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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab122503-10μl |
10μl |
现货 ![]() |
| |
| Ab122503-50μl |
50μL |
现货 ![]() |
| |
| Ab122503-100μl |
100μL |
期货 ![]() |
| |
| Ab122503-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | Recombinant Podoplanin Antibody |
|---|---|
| 别名 | Podoplanin抗体 | 平足蛋白抗体 | 淋巴管内皮细胞蛋白抗体 | 重组足足蛋白抗体 |
| 英文别名 | Aggrus antibody | Glycoprotein36 KD antibody | Glycoprotein 36 antibody | gp 36 antibody | GP 38 antibody | GP 40 antibody | gp36 antibody | GP38 antibody | GP40 antibody | HT1A 1 antibody | HT1A1 antibody | hT1alpha 1 antibody | hT1alpha 2 antibody | hT1 |
| 规格或纯度 | ExactAb™, Validated, 重组, 0.93 mg/mL |
| 宿主种属 | 兔(Rabbit) |
| 特异性 | Podoplanin |
| 种属反应性 | 人(Human),大鼠(Rat),小鼠(Mouse) |
| 免疫原 | A synthetic peptide derived from human Podoplanin (AA 121-162) |
| 阳性对照 | WB: Mouse lung, U-2 OS and NIH/3T3 lysates. |
| 偶联 | Unconjugated |
| 克隆类型 | 重组抗体 |
|---|---|
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4 |
| 防腐剂 | 0.02% Sodium azide |
| 浓度 | 0.93 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
Recombinant Podoplanin Antibody (Ab122503) - Western Blot
All lanes: Recombinant Podoplanin Antibody (Ab122503) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 38 kDa
Exposure time: 60.0 s
Recombinant Podoplanin Antibody (Ab122503) - Western Blot
All lanes: Recombinant Podoplanin Antibody (Ab122503) at 1/1000 dilution
Samples: Lysates at 15 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 37 kDa
Exposure time: 60.0 s
| 应用名称 | 稀释比例 |
|---|---|
| WB | 1/500-1/1000 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab122503 | |
| 分析证书 | Ab122503 |
| 1. Ma, T T, Yang, B B, Matthay, M A MA and Verkman, A S AS.. (1998) Evidence against a role of mouse, rat, and two cloned human t1alpha isoforms as a water channel or a regulator of aquaporin-type water channels.. American journal of respiratory cell and molecular biology, [PMID:9651190] |
| 2. Zimmer, G G and 6 more authors.. (1999) Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium.. The Biochemical journal, (15): [PMID:10393083] |
| 3. Kato, Yukinari Y and 6 more authors.. (2003) Molecular identification of Aggrus/T1alpha as a platelet aggregation-inducing factor expressed in colorectal tumors.. The Journal of biological chemistry, (19): [PMID:14522983] |
| 4. Kerjaschki, Dontscho D and 13 more authors.. (2004) Lymphatic neoangiogenesis in human kidney transplants is associated with immunologically active lymphocytic infiltrates.. Journal of the American Society of Nephrology : JASN, [PMID:14978162] |
| 5. Kaneko, Mika M and 5 more authors.. (2004) Functional sialylated O-glycan to platelet aggregation on Aggrus (T1alpha/Podoplanin) molecules expressed in Chinese hamster ovary cells.. The Journal of biological chemistry, (10): [PMID:15231832] |
| 6. Martín-Villar, Ester E and 6 more authors.. (2005) Characterization of human PA2.26 antigen (T1alpha-2, podoplanin), a small membrane mucin induced in oral squamous cell carcinomas.. International journal of cancer, (1): [PMID:15515019] |
| 7. Otsuki, Tetsuji T and 24 more authors.. (2005) Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.. DNA research : an international journal for rapid publication of reports on genes and genomes, [PMID:16303743] |
| 8. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 9. Martín-Villar, Ester E and 5 more authors.. (2006) Podoplanin binds ERM proteins to activate RhoA and promote epithelial-mesenchymal transition.. Journal of cell science, (1): [PMID:17046996] |
| 10. Kaneko, Mika Kato MK and 12 more authors.. (2007) Functional glycosylation of human podoplanin: glycan structure of platelet aggregation-inducing factor.. FEBS letters, (23): [PMID:17222411] |
| 11. Suzuki-Inoue, Katsue K and 8 more authors.. (2007) Involvement of the snake toxin receptor CLEC-2, in podoplanin-mediated platelet activation, by cancer cells.. The Journal of biological chemistry, (7): [PMID:17616532] |
| 12. Christou, Charita M CM and 9 more authors.. (2008) Renal cells activate the platelet receptor CLEC-2 through podoplanin.. The Biochemical journal, (1): [PMID:18215137] |
| 13. Nakazawa, Youya Y and 7 more authors.. (2008) Tetraspanin family member CD9 inhibits Aggrus/podoplanin-induced platelet aggregation and suppresses pulmonary metastasis.. Blood, (1): [PMID:18541721] |
| 14. Cueni, Leah N LN and Detmar, Michael M.. (2009) Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions.. Experimental cell research, (10): [PMID:19268462] |
| 15. Martín-Villar, Ester E and 7 more authors.. (2010) Podoplanin associates with CD44 to promote directional cell migration.. Molecular biology of the cell, [PMID:20962267] |
| 16. Fernández-Muñoz, Beatriz B and 6 more authors.. (2011) The transmembrane domain of podoplanin is required for its association with lipid rafts and the induction of epithelial-mesenchymal transition.. The international journal of biochemistry & cell biology, [PMID:21376833] |
| 17. Tsuneki, Masayuki M and 9 more authors.. (2013) Extracellular heat shock protein A9 is a novel interaction partner of podoplanin in oral squamous cell carcinoma cells.. Biochemical and biophysical research communications, (26): [PMID:23541579] |
| 18. Yurrita, Maria M MM and 5 more authors.. (2014) Podoplanin is a substrate of presenilin-1/γ-secretase.. The international journal of biochemistry & cell biology, [PMID:24275092] |
| 19. Nagae, Masamichi M and 5 more authors.. (2014) A platform of C-type lectin-like receptor CLEC-2 for binding O-glycosylated podoplanin and nonglycosylated rhodocytin.. Structure (London, England : 1993), (2): [PMID:25458834] |
| 20. Martín-Villar, E E and 6 more authors.. (2015) Podoplanin mediates ECM degradation by squamous carcinoma cells through control of invadopodia stability.. Oncogene, (20): [PMID:25486435] |