TBP Mouse mAb

COA

级别和纯度:

见COA

应用:

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse), 山羊(Goat), 猪(Pig), 绵羊(Sheep), 豚鼠(Guinea pig)
亚型: Mouse IgG2a
克隆号: C3
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab130268-10μl	10μl	现货
Ab130268-50μl	50μL	现货
Ab130268-100μl	100μL	现货
Ab130268-1ml	1ml	期货

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点击查看靶蛋白信息: TBP

Accession#:P20226 (4) Gene ID:6908 (4) TBP (4)

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基本信息

产品名称	TBP Mouse mAb
别名	TATA结合蛋白(内参)单克隆抗体 \| TATA结合蛋白单克隆抗体 \| TATA结合蛋白抗体 \| TBP 小鼠单克隆抗体 \| TBP 小鼠单抗
英文别名	GTF2D antibody \| GTF2D1 antibody \| HDL4 antibody \| MGC117320 antibody \| MGC126054 antibody \| MGC126055 antibody \| SCA17 antibody \| TATA binding factor antibody \| TATA box factor antibody \| TATA sequence binding protein antibody \| TATA sequence-binding pro
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属	小鼠(Mouse)
特异性	TBP
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse),山羊(Goat),猪(Pig),绵羊(Sheep),豚鼠(Guinea pig)
免疫原	Recombinant TBP expressed in E.coli (AA 25-339).
阳性对照	WB: Jurkat, HEK293, HeLa, K562, C6, NIH/3T3 cell lysates.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	C3
Format	Whole IgG
亚型	Mouse IgG2a
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A+G Affinity Chromatography
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4.
防腐剂	0.05% Proclin 300
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（12个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

TBP Tbio TATA盒结合蛋白(TATA-box-binding protein) (0 活性数据)

点击查看靶蛋白信息： TBP

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: TBP Mouse mAb (Ab130268)

TBP Mouse mAb (Ab130268) - Western Blot
All lanes: TBP Mouse mAb (Ab130268) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 38 kDa
Observed band size: 43&36 kDa
Exposure time: 5.1 s

应用

应用名称	稀释比例
WB	0.5-2 µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F0800899	分析证书	Ab130268
ZJ23F0800900	分析证书	Ab130268
ZJ24F0911361	分析证书	Ab130268

技术文档和文章

蛋白质印迹的上样对照

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一抗蛋白质印迹内参抗体

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Loading Controls for Western Blotting

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蛋白质印迹的一抗选择指南

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重组抗体单克隆抗体多克隆抗体蛋白质印迹内参抗体一抗选择

Guidelines of Primary Antibodies for Western Blotting

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Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

Guidelines of Primary Antibodies for Western Blotting

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Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

高分子量蛋白质印迹法

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免疫印迹生物缓冲液蛋白质印迹高分子量蛋白大分子蛋白蛋白质电泳 WB抗体

Protocol of Western Blotting For High Molecular Weights

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

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引用文献

1. Lee SK, Anzick SL, Choi JE, Bubendorf L, Guan XY, Jung YK, Kallioniemi OP, Kononen J, Trent JM, Azorsa D, Jhun BH, Cheong JH, Lee YC, Meltzer PS, Lee JW. (1999) A nuclear factor, ASC-2, as a cancer-amplified transcriptional coactivator essential for ligand-dependent transactivation by nuclear receptors in vivo.. J Biol Chem, 274 (48): (34283-93). [PMID:10567404]
2. Kao, C C CC and 5 more authors.. (1990) Cloning of a transcriptionally active human TATA binding factor.. Science (New York, N.Y.), (29): [PMID:2194289]
3. Peterson, M G MG, Tanese, N N, Pugh, B F BF and Tjian, R R.. (1990) Functional domains and upstream activation properties of cloned human TATA binding protein.. Science (New York, N.Y.), (29): [PMID:2363050]
4. Hoffman, A A and 6 more authors.. (1990) Highly conserved core domain and unique N terminus with presumptive regulatory motifs in a human TATA factor (TFIID).. Nature, (26): [PMID:2374612]
5. Veschambre, P P, Simard, P P and Jalinot, P P.. (1995) Evidence for functional interaction between the HIV-1 Tat transactivator and the TATA box binding protein in vivo.. Journal of molecular biology, (7): [PMID:7608968]
6. Comai, L L and 5 more authors.. (1994) Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits.. Science (New York, N.Y.), (23): [PMID:7801123]
7. Kashanchi, F F and 7 more authors.. (1994) Direct interaction of human TFIID with the HIV-1 transactivator tat.. Nature, (20): [PMID:8121496]
8. Ha, I I and 6 more authors.. (1993) Multiple functional domains of human transcription factor IIB: distinct interactions with two general transcription factors and RNA polymerase II.. Genes & development, [PMID:8504927]
9. Nikolov, D B DB and 5 more authors.. (1996) Crystal structure of a human TATA box-binding protein/TATA element complex.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:8643494]
10. Juo, Z S ZS and 5 more authors.. (1996) How proteins recognize the TATA box.. Journal of molecular biology, (16): [PMID:8757291]
11. Fukushima, A A and 5 more authors.. (1998) Characterization of functional domains of an embryonic stem cell coactivator UTF1 which are conserved and essential for potentiation of ATF-2 activity.. The Journal of biological chemistry, (2): [PMID:9748258]
12. LeRoy, G G, Orphanides, G G, Lane, W S WS and Reinberg, D D.. (1998) Requirement of RSF and FACT for transcription of chromatin templates in vitro.. Science (New York, N.Y.), (4): [PMID:9836642]
13. Rao, S S, Matsumura, A A, Yoon, J J and Simon, M C MC.. (1999) SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1.. The Journal of biological chemistry, (16): [PMID:10196196]
14. Eberhard, D D and Busslinger, M M.. (1999) The partial homeodomain of the transcription factor Pax-5 (BSAP) is an interaction motif for the retinoblastoma and TATA-binding proteins.. Cancer research, (1): [PMID:10197586]
15. Chang, C H CH, Scott, G K GK, Baldwin, M A MA and Benz, C C CC.. (1999) Exon 4-encoded acidic domain in the epithelium-restricted Ets factor, ESX, confers potent transactivating capacity and binds to TATA-binding protein (TBP).. Oncogene, (24): [PMID:10391676]
16. Koide, R R and 8 more authors.. (1999) A neurological disease caused by an expanded CAG trinucleotide repeat in the TATA-binding protein gene: a new polyglutamine disease?. Human molecular genetics, [PMID:10484774]
17. Tsai, F T FT and Sigler, P B PB.. (2000) Structural basis of preinitiation complex assembly on human pol II promoters.. The EMBO journal, (4): [PMID:10619841]
18. Yuan, C X CX and Gurley, W B WB.. (2000) Potential targets for HSF1 within the preinitiation complex.. Cell stress & chaperones, [PMID:11005381]
19. Zühlke, C C and 8 more authors.. (2001) Different types of repeat expansion in the TATA-binding protein gene are associated with a new form of inherited ataxia.. European journal of human genetics : EJHG, [PMID:11313753]
20. Gangloff, Y G YG and 9 more authors.. (2001) The TFIID components human TAF(II)140 and Drosophila BIP2 (TAF(II)155) are novel metazoan homologues of yeast TAF(II)47 containing a histone fold and a PHD finger.. Molecular and cellular biology, [PMID:11438666]
21. Nakamura, K K and 8 more authors.. (2001) SCA17, a novel autosomal dominant cerebellar ataxia caused by an expanded polyglutamine in TATA-binding protein.. Human molecular genetics, (1): [PMID:11448935]
22. Kamada, K K and 7 more authors.. (2001) Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex.. Cell, (13): [PMID:11461703]
23. Cabart, P P and Murphy, S S.. (2001) BRFU, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with TATA-binding protein.. The Journal of biological chemistry, (16): [PMID:11564744]
24. Silveira, I I and 24 more authors.. (2002) Trinucleotide repeats in 202 families with ataxia: a small expanded (CAG)n allele at the SCA17 locus.. Archives of neurology, [PMID:11939898]
25. Wang, P Jeremy PJ and Page, David C DC.. (2002) Functional substitution for TAF(II)250 by a retroposed homolog that is expressed in human spermatogenesis.. Human molecular genetics, (15): [PMID:12217962]
26. Hinkley, Craig S CS, Hirsch, Heather A HA, Gu, Liping L, LaMere, Brandon B and Henry, R William RW.. (2003) The small nuclear RNA-activating protein 190 Myb DNA binding domain stimulates TATA box-binding protein-TATA box recognition.. The Journal of biological chemistry, (16): [PMID:12621023]
27. Raha, Tamal T, Cheng, S W Grace SW and Green, Michael R MR.. (2005) HIV-1 Tat stimulates transcription complex assembly through recruitment of TBP in the absence of TAFs.. PLoS biology, [PMID:15719058]
28. Friedrich, J Karsten JK, Panov, Kostya I KI, Cabart, Pavel P, Russell, Jackie J and Zomerdijk, Joost C B M JC.. (2005) TBP-TAF complex SL1 directs RNA polymerase I pre-initiation complex formation and stabilizes upstream binding factor at the rDNA promoter.. The Journal of biological chemistry, (19): [PMID:15970593]
29. Di Pietro, Cinzia C and 22 more authors.. (2007) Genomics, evolution, and expression of TBPL2, a member of the TBP family.. DNA and cell biology, [PMID:17570761]
30. Liu, Lifeng L and 9 more authors.. (2009) MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity.. The Journal of biological chemistry, (20): [PMID:19106100]
31. Gouge, Jerome J and 8 more authors.. (2015) Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2.. Cell, (3): [PMID:26638071]
32. Louder, Robert K RK and 5 more authors.. (2016) Structure of promoter-bound TFIID and model of human pre-initiation complex assembly.. Nature, (31): [PMID:27007846]
33. He, Yuan Y and 6 more authors.. (2016) Near-atomic resolution visualization of human transcription promoter opening.. Nature, (19): [PMID:27193682]
34. Zhang, Mudan and 13 more authors.. (2018) Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4.. Frontiers in immunology, [PMID:30619292]

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所需的复溶浓度

TBP Mouse mAb

库存信息

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库存信息

基本信息

产品属性

关联靶点（人）

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

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引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器