Recombinant CD3 Antibody (PE)

COA

级别和纯度:

5μL/ test

应用:

Flow Cytometry

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG2a
克隆号: OKT3
偶联: PE

有货

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab178033-10T	10T	现货
Ab178033-25T	25T	现货
Ab178033-100T	100T	现货

查看相关系列

点击查看靶蛋白信息: CD3E

Accession#:P07766 (43) CD3 (46) CD3E (62) Gene ID:916 (41) PE (196) 流式细胞术 (1163)

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基本信息

产品名称	Recombinant CD3 Antibody (PE)
别名	藻红蛋白偶联的抗CD3抗体 \| 重组CD3抗体(PE)
英文别名	CD_antigen: CD3e \| CD3 antigen, delta subunit \| CD3 \| CD3d antigen \| CD3d antigen, delta polypeptide (TiT3 complex) \| CD3d molecule, delta (CD3-TCR complex) \| CD3-DELTA \| CD3e antigen \| CD3e antigen, epsilon polypeptide (TiT3 complex) \| CD3e molecule, eps
规格或纯度	ExactAb™, 重组, 已验证, 无叠氮钠, Ex:565nm, Em:575nm, 5μL/ test
宿主种属	小鼠(Mouse)
特异性	CD3
种属反应性	人(Human)
阳性对照	Flow: Jurkat
偶联	PE
Excitation(nm)	Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)	575nm
Ex/Em(nm)	Ex:565nm;Em:575nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
亚型	Mouse IgG2a
轻链亚型	kappa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	5μL/ test
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	未稀释的抗体溶液应在 2°C 至 8°C 之间保存一年。请勿冷冻。避光保存。
分子类型	抗体

关联靶点（人）

CD3E Tclin 细胞表面糖蛋白 CD3 ε 链(T-cell surface glycoprotein CD3 epsilon chain) (0 活性数据)

点击查看靶蛋白信息： CD3E

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 活性数据)

点击查看靶蛋白信息： CD3E

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Flow Cytometry: Recombinant CD3 Antibody (PE) (Ab178033)

Recombinant CD3 Antibody (PE) (Ab178033) - Flow Cytometry
Flow cytometry analysis of CD3 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD3 Antibody (PE) (Ab178033) at 5μL/test for 1 hour at 4°C. Blue - Isotype control. Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0102299	分析证书	Ab178033
ZJ24F0102300	分析证书	Ab178033
ZJ24F0102301	分析证书	Ab178033

技术文档和文章

利用甲醇通透法进行流式细胞术

Categories: 实验方案（Protocols）

探索主题:

流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

叠氮化钠去除实验

Categories: 实验方案（Protocols）

探索主题:

重组抗体叠氮化钠去除抗体纯化葡聚糖凝胶G-25 无叠氮化钠抗体

Sodium Azide Removal Protocol

Categories: 实验方案（Protocols）

探索主题:

Recombinant antibody Sodium Azide Removal Antibody Purification Sephadex G-25 Sodium Azide-Free Antibody

可替换产品

引用文献

1. Linke R, Klein A, Seimetz D. (2010) Catumaxomab: clinical development and future directions.. MAbs, 2 (2): (129-36). [PMID:20190561]
2. Soudais C, de Villartay JP, Le Deist F, Fischer A, Lisowska-Grospierre B. (1993) Independent mutations of the human CD3-epsilon gene resulting in a T cell receptor/CD3 complex immunodeficiency.. Nat Genet, 3 (1): (77-81). [PMID:8490660]
3. de Saint Basile G, Geissmann F, Flori E, Uring-Lambert B, Soudais C, Cavazzana-Calvo M, Durandy A, Jabado N, Fischer A, Le Deist F. (2004) Severe combined immunodeficiency caused by deficiency in either the delta or the epsilon subunit of CD3.. J Clin Invest, 114 (10): (1512-7). [PMID:15546002]
4. Borroto A, Reyes-Garau D, Jiménez MA, Carrasco E, Moreno B, Martínez-Pasamar S, Cortés JR, Perona A, Abia D, Blanco S et al.. (2016) First-in-class inhibitor of the T cell receptor for the treatment of autoimmune diseases.. Sci Transl Med, 8 (370): (370ra184). [PMID:28003549]
5. Sun LL, Ellerman D, Mathieu M, Hristopoulos M, Chen X, Li Y, Yan X, Clark R, Reyes A, Stefanich E et al.. (2015) Anti-CD20/CD3 T cell-dependent bispecific antibody for the treatment of B cell malignancies.. Sci Transl Med, 7 (287): (287ra70). [PMID:25972002]
6. Lanier, L L LL, Chang, C C, Spits, H H and Phillips, J H JH.. (1992) Expression of cytoplasmic CD3 epsilon proteins in activated human adult natural killer (NK) cells and CD3 gamma, delta, epsilon complexes in fetal NK cells. Implications for the relationship of NK and T lymphocytes.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:1387664]
7. Barber, E K EK, Dasgupta, J D JD, Schlossman, S F SF, Trevillyan, J M JM and Rudd, C E CE.. (1989) The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2470098]
8. Gold, D P DP and 6 more authors.. (1986) Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex.. Nature, [PMID:3012357]
9. Salomon, Arthur R AR and 10 more authors.. (2003) Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry.. Proceedings of the National Academy of Sciences of the United States of America, (21): [PMID:12522270]
10. Brill, Laurence M LM and 5 more authors.. (2004) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.. Analytical chemistry, (15): [PMID:15144186]
11. Gimferrer, Idoia I and 8 more authors.. (2004) Relevance of CD6-mediated interactions in T cell activation and proliferation.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:15294938]
12. Arnett, Kelly L KL, Harrison, Stephen C SC and Wiley, Don C DC.. (2004) Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.. Proceedings of the National Academy of Sciences of the United States of America, (16): [PMID:15534202]
13. Gaglia, Jason and Kissler, Stephan.. (2019) Anti-CD3 Antibody for the Prevention of Type 1 Diabetes: A Story of Perseverance.. Biochemistry, (8): [PMID:31523950]
14. Clevers, H C HC, Dunlap, S S, Wileman, T E TE and Terhorst, C C.. (1988) Human CD3-epsilon gene contains three miniexons and is transcribed from a non-TATA promoter.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3267235]
15. Fütterer, K K, Wong, J J, Grucza, R A RA, Chan, A C AC and Waksman, G G.. (1998) Structural basis for Syk tyrosine kinase ubiquity in signal transduction pathways revealed by the crystal structure of its regulatory SH2 domains bound to a dually phosphorylated ITAM peptide.. Journal of molecular biology, (21): [PMID:9698567]
16. Mathur D, Root AR, Bugaj-Gaweda B, Bisulco S, Tan X, Fang W, Kearney JC, Lucas J, Guffroy M, Golas J et al.. (2020) A Novel GUCY2C-CD3 T-Cell Engaging Bispecific Construct (PF-07062119) for the Treatment of Gastrointestinal Cancers.. Clin Cancer Res, 26 (9): (2188-2202). [PMID:31996389]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Recombinant CD3 Antibody (PE)

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器