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SOD2/MnSOD Antibody

SOD2/MnSOD Antibody

级别和纯度:

见COA

应用:

IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 小鼠(Mouse)
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货	价格	数量
Ab128615-10μl	10μl	现货
Ab128615-50μl	50μL	现货
Ab128615-100μl	100μL	现货
Ab128615-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: SOD2

Accession#:P04179 (5) Gene ID:6648 (5) MnSOD (2) SOD2 (5)

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main product photo

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基本信息

产品名称	SOD2/MnSOD Antibody
别名	抗SOD2/MnSOD抗体 \| SOD2/MnSOD抗体
英文别名	EC 1.15.1.1 \| indophenoloxidase B \| IPOB \| IPO-B \| manganese-containing superoxide dismutase \| mangano-superoxide dismutase \| Mn SOD \| Mn superoxide dismutase \| MnSOD \| MVCD6 \| SOD, Mitochodrial \| SOD2 \| superoxide dismutase [Mn], mitochondrial \| superoxi
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属	兔(Rabbit)
特异性	SOD2
种属反应性	人(Human),小鼠(Mouse)
免疫原	Recombinant SOD2 expressed in E.coli (AA 25-222).
阳性对照	WB: HeLa, MCF7, HEK293, A431, A549, Hep G2, K562 and NIH/3T3 cell lysates. IHC: Human prostate cancer, breast cancer, kidney, skin cancer, colorectal cancer, liver tissues.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified + Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂	0.05% Proclin 300
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

SOD2 Tbio 超氧化物歧化酶 [Mn]，线粒体(Superoxide dismutase [Mn], mitochondrial) (0 活性数据)

点击查看靶蛋白信息： SOD2

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - Western Blot
All lanes: SOD2/MnSOD Antibody (Ab128615) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 1.3 s

IHC: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Skin cancer Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: SOD2/MnSOD Antibody (Ab128615)

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

应用

应用名称	稀释比例
IHC	5-20 µg/mL
WB	0.5-3 µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F1101550	分析证书	Ab128615
ZJ23F1101549	分析证书	Ab128615
ZJ24F1011852	分析证书	Ab128615

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

可替换产品

引用文献

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
2. Borgstahl, G E GE and 5 more authors.. (1992) The structure of human mitochondrial manganese superoxide dismutase reveals a novel tetrameric interface of two 4-helix bundles.. Cell, (2): [PMID:1394426]
3. and Church, S L SL.. (1990) Manganese superoxide dismutase: nucleotide and deduced amino acid sequence of a cDNA encoding a new human transcript.. Biochimica et biophysica acta, (23): [PMID:1699607]
4. St Clair, D K DK and Holland, J C JC.. (1991) Complementary DNA encoding human colon cancer manganese superoxide dismutase and the expression of its gene in human cells.. Cancer research, (1): [PMID:1988135]
5. Wispé, J R JR and 5 more authors.. (1989) Synthesis and processing of the precursor for human mangano-superoxide dismutase.. Biochimica et biophysica acta, (19): [PMID:2462451]
6. Ho, Y S YS and Crapo, J D JD.. (1988) Isolation and characterization of complementary DNAs encoding human manganese-containing superoxide dismutase.. FEBS letters, (14): [PMID:2831093]
7. and Heckl, K K.. (1988) Isolation of cDNAs encoding human manganese superoxide dismutase.. Nucleic acids research, (11): [PMID:3399391]
8. Beck, Y Y and 5 more authors.. (1987) Human Mn superoxide dismutase cDNA sequence.. Nucleic acids research, (11): [PMID:3684581]
9. Barra, D D and 6 more authors.. (1984) The primary structure of human liver manganese superoxide dismutase.. The Journal of biological chemistry, (25): [PMID:6386798]
10. Kovalyov, L I LI and 6 more authors.. (1995) The major protein expression profile and two-dimensional protein database of human heart.. Electrophoresis, [PMID:7498159]
11. Wan, X S XS, Devalaraja, M N MN and St Clair, D K DK.. (1994) Molecular structure and organization of the human manganese superoxide dismutase gene.. DNA and cell biology, [PMID:7702755]
12. Corbett, J M JM, Wheeler, C H CH, Baker, C S CS, Yacoub, M H MH and Dunn, M J MJ.. (1994) The human myocardial two-dimensional gel protein database: update 1994.. Electrophoresis, [PMID:7895732]
13. Borgstahl, G E GE and 8 more authors.. (1996) Human mitochondrial manganese superoxide dismutase polymorphic variant Ile58Thr reduces activity by destabilizing the tetrameric interface.. Biochemistry, (9): [PMID:8605177]
14. Rasmussen, R K RK and 6 more authors.. Two-dimensional electrophoretic analysis of human breast carcinoma proteins: mapping of proteins that bind to the SH3 domain of mixed lineage kinase MLK2.. Electrophoresis, [PMID:9150946]
15. Guan, Y Y and 9 more authors.. (1998) Crystal structure of Y34F mutant human mitochondrial manganese superoxide dismutase and the functional role of tyrosine 34.. Biochemistry, (7): [PMID:9537987]
16. Hsieh, Y Y and 9 more authors.. (1998) Probing the active site of human manganese superoxide dismutase: the role of glutamine 143.. Biochemistry, (7): [PMID:9537988]
17. Zhao, J J, Lahiri-Chatterjee, M M, Sharma, Y Y and Agarwal, R R.. (2000) Inhibitory effect of a flavonoid antioxidant silymarin on benzoyl peroxide-induced tumor promotion, oxidative stress and inflammatory responses in SENCAR mouse skin.. Carcinogenesis, [PMID:10753220]
18. Lévêque, V J VJ and 6 more authors.. (2000) Multiple replacements of glutamine 143 in human manganese superoxide dismutase: effects on structure, stability, and catalysis.. Biochemistry, (20): [PMID:10852710]
19. Hearn, A S AS and 6 more authors.. (2001) Kinetic analysis of product inhibition in human manganese superoxide dismutase.. Biochemistry, (9): [PMID:11580280]
20. Nomiyama, Takashi T and 8 more authors.. (2003) The polymorphism of manganese superoxide dismutase is associated with diabetic nephropathy in Japanese type 2 diabetic patients.. Journal of human genetics, [PMID:12624725]
21. Xu, Shanqin S and 11 more authors.. (2006) Detection of sequence-specific tyrosine nitration of manganese SOD and SERCA in cardiovascular disease and aging.. American journal of physiology. Heart and circulatory physiology, [PMID:16399855]
22. Liu, Limei L and 10 more authors.. (2009) The manganese superoxide dismutase Val16Ala polymorphism is associated with decreased risk of diabetic nephropathy in Chinese patients with type 2 diabetes.. Molecular and cellular biochemistry, [PMID:18989629]
23. Perry, J Jefferson P JJ and 5 more authors.. (2009) Contribution of human manganese superoxide dismutase tyrosine 34 to structure and catalysis.. Biochemistry, (21): [PMID:19265433]
24. Goitre, Luca L and 6 more authors.. (2010) KRIT1 regulates the homeostasis of intracellular reactive oxygen species.. PloS one, (26): [PMID:20668652]
25. Kim, Myung-Sun MS, Ramakrishna, Suresh S, Lim, Key-Hwan KH, Kim, Jun-Hyun JH and Baek, Kwang-Hyun KH.. (2011) Protein stability of mitochondrial superoxide dismutase SOD2 is regulated by USP36.. Journal of cellular biochemistry, [PMID:21268071]

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