基本信息

产品名称	p53 Mouse mAb
别名	肿瘤蛋白p53 \| 肿瘤蛋白p53抗体 \| 肿瘤蛋白p53小鼠单克隆抗体 \| TP53抗体 \| p53抗体 \| 小鼠抗人肿瘤蛋白p53抗体
英文别名	p53 induced gene 11 protein antibody \| p53 induced protein antibody \| PIG 11 antibody \| PIG11 antibody \| Tumor protein p53 inducible protein 11 antibody \| Anti- p53 \| Mouse Anti-human p53 \| Monoclonal Anti- p53 antibody \| p53 Mouse Monoclonal Antibody \| p
规格或纯度	无动物源, ExactAb™, 无载体, 已验证, 无叠氮钠, 高性能, ≥95%(SDS-PAGE), 1.0 mg/mL
生化机理	Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence. Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2. However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2.
宿主种属	小鼠(Mouse)
特异性	Human p53
种属反应性	人(Human)
预测反应种属	Pan paniscus,Pan troglodytes
免疫原	Recombinant protein corresponding to Human p53
阳性对照	WB: HEK293 and A431 whole cell lysates Flow Cytometry: HEK293 cells
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150.0 KDa
纯化方法	Protein A purified
储存缓冲液	PBS, pH 7.2
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
分子类型	抗体

关联靶点（人）

TP53 Tchem 细胞肿瘤抗原 p53(Cellular tumor antigen p53) (0 活性数据)

点击查看靶蛋白信息： TP53

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: p53 Mouse mAb (Ab169235)

p53 Mouse mAb (Ab169235) - Western Blot
All lanes: p53 Mouse Antibody (Ab169235) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 52 kDa
Exposure time: 30.0 s

Flow Cytometry: p53 Mouse mAb (Ab169235)

p53 Mouse mAb (Ab169235) - Flow Cytometry 
Flow Cytometry analysis of HEK293 cells stained with p53 Mouse mAb (Ab169235) (Red) at 2.5 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue).

应用

应用名称	稀释比例
WB	1/1000 - 1/20000
Flow Cytometry	1/200 - 1/3

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0117098	分析证书	Ab169235
ZJ25F0117097	分析证书	Ab169235
ZJ25F0117096	分析证书	Ab169235

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