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Wnt1 Antibody

    应用:
  • IF/ICC
  • IHC
  • WB
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human), 猪(Pig)
  • 亚型: Rabbit IgG
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab134725-10μl
10μl 现货 Stock Image
Ab134725-50μl
50μL 现货 Stock Image
Ab134725-100μl
100μL 现货 Stock Image
Ab134725-1ml
1ml 期货 Stock Image

基本信息

产品名称 Wnt1 Antibody
别名 Anti- Wnt1抗体 | Wnt1多抗 | 信号通路Wnt1抗体 | Wnt1抗体
英文别名 Int-1 | INT1Proto-oncogene Int-1 homolog | proto-oncogene Wnt-1 | wingless-type MMTV integration site family, member 1 (oncogene INT1) | wingless-type MMTV integration site family, member 1 | Wnt1 | Wnt-1 | BMND16 antibody | INT1 antibody | OI15 antibody
规格或纯度 无载体, ExactAb™, 已验证, 高性能, 见COA
宿主种属 兔(Rabbit)
特异性 WNT1
种属反应性 人(Human),猪(Pig)
免疫原 Recombinant WNT1 expressed in E.coli (AA 28-370)
阳性对照 WB: HeLa and Jurkat cell lysates. IHC: Human kidney tissue. IF/ICC: MCF7 cells.
偶联 Unconjugated

产品属性

克隆类型 多克隆抗体
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4
防腐剂 0.02% Sodium azide
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

WNT1 Tbio 原癌基因Wnt-1(Proto-oncogene Wnt-1) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

Wnt1 Antibody (Ab134725) - Western Blot
All lanes: Wnt1 Antibody (Ab134725) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 40 kDa
Observed band size: 37 kDa
Exposure time: 42.0 s

Wnt1 Antibody (Ab134725) - IF/ICC
FITC staining on IF
Sample: Human MCF7 cells
Primary Ab: 20 μg/mL Wnt1 Antibody (Ab134725)
Second Ab: 1.5 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Wnt1 Antibody (Ab134725) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL Wnt1 Antibody (Ab134725)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

应用

应用名称 稀释比例
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

IF/ICC

5-20µg/mL

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C of A & Other Certificates(BSE/TSE, COO):
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找到5个结果

批号(Lot Number) 证书类型 货号
ZJ23F1202146 分析证书 Ab134725
ZJ23F1202147 分析证书 Ab134725
ZJ23F1202148 分析证书 Ab134725
ZJ24F1012131 分析证书 Ab134725
ZJ24F1011962 分析证书 Ab134725

引用文献

1. van Ooyen, A A, Kwee, V V and Nusse, R R..  (1985)  The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences..  The EMBO journal,  [PMID:2998762]
2. Fahiminiya, Somayyeh S and 5 more authors..  (2013)  Mutations in WNT1 are a cause of osteogenesis imperfecta..  Journal of medical genetics,  [PMID:23434763]
3. Keupp, Katharina K and 35 more authors..  (2013)  Mutations in WNT1 cause different forms of bone fragility..  American journal of human genetics,  (4): [PMID:23499309]
4. Pyott, Shawna M SM and 14 more authors..  (2013)  WNT1 mutations in families affected by moderately severe and progressive recessive osteogenesis imperfecta..  American journal of human genetics,  (4): [PMID:23499310]
5. Laine, Christine M CM and 22 more authors..  (2013)  WNT1 mutations in early-onset osteoporosis and osteogenesis imperfecta..  The New England journal of medicine,  (9): [PMID:23656646]
6. Mihara, Emiko E and 7 more authors..  (2016)  Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin..  eLife,  (23): [PMID:26902720]
7. Won, Joon Yeon JY and 7 more authors..  (2017)  Novel missense loss-of-function mutations of WNT1 in an autosomal recessive Osteogenesis imperfecta patient..  European journal of medical genetics,  [PMID:28528193]

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