基本信息

产品名称	Recombinant CD3 Antibody
别名	白细胞分化抗原3 \| 分化簇3 \| T3复合物 \| T 细胞受体复合物的 CD3 epsilon 亚基 \| CD3e \| CD3 ε 抗体 \| Anti-CD3e抗体 \| 小鼠抗人CD3 epsilon抗体
规格或纯度	无动物源, 无载体, 重组, ExactAb™, 无叠氮钠, 已验证, 高性能, ≥95%(SDS-PAGE), 见COA
生化机理	Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx.
宿主种属	小鼠(Mouse)
特异性	Human CD3ε
种属反应性	人(Human)
免疫原	Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
阳性对照	Flow Cytometry: Human peripheral blood lymphocytes
偶联	Unconjugated

产品属性

克隆类型	重组抗体
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
储存缓冲液	PBS, pH 8.0
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C long time. Upon delivery aliquot. Avoid freeze / thaw cycle.
分子类型	抗体

关联靶点（人）

CD3E Tclin 细胞表面糖蛋白 CD3 ε 链(T-cell surface glycoprotein CD3 epsilon chain) (0 活性数据)

点击查看靶蛋白信息： CD3E

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant CD3 Antibody (Ab219596)

Recombinant CD3 Antibody (Ab219596) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD3 with Recombinant CD3 Antibody (Ab219596) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Mouse IgG (Ab170221) - Isotype Control (left panel) and detected with Alexa Fluor 488-conjugated affinipure goat anti-Mouse IgG.

Flow Cytometry: Recombinant CD3 Antibody (Ab219596)

ecombinant CD3 Antibody (Ab219596) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD3 (red) with Recombinant CD3 Antibody (Ab219596) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with Alexa Fluor 488-conjugated affinipure goat anti-Mouse IgG. Blue - Isotype control, compared with Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.