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抗体
多克隆抗体
PKM Antibody

PKM Antibody

级别和纯度:

见COA

应用:

IF/ICC
IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Rabbit IgG
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货	价格	数量
Ab122143-10μl	10μl	现货
Ab122143-50μl	50μL	现货
Ab122143-100μl	100μL	现货
Ab122143-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: PKM

Accession#:P14618 (2) Gene ID:5315 (2) PKM (3) PKM2 (2)

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基本信息

产品名称	PKM Antibody
别名	M2型丙酮酸激酶多克隆抗体 \| PKM 抗体
英文别名	CTHBP antibody \| Cytosolic thyroid hormone-binding protein antibody \| KPYM_HUMAN antibody \| OIP-3 antibody \| Opa-interacting protein 3 antibody \| p58 antibody \| pkm antibody \| PKM1 antibody \| PKM2 antibody \| Pyruvate kinase 2/3 antibody \| Pyruvate kinase
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属	兔(Rabbit)
特异性	PKM
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	Recombinant PKM2 expressed in E.coli (AA 141-248)
阳性对照	WB: K562, MCF7, HeLa, HepG2, A431, THP-1, Raji, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue; Human skin cancer tissue, Human prostate tissue, Human prostate cancer tissue, Human colorectal cancer tissue, Human liver cancer tissue. ICC/IF
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified and Protein A purified
物理外观	Liquid
储存缓冲液	10 mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂	0.05% Proclin 300
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

PKM Tchem 丙酮酸激酶PKM(Pyruvate kinase PKM) (0 活性数据)

点击查看靶蛋白信息： PKM

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - Western Blot
All lanes: PKM Antibody (Ab122143) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 1.6 s

IF/ICC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IF/ICC
FITC staining on IF
Samples: Human MCF7 cells
Primary Ab: 20 ug/mL PKM Antibody (Ab122143)
Second Ab: 1 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Skin cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Prostate Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

应用

应用名称	稀释比例
IHC	5-20 µg/mL
IF/ICC	5-20 µg/mL
WB	0.01-2 µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F1001236	分析证书	Ab122143
ZJ23F1001237	分析证书	Ab122143
ZJ24F0911332	分析证书	Ab122143

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

免疫肽封闭实验

Categories: 实验方案（Protocols）

探索主题:

多克隆抗体免疫肽封闭特异性结合

Immunopeptide blocking assay

Categories: 实验方案（Protocols）

探索主题:

Immunopeptideblocking PolyclonalAntibody SpecificBinding

Immunopeptide blocking assay

Categories: 实验方案（Protocols）

探索主题:

Immunopeptideblocking PolyclonalAntibody SpecificBinding

叠氮化钠去除实验

Categories: 实验方案（Protocols）

探索主题:

重组抗体叠氮化钠去除抗体纯化葡聚糖凝胶G-25 无叠氮化钠抗体

Sodium Azide Removal Protocol

Categories: 实验方案（Protocols）

探索主题:

Recombinant antibody Sodium Azide Removal Antibody Purification Sephadex G-25 Sodium Azide-Free Antibody

可替换产品

引用文献

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2. Takenaka M, Noguchi T, Sadahiro S, Hirai H, Yamada K, Matsuda T, Imai E, Tanaka T. (1991) Isolation and characterization of the human pyruvate kinase M gene.. Eur J Biochem, 198 (1): (101-6). [PMID:2040271]
3. Li J, Li S, Guo J, Li Q, Long J, Ma C, Ding Y, Yan C, Li L, Wu Z et al.. (2018) Natural Product Micheliolide (MCL) Irreversibly Activates Pyruvate Kinase M2 and Suppresses Leukemia.. J Med Chem, 61 (9): (4155-4164). [PMID:29641204]
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6. Ding Y, Xue Q, Liu S, Hu K, Wang D, Wang T, Li Y, Guo H, Hao X, Ge W et al.. (2020) Identification of Parthenolide Dimers as Activators of Pyruvate Kinase M2 in Xenografts of Glioblastoma Multiforme in Vivo.. J Med Chem, 63 (4): (1597-1611). [PMID:31977207]
7. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211]
8. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
9. Boxer MB, Jiang JK, Vander Heiden MG, Shen M, Skoumbourdis AP, Southall N, Veith H, Leister W, Austin CP, Park HW, Inglese J, Cantley LC, Auld DS, Thomas CJ.. (2010) Evaluation of substituted N,N'-diarylsulfonamides as activators of the tumor cell specific M2 isoform of pyruvate kinase.. J Med Chem, 53 (3): (1048-1055). [PMID:20017496] [10.1021/jm901577g]
10. Ning X, Li Y, Qi H, Li R, Jin Y, Liu J, Yin Y.. (2018) Anti-cancer effect of a novel 2,3-didithiocarbamate-substituted naphthoquinone as a tumor metabolic suppressor in vitro and in vivo.. Medchemcomm, 9 (4): (632-638). [PMID:30108954] [10.1039/C8MD00062J]
11. Asquith CRM, Naegeli KM, East MP, Laitinen T, Havener TM, Wells CI, Johnson GL, Drewry DH, Zuercher WJ, Morris DC.. (2019) Design of a Cyclin G Associated Kinase (GAK)/Epidermal Growth Factor Receptor (EGFR) Inhibitor Set to Interrogate the Relationship of EGFR and GAK in Chordoma.. J Med Chem, 62 (9): (4772-4778). [PMID:30973735] [10.1021/acs.jmedchem.9b00350]
12. Ashizawa, K K, McPhie, P P, Lin, K H KH and Cheng, S Y SY.. (1991) An in vitro novel mechanism of regulating the activity of pyruvate kinase M2 by thyroid hormone and fructose 1, 6-bisphosphate.. Biochemistry, (23): [PMID:1854723]
13. Kato, H H, Fukuda, T T, Parkison, C C, McPhie, P P and Cheng, S Y SY.. (1989) Cytosolic thyroid hormone-binding protein is a monomer of pyruvate kinase.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2813362]
14. Tani, K K and 7 more authors.. (1988) Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma.. Gene, (20): [PMID:2854097]
15. Williams, J M JM, Chen, G C GC, Zhu, L L and Rest, R F RF.. (1998) Using the yeast two-hybrid system to identify human epithelial cell proteins that bind gonococcal Opa proteins: intracellular gonococci bind pyruvate kinase via their Opa proteins and require host pyruvate for growth.. Molecular microbiology, [PMID:9466265]
16. Garcia-Gonzalo, Francesc R FR and 7 more authors.. (2003) Interaction between HERC1 and M2-type pyruvate kinase.. FEBS letters, (27): [PMID:12650930]
17. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801]
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19. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455]
20. Dombrauckas, Jill D JD, Santarsiero, Bernard D BD and Mesecar, Andrew D AD.. (2005) Structural basis for tumor pyruvate kinase M2 allosteric regulation and catalysis.. Biochemistry, (12): [PMID:15996096]
21. Li, Yunfeng Y and 8 more authors.. (2005) High glucose upregulates pantothenate kinase 4 (PanK4) and thus affects M2-type pyruvate kinase (Pkm2).. Molecular and cellular biochemistry, [PMID:16132722]
22. Giannakopoulos, Nadia V NV and 9 more authors.. (2005) Proteomic identification of proteins conjugated to ISG15 in mouse and human cells.. Biochemical and biophysical research communications, (21): [PMID:16139798]
23. Zody, Michael C MC and 68 more authors.. (2006) Analysis of the DNA sequence and duplication history of human chromosome 15.. Nature, (30): [PMID:16572171]
24. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243]
25. Steták, Attila A and 5 more authors.. (2007) Nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death.. Cancer research, (15): [PMID:17308100]
26. Zahedi, René P RP and 8 more authors.. (2008) Phosphoproteome of resting human platelets.. Journal of proteome research, [PMID:18088087]
27. Lee, Jungwoon J, Kim, Hye Kyoung HK, Han, Yong-Mahn YM and Kim, Jungho J.. (2008) Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription.. The international journal of biochemistry & cell biology, [PMID:18191611]
28. Shimada, Nobukazu N, Shinagawa, Toshie T and Ishii, Shunsuke S.. (2008) Modulation of M2-type pyruvate kinase activity by the cytoplasmic PML tumor suppressor protein.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:18298799]
29. Christofk, Heather R HR, Vander Heiden, Matthew G MG, Wu, Ning N, Asara, John M JM and Cantley, Lewis C LC.. (2008) Pyruvate kinase M2 is a phosphotyrosine-binding protein.. Nature, (13): [PMID:18337815]
30. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976]
31. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
32. Vander Heiden, Matthew G MG and 10 more authors.. (2010) Evidence for an alternative glycolytic pathway in rapidly proliferating cells.. Science (New York, N.Y.), (17): [PMID:20847263]
33. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
34. Chen, Nan N and 8 more authors.. (2011) The oxygen sensor PHD3 limits glycolysis under hypoxia via direct binding to pyruvate kinase.. Cell research, [PMID:21483450]
35. Luo, Weibo W and 8 more authors.. (2011) Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1.. Cell, (27): [PMID:21620138]
36. Lv, Lei L and 14 more authors.. (2011) Acetylation targets the M2 isoform of pyruvate kinase for degradation through chaperone-mediated autophagy and promotes tumor growth.. Molecular cell, (24): [PMID:21700219]
37. Yang, Weiwei W and 8 more authors.. (2011) Nuclear PKM2 regulates β-catenin transactivation upon EGFR activation.. Nature, (1): [PMID:22056988]
38. Gao, Xueliang X, Wang, Haizhen H, Yang, Jenny J JJ, Liu, Xiaowei X and Liu, Zhi-Ren ZR.. (2012) Pyruvate kinase M2 regulates gene transcription by acting as a protein kinase.. Molecular cell, (9): [PMID:22306293]
39. Yang, Weiwei W and 9 more authors.. (2012) PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis.. Cell, (17): [PMID:22901803]
40. Chaneton, Barbara B and 12 more authors.. (2012) Serine is a natural ligand and allosteric activator of pyruvate kinase M2.. Nature, (15): [PMID:23064226]
41. Morgan, Hugh P HP and 6 more authors.. (2013) M2 pyruvate kinase provides a mechanism for nutrient sensing and regulation of cell proliferation.. Proceedings of the National Academy of Sciences of the United States of America, (9): [PMID:23530218]
42. Lv, Lei L and 11 more authors.. (2013) Mitogenic and oncogenic stimulation of K433 acetylation promotes PKM2 protein kinase activity and nuclear localization.. Molecular cell, (7): [PMID:24120661]
43. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315]
44. Chen, Z Z and 10 more authors.. (2015) TRIM35 Interacts with pyruvate kinase isoform M2 to suppress the Warburg effect and tumorigenicity in hepatocellular carcinoma.. Oncogene, (23): [PMID:25263439]
45. Bhardwaj, Abhishek A and Das, Sanjeev S.. (2016) SIRT6 deacetylates PKM2 to suppress its nuclear localization and oncogenic functions.. Proceedings of the National Academy of Sciences of the United States of America, (2): [PMID:26787900]
46. Boeckel, Jes-Niels JN and 12 more authors.. (2016) JMJD8 Regulates Angiogenic Sprouting and Cellular Metabolism by Interacting With Pyruvate Kinase M2 in Endothelial Cells.. Arteriosclerosis, thrombosis, and vascular biology, [PMID:27199445]
47. Cen, Shuizhong and 14 more authors.. (2020) TRAF4 acts as a fate checkpoint to regulate the adipogenic differentiation of MSCs by activating PKM2.. EBioMedicine, [PMID:32268273]

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