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Ab127693-10μl

10μl

期货

Ab127693-50μl

50μL

期货

Ab127693-100μl

100μL

期货

Ab127693-1ml

1ml

期货

基本信息

产品名称	SIRT5 Mouse mAb
别名	沉默调节蛋白5(SIRT5)单克隆抗体
英文别名	EC 2.3.1.- \| Regulatory protein SIR2 homolog 5 \| SIR2-like protein 5 \| NAD dependent deacetylase sirtuin 5antibody \| NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody \| NAD dependent protein deacylase sirtuin 5 mitochond
规格或纯度	见COA
生化机理	NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
宿主种属	小鼠(Mouse)
特异性	SIRT5
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	Recombinant full length protein of human SIRT5 produced in HEK293.
阳性对照	WB: HEK293T transfected with pCMV6-ENTRY SIRT5 cDNA for 48 hrs, HepG2, HeLa, A549, COS7, PC12, MCF7 lysates. IHC: Human Kidney tissue. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY SIRT5. Flow Cytometry: HEK293T cells transfected with pCMV6-
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 1% BSA, 0.02% Sodium azide, pH 7.3
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。
分子类型	抗体

关联靶点（人）

SIRT5 Tchem NAD 依赖性蛋白去酰化酶 sirtuin-5，线粒体(NAD-dependent protein deacylase sirtuin-5, mitochondrial) (0 活性数据)

点击查看靶蛋白信息： SIRT5

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

SIRT5 Tchem NAD-dependent protein deacylase sirtuin-5, mitochondrial (1056 活性数据)

点击查看靶蛋白信息： SIRT5

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

应用

应用名称	稀释比例
WB	1/500-1/2000
IF/ICC	1:100
Flow Cytometry	1/100
IHC	1/150

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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引用文献

1. Maurer B, Rumpf T, Scharfe M, Stolfa DA, Schmitt ML, He W, Verdin E, Sippl W, Jung M. (2012) Inhibitors of the NAD(+)-Dependent Protein Desuccinylase and Demalonylase Sirt5.. ACS Med Chem Lett, 3 (12): (1050-3). [PMID:24900427]
2. Ai T, Wilson DJ, More SS, Xie J, Chen L. (2016) 5-((3-Amidobenzyl)oxy)nicotinamides as Sirtuin 2 Inhibitors.. J Med Chem, 59 (7): (2928-41). [PMID:26982234]
3. Benjamin Suenkel,Frank Fischer,Clemens Steegborn. (2012-12-01) Inhibition of the human deacylase Sirtuin 5 by the indole GW5074.. Bioorganic & medicinal chemistry letters, 23 ((1)): (143-146). [PMID:23195732]
4. Jeongsoon Park,Yue Chen,Daniel X Tishkoff,Chao Peng,Minjia Tan,Lunzhai Dai,Zhongyu Xie,Yi Zhang,Bernadette M M Zwaans,Mary E Skinner,David B Lombard,Yingming Zhao. (2013-06-29) SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways.. Molecular cell, 50 ((6)): (919-930). [PMID:23806337]
5. Xin Yang,Zhe Wang,Xin Li,Boya Liu,Minghui Liu,Lu Liu,Shuaiyi Chen,Mengmeng Ren,Yankun Wang,Miao Yu,Bo Wang,Junhua Zou,Wei-Guo Zhu,Yuxin Yin,Wei Gu,Jianyuan Luo. (2017-11-29) SHMT2 Desuccinylation by SIRT5 Drives Cancer Cell Proliferation.. Cancer research, 78 ((2)): (372-386). [PMID:29180469]
6. Zang W, Hao Y, Wang Z, Zheng W.. (2015) Novel thiourea-based sirtuin inhibitory warheads.. Bioorg Med Chem Lett, 25 (16): (3319-3324). [PMID:26081291] [10.1016/j.bmcl.2015.05.058]
7. Chen D, Zheng W.. (2016) Cyclic peptide-based potent and selective SIRT1/2 dual inhibitors harboring Nε-thioacetyl-lysine.. Bioorg Med Chem Lett, 26 (21): (5234-5239). [PMID:27707605] [10.1016/j.bmcl.2016.09.055]
8. Yang LL, Wang HL, Zhong L, Yuan C, Liu SY, Yu ZJ, Liu S, Yan YH, Wu C, Wang Y, Wang Z, Yu Y, Chen Q, Li GB.. (2018) X-ray crystal structure guided discovery of new selective, substrate-mimicking sirtuin 2 inhibitors that exhibit activities against non-small cell lung cancer cells.. Eur J Med Chem, 155 (806-823). [PMID:29957526] [10.1016/j.ejmech.2018.06.041]
9. Li Y, Zhou Y, Wang F, Chen X, Wang C, Wang J, Liu T, Li Y, He B.. (2018) SIRT4 is the last puzzle of mitochondrial sirtuins.. Bioorg Med Chem, 26 (14): (3861-3865). [PMID:30033389] [10.1016/j.bmc.2018.07.031]
10. Gromek SM, deMayo JA, Maxwell AT, West AM, Pavlik CM, Zhao Z, Li J, Wiemer AJ, Zweifach A, Balunas MJ.. (2016) Synthesis and biological evaluation of santacruzamate A analogues for anti-proliferative and immunomodulatory activity.. Bioorg Med Chem, 24 (21): (5183-5196). [PMID:27614919] [10.1016/j.bmc.2016.08.040]
11. Chen X, Sun W, Huang S, Zhang H, Lin G, Li H, Qiao J, Li L, Yang S.. (2020) Discovery of Potent Small-Molecule SIRT6 Activators: Structure-Activity Relationship and Anti-Pancreatic Ductal Adenocarcinoma Activity.. J Med Chem, 63 (18): (10474-10495). [PMID:32787077] [10.1021/acs.jmedchem.0c01183]
12. Yang LL,Wang HL,Yan YH,Liu S,Yu ZJ,Huang MY,Luo Y,Zheng X,Yu Y,Li GB. (2020) Sensitive fluorogenic substrates for sirtuin deacylase inhibitor discovery.. Eur J Med Chem, 192 (112201-112201). [PMID:32163813] [10.1016/j.ejmech.2020.112201]
13. and Frye, R A RA.. (1999) Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity.. Biochemical and biophysical research communications, (24): [PMID:10381378]
14. Michishita, Eriko E, Park, Jean Y JY, Burneskis, Jenna M JM, Barrett, J Carl JC and Horikawa, Izumi I.. (2005) Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins.. Molecular biology of the cell, [PMID:16079181]
15. Heltweg, Birgit B and 10 more authors.. (2006) Antitumor activity of a small-molecule inhibitor of human silent information regulator 2 enzymes.. Cancer research, (15): [PMID:16618762]
16. Schlicker, Christine C and 5 more authors.. (2008) Substrates and regulation mechanisms for the human mitochondrial sirtuins Sirt3 and Sirt5.. Journal of molecular biology, (10): [PMID:18680753]
17. Matsushita, Nobuko N and 7 more authors.. (2011) Distinct regulation of mitochondrial localization and stability of two human Sirt5 isoforms.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:21143562]
18. Peng, Chao C and 18 more authors.. (2011) The first identification of lysine malonylation substrates and its regulatory enzyme.. Molecular & cellular proteomics : MCP, [PMID:21908771]
19. Du, Jintang J and 16 more authors.. (2011) Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase.. Science (New York, N.Y.), (11): [PMID:22076378]
20. Zhou, Yeyun Y and 6 more authors.. (2012) The bicyclic intermediate structure provides insights into the desuccinylation mechanism of human sirtuin 5 (SIRT5).. The Journal of biological chemistry, (17): [PMID:22767592]
21. Szczepankiewicz, Bruce G BG and 6 more authors.. (2012) Synthesis of carba-NAD and the structures of its ternary complexes with SIRT3 and SIRT5.. The Journal of organic chemistry, (7): [PMID:22849721]
22. Fischer, Frank F and 5 more authors.. (2012) Sirt5 deacylation activities show differential sensitivities to nicotinamide inhibition.. PloS one, [PMID:23028781]
23. Gertz, Melanie M and 10 more authors.. (2012) A molecular mechanism for direct sirtuin activation by resveratrol.. PloS one, [PMID:23185430]
24. Wirth, Martina and 9 more authors.. (2013) Mitochondrial SIRT4-type proteins in Caenorhabditis elegans and mammals interact with pyruvate carboxylase and other acetylated biotin-dependent carboxylases.. Mitochondrion, [PMID:23438705]
25. Lin, Zhi-Feng ZF and 8 more authors.. (2013) SIRT5 desuccinylates and activates SOD1 to eliminate ROS.. Biochemical and biophysical research communications, (8): [PMID:24140062]
26. Tan, Minjia M and 27 more authors.. (2014) Lysine glutarylation is a protein posttranslational modification regulated by SIRT5.. Cell metabolism, (1): [PMID:24703693]

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SIRT5 Mouse mAb

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质检证书（CoA,COO,BSE/TSE 和分析图谱)

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