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抗体
多克隆抗体
PGM1 Antibody

PGM1 Antibody

级别和纯度:

见COA

应用:

IF/ICC
IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Rabbit IgG
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货	价格	数量
Ab121491-10μl	10μl	现货
Ab121491-50μl	50μL	现货
Ab121491-100μl	100μL	现货
Ab121491-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: PGM1

Accession#:P36871 (1) Gene ID:5236 (1) PGM1 (2)

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main product photo

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基本信息

产品名称	PGM1 Antibody
别名	葡萄糖磷酸变位酶1多克隆抗体 \| 葡萄糖磷酸变位酶1抗体 \| PGM1抗体
英文别名	EC 5.4.2.2 \| Glucose phosphomutase 1 \| PGM 1 \| CDG1T antibody \| Glucose phosphomutase 1 antibody \| GSD14 antibody \| OTTHUMP00000010519 antibody \| OTTHUMP00000010520 antibody \| PGM 1 antibody \| PGM1 antibody \| PGM1_HUMAN antibody \| Phosphoglucomutase 1 ant
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属	兔(Rabbit)
特异性	PGM1
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	Recombinant PGM1 expressed in E.coli (AA 1-235).
阳性对照	WB: HeLa, HEK293, A431, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human cerebrum, liver, kidney, stomach, breast cancer, prostate tissues. IF/ICC: HeLa cells.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified + Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂	0.05% Proclin 300
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

PGM1 Tbio 磷酸葡糖变位酶-1(Phosphoglucomutase-1) (0 活性数据)

点击查看靶蛋白信息： PGM1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: PGM1 Antibody (Ab121491)

PGM1 Antibody (Ab121491) - Western Blot
All lanes: PGM1 Antibody (Ab121491) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 65.9 s

IHC: PGM1 Antibody (Ab121491)

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Samples: Human Cerebrum Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PGM1 Antibody (Ab121491)

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PGM1 Antibody (Ab121491)

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PGM1 Antibody (Ab121491)

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Samples: Human Stomach Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PGM1 Antibody (Ab121491)

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PGM1 Antibody (Ab121491)

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Samples: Human Prostate Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PGM1 Antibody (Ab121491)

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Sample: Human Liver Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PGM1 Antibody (Ab121491)

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Sample: Human Prostate Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PGM1 Antibody (Ab121491)

PGM1 Antibody (Ab121491) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 10 µg/mL PGM1 Antibody (Ab121491)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IF/ICC: PGM1 Antibody (Ab121491)

PGM1 Antibody (Ab121491) - IF/ICC
IF analysis of PGM1 Antibody (Ab121491) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PGM1 Antibody (Ab121491) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

应用

应用名称	稀释比例
IHC	5-20 µg/mL
WB	0.01-2 µg/mL
IF/ICC	2.5 µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0404498	分析证书	Ab121491
ZJ24F0404497	分析证书	Ab121491
ZJ24F0404496	分析证书	Ab121491

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

免疫肽封闭实验

Categories: 实验方案（Protocols）

探索主题:

多克隆抗体免疫肽封闭特异性结合

Immunopeptide blocking assay

Categories: 实验方案（Protocols）

探索主题:

Immunopeptideblocking PolyclonalAntibody SpecificBinding

Immunopeptide blocking assay

Categories: 实验方案（Protocols）

探索主题:

Immunopeptideblocking PolyclonalAntibody SpecificBinding

叠氮化钠去除实验

Categories: 实验方案（Protocols）

探索主题:

重组抗体叠氮化钠去除抗体纯化葡聚糖凝胶G-25 无叠氮化钠抗体

Sodium Azide Removal Protocol

Categories: 实验方案（Protocols）

探索主题:

Recombinant antibody Sodium Azide Removal Antibody Purification Sephadex G-25 Sodium Azide-Free Antibody

引用文献

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
2. Whitehouse, D B DB and 7 more authors.. (1992) Phosphoglucomutase 1: complete human and rabbit mRNA sequences and direct mapping of this highly polymorphic marker on human chromosome 1.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1530890]
3. Takahashi, N N and Neel, J V JV.. (1993) Intragenic recombination at the human phosphoglucomutase 1 locus: predictions fulfilled.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:7902567]
4. March, R E RE and 7 more authors.. (1993) The classical human phosphoglucomutase (PGM1) isozyme polymorphism is generated by intragenic recombination.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:7902568]
5. Putt, W W and 5 more authors.. (1993) Phosphoglucomutase 1: a gene with two promoters and a duplicated first exon.. The Biochemical journal, (1): [PMID:8257433]
6. Gururaj, Anupama A, Barnes, Christopher J CJ, Vadlamudi, Ratna K RK and Kumar, Rakesh R.. (2004) Regulation of phosphoglucomutase 1 phosphorylation and activity by a signaling kinase.. Oncogene, (21): [PMID:15378030]
7. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414]
8. Yu, Li-Rong LR and 5 more authors.. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.. Journal of proteome research, [PMID:17924679]
9. Stojkovic, Tanya T and 9 more authors.. (2009) Muscle glycogenosis due to phosphoglucomutase 1 deficiency.. The New England journal of medicine, (23): [PMID:19625727]
10. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
11. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
12. Timal, Sharita S and 18 more authors.. (2012) Gene identification in the congenital disorders of glycosylation type I by whole-exome sequencing.. Human molecular genetics, (1): [PMID:22492991]
13. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
14. Pérez, Belén B and 6 more authors.. (2013) A novel congenital disorder of glycosylation type without central nervous system involvement caused by mutations in the phosphoglucomutase 1 gene.. Journal of inherited metabolic disease, [PMID:22976764]
15. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
16. Tegtmeyer, Laura C LC and 49 more authors.. (2014) Multiple phenotypes in phosphoglucomutase 1 deficiency.. The New England journal of medicine, (6): [PMID:24499211]
17. Lee, Yingying Y, Stiers, Kyle M KM, Kain, Bailee N BN and Beamer, Lesa J LJ.. (2014) Compromised catalysis and potential folding defects in in vitro studies of missense mutants associated with hereditary phosphoglucomutase 1 deficiency.. The Journal of biological chemistry, (14): [PMID:25288802]
18. Stiers, Kyle M KM, Kain, Bailee N BN, Graham, Abigail C AC and Beamer, Lesa J LJ.. (2016) Induced Structural Disorder as a Molecular Mechanism for Enzyme Dysfunction in Phosphoglucomutase 1 Deficiency.. Journal of molecular biology, (24): [PMID:26972339]

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