TNF alpha Mouse mAb

COA

级别和纯度:

≥95%(SDS-PAGE)
1.0 mg/mL

应用:

Capture Antibody
ELISA

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: C11/10B2
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab156547-10μg	10μg	现货
Ab156547-50μg	50μg	现货
Ab156547-100μg	100μg	现货
Ab156547-1mg	1mg	期货

查看相关系列

点击查看靶蛋白信息: TNF

Accession#:P01375 (17) Gene ID:7124 (17) Tnf (22) TNF alpha (8)

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基本信息

产品名称	TNF alpha Mouse mAb
别名	肿瘤坏死因子α \| 肿瘤坏死因子-α \| TNF alpha抗体 \| 小鼠抗人TNF \| TNF-α小鼠单克隆抗体 \| TNF-α抗体
英文别名	APC1 antibody \| APC1 protein antibody \| Cachectin antibody \| DIF antibody \| Differentiation inducing factor antibody \| Macrophage cytotoxic factor antibody \| Tnf antibody \| TNF superfamily member 2 antibody \| TNF superfamily, member 2 antibody \| TNF, macr
规格或纯度	ExactAb™, 已验证, 无动物源, 无载体, 无叠氮钠, 高性能, ≥95%(SDS-PAGE), 1.0 mg/mL
生化机理	Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
宿主种属	小鼠(Mouse)
特异性	Human TNF alpha
种属反应性	人(Human)
预测反应种属	Hylobates moloch,Nomascus leucogenys,Pan troglodytes,Piliocolobus tephrosceles,Symphalangus syndactylus
免疫原	Recombinant protein corresponding to Human TNF alpha
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
配对抗体	[Ab215301] TNF alpha Mouse mAb, Detection Antibody
储存缓冲液	PBS, pH 7.4
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
分子类型	抗体

关联靶点（人）

TNF Tclin 肿瘤坏死因子(Tumor necrosis factor) (0 活性数据)

点击查看靶蛋白信息： TNF

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Sandwich ELISA - TNF alpha Antibody Pair (Ab156547 & Ab215301)

Sandwich ELISA - TNF alpha standard curve
Sandwich ELISA detection of Recombinant Human TNF-α Protein (rp156007) using TNF alpha Mouse mAb (Ab156547) as capture antibody at concentration of 1.0 µg/mL. Biotinylated TNF alpha Mouse mAb (Ab215301) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 0.97 ng/mL - 1000.0 ng/mL

Indirect ELISA - TNF alpha Mouse mAb (Ab156547)

Indirect ELISA - TNF alpha standard curve
Immobilized Recombinant Human TNF-α Protein (rp156007) at 1.0 µg/mL overnight at 4℃. Coated protein probed with TNF alpha Mouse mAb (Ab156547). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC50: 59.41 ng/mL

应用