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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab134725-10μl |
10μl |
现货 ![]() |
| |
| Ab134725-50μl |
50μL |
现货 ![]() |
| |
| Ab134725-100μl |
100μL |
现货 ![]() |
| |
| Ab134725-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | Wnt1 Antibody |
|---|---|
| 别名 | Anti- Wnt1抗体 | Wnt1多抗 | 信号通路Wnt1抗体 | Wnt1抗体 |
| 英文别名 | Int-1 | INT1Proto-oncogene Int-1 homolog | proto-oncogene Wnt-1 | wingless-type MMTV integration site family, member 1 (oncogene INT1) | wingless-type MMTV integration site family, member 1 | Wnt1 | Wnt-1 | BMND16 antibody | INT1 antibody | OI15 antibody |
| 规格或纯度 | 无载体, ExactAb™, 已验证, 高性能, 见COA |
| 宿主种属 | 兔(Rabbit) |
| 特异性 | WNT1 |
| 种属反应性 | 人(Human),猪(Pig) |
| 免疫原 | Recombinant WNT1 expressed in E.coli (AA 28-370) |
| 阳性对照 | WB: HeLa and Jurkat cell lysates. IHC: Human kidney tissue. IF/ICC: MCF7 cells. |
| 偶联 | Unconjugated |
| 克隆类型 | 多克隆抗体 |
|---|---|
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4 |
| 防腐剂 | 0.02% Sodium azide |
| 浓度 | 见COA |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
Wnt1 Antibody (Ab134725) - Western Blot
All lanes: Wnt1 Antibody (Ab134725) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 37 kDa
Exposure time: 42.0 s
Wnt1 Antibody (Ab134725) - IF/ICC
FITC staining on IF
Sample: Human MCF7 cells
Primary Ab: 20 μg/mL Wnt1 Antibody (Ab134725)
Second Ab: 1.5 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Wnt1 Antibody (Ab134725) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL Wnt1 Antibody (Ab134725)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| 应用名称 | 稀释比例 |
|---|---|
| IHC | 5-20 µg/mL |
| WB |
0.01-2 µg/mL
|
| IF/ICC | 5-20µg/mL |
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab134725 | |
| 分析证书 | Ab134725 | |
| 分析证书 | Ab134725 | |
| 分析证书 | Ab134725 | |
| 分析证书 | Ab134725 |
| 1. van Ooyen, A A, Kwee, V V and Nusse, R R.. (1985) The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences.. The EMBO journal, [PMID:2998762] |
| 2. Fahiminiya, Somayyeh S and 5 more authors.. (2013) Mutations in WNT1 are a cause of osteogenesis imperfecta.. Journal of medical genetics, [PMID:23434763] |
| 3. Keupp, Katharina K and 35 more authors.. (2013) Mutations in WNT1 cause different forms of bone fragility.. American journal of human genetics, (4): [PMID:23499309] |
| 4. Pyott, Shawna M SM and 14 more authors.. (2013) WNT1 mutations in families affected by moderately severe and progressive recessive osteogenesis imperfecta.. American journal of human genetics, (4): [PMID:23499310] |
| 5. Laine, Christine M CM and 22 more authors.. (2013) WNT1 mutations in early-onset osteoporosis and osteogenesis imperfecta.. The New England journal of medicine, (9): [PMID:23656646] |
| 6. Mihara, Emiko E and 7 more authors.. (2016) Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin.. eLife, (23): [PMID:26902720] |
| 7. Won, Joon Yeon JY and 7 more authors.. (2017) Novel missense loss-of-function mutations of WNT1 in an autosomal recessive Osteogenesis imperfecta patient.. European journal of medical genetics, [PMID:28528193] |