Ab179691-25μg	25μg	现货
Ab179691-100μg	100μg	现货

查看相关系列

点击查看靶蛋白信息: GYPA

Accession#:P02724 (22) CD235 (5) CD235a (21) Gene ID:2993 (22) GYPA (22) 生物素 (246)

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基本信息

产品名称	CD235a Mouse mAb (Biotin)
别名	生物素标记的小鼠抗人CD235a抗体 \| CD235a 小鼠单克隆抗体(生物素) \| CD235a 小鼠单抗(生物素)
英文别名	MN sialoglycoprotein \| PAS-2 \| Sialoglycoprotein alpha \| CD235a antigen \| CD235a \| glycophorin A (includes MN blood group) \| glycophorin A (MNS blood group) \| Glycophorin A \| glycophorin Erik \| glycophorin MiI \| glycophorin MiIII \| glycophorin MiV \| glyco
规格或纯度	ExactAb™, 已验证, 无叠氮钠, 1.0 mg/mL
宿主种属	小鼠(Mouse)
特异性	GYPA
种属反应性	人(Human)
交叉反应	GYPB
免疫原	Synthetic peptide (Human, N-terminal)
阳性对照	Flow: K562
偶联	Biotin

产品属性

克隆类型	单克隆抗体
克隆号	HIR2
Format	Whole IgG
亚型	Mouse IgG2b
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	1.0 mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	未稀释的抗体溶液应保存在 2°C 至 8°C 之间，避免长时间暴露在光线下。切勿冷冻。
分子类型	抗体

关联靶点（人）

GYPA Tbio 血型糖蛋白-A(Glycophorin-A) (0 活性数据)

点击查看靶蛋白信息： GYPA

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Flow Cytometry: CD235a Mouse mAb (Biotin) (Ab179691)

CD235a Mouse mAb (Biotin) (Ab179691) - Flow Cytometry
Flow cytometry analysis of CD235a expression by K562 cells (red) compared to an isotype control (blue). Cells were stained with CD235a Mouse mAb (Biotin) (Ab179691) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	≤1.0μg/test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

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C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0303125	分析证书	Ab179691
ZJ24F0303126	分析证书	Ab179691
ZJ24F0303124	分析证书	Ab179691

技术文档和文章

天然蛋白免疫沉淀法

Categories: 实验方案（Protocols）

探索主题:

免疫沉淀蛋白质印迹天然蛋白非变性生物素偶联链霉亲和素

Immunoprecipitation Protocol for Native Proteins

Categories: 实验方案（Protocols）

探索主题:

Western blotting Immunoprecipitation Native Protein Non-denaturing Biotin Conjugation Streptavidin

Immunoprecipitation Protocol for Native Proteins

Categories: 实验方案（Protocols）

探索主题:

Western blotting Immunoprecipitation Native Protein Non-denaturing Biotin Conjugation Streptavidin

可替换产品

引用文献

1. Bruce LJ, Pan RJ, Cope DL, Uchikawa M, Gunn RB, Cherry RJ, Tanner MJ. Altered structure and anion transport properties of band 3 (AE1, SLC4A1) in human red cells lacking glycophorin A.. J Biol Chem, [PMID:14604989]
2. Tomita, M M and Marchesi, V T VT.. (1975) Amino-acid sequence and oligosaccharide attachment sites of human erythrocyte glycophorin.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:1059087]
3. Huang, C H CH, Skov, F F, Daniels, G G, Tippett, P P and Blumenfeld, O O OO.. (1992) Molecular analysis of human glycophorin MiIX gene shows a silent segment transfer and untemplated mutation resulting from gene conversion via sequence repeats.. Blood, (1): [PMID:1421409]
4. Huang, C H CH, Spruell, P P, Moulds, J J JJ and Blumenfeld, O O OO.. (1992) Molecular basis for the human erythrocyte glycophorin specifying the Miltenberger class I (MiI) phenotype.. Blood, (1): [PMID:1611092]
5. Hamid, J J and Burness, A T AT.. (1990) The mechanism of production of multiple mRNAs for human glycophorin A.. Nucleic acids research, (11): [PMID:2216775]
6. Dill, K K, Hu, S H SH, Berman, E E, Pavia, A A AA and Lacombe, J M JM.. (1990) One- and two-dimensional NMR studies of the N-terminal portion of glycophorin A at 11.7 Tesla.. Journal of protein chemistry, [PMID:2386609]
7. Kudo, S S and Fukuda, M M.. (1989) Structural organization of glycophorin A and B genes: glycophorin B gene evolved by homologous recombination at Alu repeat sequences.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2734312]
8. Tate, C G CG and Tanner, M J MJ.. (1988) Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins alpha and delta.. The Biochemical journal, (15): [PMID:3196288]
9. Rahuel, C C and 8 more authors.. (1988) Characterization of cDNA clones for human glycophorin A. Use for gene localization and for analysis of normal of glycophorin-A-deficient (Finnish type) genomic DNA.. European journal of biochemistry, (15): [PMID:3345758]
10. Siebert, P D PD and Fukuda, M M.. (1986) Isolation and characterization of human glycophorin A cDNA clones by a synthetic oligonucleotide approach: nucleotide sequence and mRNA structure.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3456608]
11. Siebert, P D PD and Fukuda, M M.. (1986) Molecular biological study of the structure and expression of human glycophorin A.. Revue francaise de transfusion et immuno-hematologie, [PMID:3809885]
12. Pasvol, G G, Wainscoat, J S JS and Weatherall, D J DJ.. (1982) Erythrocytes deficiency in glycophorin resist invasion by the malarial parasite Plasmodium falciparum.. Nature, (6): [PMID:7040988]
13. Kudo, S S, Onda, M M and Fukuda, M M.. (1994) Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals.. Journal of biochemistry, [PMID:7798177]
14. Huang, C H CH, Reid, M M, Daniels, G G and Blumenfeld, O O OO.. (1993) Alteration of splice site selection by an exon mutation in the human glycophorin A gene.. The Journal of biological chemistry, (5): [PMID:8245024]
15. Pisano, A A, Redmond, J W JW, Williams, K L KL and Gooley, A A AA.. (1993) Glycosylation sites identified by solid-phase Edman degradation: O-linked glycosylation motifs on human glycophorin A.. Glycobiology, [PMID:8286855]
16. Adams, P D PD, Engelman, D M DM and Brünger, A T AT.. (1996) Improved prediction for the structure of the dimeric transmembrane domain of glycophorin A obtained through global searching.. Proteins, [PMID:8953647]
17. MacKenzie, K R KR, Prestegard, J H JH and Engelman, D M DM.. (1997) A transmembrane helix dimer: structure and implications.. Science (New York, N.Y.), (4): [PMID:9082985]
18. Poole, J and 7 more authors.. (1999) Glycophorin A mutation Ala65 --> Pro gives rise to a novel pair of MNS alleles ENEP (MNS39) and HAG (MNS41) and altered Wrb expression: direct evidence for GPA/band 3 interaction necessary for normal Wrb expression.. Transfusion medicine (Oxford, England), [PMID:10354388]
19. Storry, J R JR, Coghlan, G G, Poole, J J, Figueroa, D D and Reid, M E ME.. (2000) The MNS blood group antigens, Vr (MNS12) and Mt(a) (MNS14), each arise from an amino acid substitution on glycophorin A.. Vox sanguinis, [PMID:10729812]
20. Young, Mark T MT and Tanner, Michael J A MJ.. (2003) Distinct regions of human glycophorin A enhance human red cell anion exchanger (band 3; AE1) transport function and surface trafficking.. The Journal of biological chemistry, (29): [PMID:12813056]
21. Yajima, Ayako A and 5 more authors.. (2008) Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane.. Microbiology and immunology, [PMID:18380804]
22. Pang, Allison J AJ and Reithmeier, Reinhart A F RA.. (2009) Interaction of anion exchanger 1 and glycophorin A in human erythroleukaemic K562 cells.. The Biochemical journal, (15): [PMID:19438409]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

CD235a Mouse mAb (Biotin)

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器