STAT5a Antibody

COA

级别和纯度:

见COA

应用:

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 小鼠(Mouse), 猪(Pig)
亚型: Rabbit IgG
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab129464-10μl	10μl	现货
Ab129464-50μl	50μL	现货
Ab129464-100μl	100μL	现货
Ab129464-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: STAT5A

Accession#:P42229 (2) Gene ID:6776 (2) STAT5A (2)

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基本信息

产品名称	STAT5a Antibody
别名	信号转导和转录激活因子5a多克隆抗体 \| STAT5a 抗体
英文别名	Mammary gland factor antibody \| MGF antibody \| Signal transducer and activator of transcription 5A antibody \| STA5A_HUMAN antibody \| STAT 5 antibody \| STAT 5A antibody \| STAT5 antibody \| STAT5A antibody \| MGF \| signal transducer and activator of transcrip
规格或纯度	无载体, ExactAb™, 已验证, 高性能, 见COA
宿主种属	兔(Rabbit)
特异性	STAT5a
种属反应性	人(Human),小鼠(Mouse),猪(Pig)
免疫原	Recombinant STAT5a expressed in E.coli (AA 673-773)
阳性对照	WB: MOLT-4 and K562 cell lysates. IHC: Human lymph node tissue.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified and Protein A purified
物理外观	Liquid
储存缓冲液	10 mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

STAT5A Tchem 信号转导和转录激活因子 5A(Signal transducer and activator of transcription 5A) (0 活性数据)

点击查看靶蛋白信息： STAT5A

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

STAT5A Tchem Signal transducer and activator of transcription 5A (227 活性数据)

点击查看靶蛋白信息： STAT5A

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

STAT5A Tchem STAT5A/STAT5B (8 活性数据)

点击查看靶蛋白信息： STAT5A

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: STAT5a Antibody (Ab129464)

STAT5a Antibody (Ab129464) - Western Blot
All lanes: STAT5a Antibody (Ab129464) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 90 kDa
Observed band size: 90 kDa
Exposure time: 24.2 s

IHC: STAT5a Antibody (Ab129464)

STAT5a Antibody (Ab129464) - IHC
DAB staining on IHC
Sample: Human Lymph node Tissue
Primary Ab: 20 μg/mL STAT5a Antibody (Ab129464)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

应用

应用名称	稀释比例
IHC	5-20 µg/mL
WB	0.01-2 µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F1001317	分析证书	Ab129464
ZJ23F1001318	分析证书	Ab129464
ZJ24F1011794	分析证书	Ab129464

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

引用文献

1. Ioannidis S, Lamb ML, Wang T, Almeida L, Block MH, Davies AM, Peng B, Su M, Zhang HJ, Hoffmann E et al.. (2011) Discovery of 5-chloro-N2-[(1S)-1-(5-fluoropyrimidin-2-yl)ethyl]-N4-(5-methyl-1H-pyrazol-3-yl)pyrimidine-2,4-diamine (AZD1480) as a novel inhibitor of the Jak/Stat pathway.. J Med Chem, 54 (1): (262-76). [PMID:21138246]
2. Galkin AV, Melnick JS, Kim S, Hood TL, Li N, Li L, Xia G, Steensma R, Chopiuk G, Jiang J et al.. (2007) Identification of NVP-TAE684, a potent, selective, and efficacious inhibitor of NPM-ALK.. Proc Natl Acad Sci USA, 104 (1): (270-5). [PMID:17185414]
3. Changelian PS, Flanagan ME, Ball DJ, Kent CR, Magnuson KS, Martin WH, Rizzuti BJ, Sawyer PS, Perry BD, Brissette WH et al.. (2003) Prevention of organ allograft rejection by a specific Janus kinase 3 inhibitor.. Science, 302 (5646): (875-8). [PMID:14593182]
4. Jiang JK, Ghoreschi K, Deflorian F, Chen Z, Perreira M, Pesu M, Smith J, Nguyen DT, Liu EH, Leister W, Costanzi S, O'Shea JJ, Thomas CJ.. (2008) Examining the chirality, conformation and selective kinase inhibition of 3-((3R,4R)-4-methyl-3-(methyl(7H-pyrrolo[2,3-d]pyrimidin-4-yl)amino)piperidin-1-yl)-3-oxopropanenitrile (CP-690,550).. J Med Chem, 51 (24): (8012-8018). [PMID:19053756] [10.1021/jm801142b]
5. Wilson LJ, Malaviya R, Yang C, Argentieri R, Wang B, Chen X, Murray WV, Cavender D.. (2009) Synthetic staurosporines via a ring closing metathesis strategy as potent JAK3 inhibitors and modulators of allergic responses.. Bioorg Med Chem Lett, 19 (12): (3333-3338). [PMID:19427203] [10.1016/j.bmcl.2009.04.039]
6. Ren X, Duan L, He Q, Zhang Z, Zhou Y, Wu D, Pan J, Pei D, Ding K, Ding K.. (2010) Identification of Niclosamide as a New Small-Molecule Inhibitor of the STAT3 Signaling Pathway.. ACS Med Chem Lett, 1 (9): (454-459). [PMID:24900231] [10.1021/ml100146z]
7. Mandal PK, Gao F, Lu Z, Ren Z, Ramesh R, Birtwistle JS, Kaluarachchi KK, Chen X, Bast RC, Liao WS, McMurray JS.. (2011) Potent and selective phosphopeptide mimetic prodrugs targeted to the Src homology 2 (SH2) domain of signal transducer and activator of transcription 3.. J Med Chem, 54 (10): (3549-3563). [PMID:21486047] [10.1021/jm2000882]
8. Kumar A, Shah BA, Singh S, Hamid A, Singh SK, Sethi VK, Saxena AK, Singh J, Taneja SC.. (2012) Acyl derivatives of boswellic acids as inhibitors of NF-κB and STATs.. Bioorg Med Chem Lett, 22 (1): (431-435). [PMID:22123322] [10.1016/j.bmcl.2011.10.112]
9. Page BD, Khoury H, Laister RC, Fletcher S, Vellozo M, Manzoli A, Yue P, Turkson J, Minden MD, Gunning PT.. (2012) Small molecule STAT5-SH2 domain inhibitors exhibit potent antileukemia activity.. J Med Chem, 55 (3): (1047-1055). [PMID:22148584] [10.1021/jm200720n]
10. Page BD, Croucher DC, Li ZH, Haftchenary S, Jimenez-Zepeda VH, Atkinson J, Spagnuolo PA, Wong YL, Colaguori R, Lewis AM, Schimmer AD, Trudel S, Gunning PT.. (2013) Inhibiting aberrant signal transducer and activator of transcription protein activation with tetrapodal, small molecule Src homology 2 domain binders: promising agents against multiple myeloma.. J Med Chem, 56 (18): (7190-7200). [PMID:23968501] [10.1021/jm3017255]
11. Haftchenary S, Luchman HA, Jouk AO, Veloso AJ, Page BD, Cheng XR, Dawson SS, Grinshtein N, Shahani VM, Kerman K, Kaplan DR, Griffin C, Aman AM, Al-Awar R, Weiss S, Gunning PT.. (2013) Potent Targeting of the STAT3 Protein in Brain Cancer Stem Cells: A Promising Route for Treating Glioblastoma.. ACS Med Chem Lett, 4 (11): (1102-1107). [PMID:24900612] [10.1021/ml4003138]
12. Chen H, Guan Y, Yuan G, Zhang Q, Jing N.. (2014) A perylene derivative regulates HIF-1α and Stat3 signaling pathways.. Bioorg Med Chem, 22 (4): (1496-1505). [PMID:24485121] [10.1016/j.bmc.2013.10.018]
13. Romagnoli R, Baraldi PG, Prencipe F, Lopez-Cara C, Rondanin R, Simoni D, Hamel E, Grimaudo S, Pipitone RM, Meli M, Tolomeo M.. (2016) Novel iodoacetamido benzoheterocyclic derivatives with potent antileukemic activity are inhibitors of STAT5 phosphorylation.. Eur J Med Chem, 108 (39-52). [PMID:26629859] [10.1016/j.ejmech.2015.11.022]
14. Juen L, Brachet-Botineau M, Parmenon C, Bourgeais J, Hérault O, Gouilleux F, Viaud-Massuard MC, Prié G.. (2017) New Inhibitor Targeting Signal Transducer and Activator of Transcription 5 (STAT5) Signaling in Myeloid Leukemias.. J Med Chem, 60 (14): (6119-6136). [PMID:28654259] [10.1021/acs.jmedchem.7b00369]
15. Kim HS, Kim T, Ko H, Lee J, Kim YS, Suh YG.. (2017) Identification of galiellalactone-based novel STAT3-selective inhibitors with cytotoxic activities against triple-negative breast cancer cell lines.. Bioorg Med Chem, 25 (19): (5032-5040). [PMID:28705432] [10.1016/j.bmc.2017.06.036]
16. Berg A, Berg T.. (2017) A small-molecule screen identifies the antitrypanosomal agent suramin and analogues NF023 and NF449 as inhibitors of STAT5a/b.. Bioorg Med Chem Lett, 27 (15): (3349-3352). [PMID:28624143] [10.1016/j.bmcl.2017.06.012]
17. Guo J, Yu W, Cai G, Zhang W, Li S, Zhu J, Song D, Kong L.. (2018) Discovery of new benzensulfonamide derivatives as tripedal STAT3 inhibitors.. Eur J Med Chem, 151 (752-764). [PMID:29674294] [10.1016/j.ejmech.2018.03.053]
18. Zhou H, Bai L, Xu R, Zhao Y, Chen J, McEachern D, Chinnaswamy K, Wen B, Dai L, Kumar P, Yang CY, Liu Z, Wang M, Liu L, Meagher JL, Yi H, Sun D, Stuckey JA, Wang S.. (2019) Structure-Based Discovery of SD-36 as a Potent, Selective, and Efficacious PROTAC Degrader of STAT3 Protein.. J Med Chem, 62 (24): (11280-11300). [PMID:31747516] [10.1021/acs.jmedchem.9b01530]
19. Huang MR, Hsu YL, Lin TC, Cheng TJ, Li LW, Tseng YW, Chou YS, Liu JH, Pan SH, Fang JM, Wong CH.. (2019) Structure-guided development of purine amide, hydroxamate, and amidoxime for the inhibition of non-small cell lung cancer.. Eur J Med Chem, 181 (111551-111551). [PMID:31376567] [10.1016/j.ejmech.2019.07.054]
20. Kaoud TS,Mohassab AM,Hassan HA,Yan C,Van Ravenstein SX,Abdelhamid D,Dalby KN,Abdel-Aziz M. (2020) NO-releasing STAT3 inhibitors suppress BRAF-mutant melanoma growth.. Eur J Med Chem, 186 (111885-111885). [PMID:31784187] [10.1016/j.ejmech.2019.111885]
21. Schoepf AM,Salcher S,Obexer P,Gust R. (2020) Identification and development of non-cytotoxic cell death modulators: Impact of sartans and derivatives on PPARγ activation and on growth of imatinib-resistant chronic myelogenous leukemia cells.. Eur J Med Chem, 195 (112258-112258). [PMID:32272420] [10.1016/j.ejmech.2020.112258]
22. Schoepf AM, Salcher S, Obexer P, Gust R.. (2021) Tackling resistance in chronic myeloid leukemia: Novel cell death modulators with improved efficacy.. Eur J Med Chem, 216 (113285-113285). [PMID:33662676] [10.1016/j.ejmech.2021.113285]
23. Hou, J J, Schindler, U U, Henzel, W J WJ, Wong, S C SC and McKnight, S L SL.. (1995) Identification and purification of human Stat proteins activated in response to interleukin-2.. Immunity, [PMID:7719937]
24. Litterst, Claudia M CM, Kliem, Stefanie S, Marilley, Dominique D and Pfitzner, Edith E.. (2003) NCoA-1/SRC-1 is an essential coactivator of STAT5 that binds to the FDL motif in the alpha-helical region of the STAT5 transactivation domain.. The Journal of biological chemistry, (14): [PMID:12954634]
25. Taketani, Takeshi T and 9 more authors.. (2004) FLT3 mutations in the activation loop of tyrosine kinase domain are frequently found in infant ALL with MLL rearrangements and pediatric ALL with hyperdiploidy.. Blood, (1): [PMID:14504097]
26. Rönnstrand, L L.. (2004) Signal transduction via the stem cell factor receptor/c-Kit.. Cellular and molecular life sciences : CMLS, [PMID:15526160]
27. Williams, Christopher C CC and 6 more authors.. (2004) The ERBB4/HER4 receptor tyrosine kinase regulates gene expression by functioning as a STAT5A nuclear chaperone.. The Journal of cell biology, (8): [PMID:15534001]
28. Martens, Nele N and 5 more authors.. (2005) Suppressor of cytokine signaling 7 inhibits prolactin, growth hormone, and leptin signaling by interacting with STAT5 or STAT3 and attenuating their nuclear translocation.. The Journal of biological chemistry, (8): [PMID:15677474]
29. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196]
30. Chaix, Amandine A and 5 more authors.. (2011) Mechanisms of STAT protein activation by oncogenic KIT mutants in neoplastic mast cells.. The Journal of biological chemistry, (25): [PMID:21135090]

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STAT5a Antibody

库存信息

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库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器