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Peroxiredoxin 2/PRP Mouse mAb

    应用:
  • WB
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
  • 亚型: Mouse IgG1
  • 克隆号: C2
  • 偶联: Unconjugated
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库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab121321-10μl
10μl 现货 Stock Image
Ab121321-50μl
50μL 现货 Stock Image
Ab121321-100μl
100μL 期货 Stock Image
Ab121321-1ml
1ml 期货 Stock Image

基本信息

产品名称 Peroxiredoxin 2/PRP Mouse mAb
别名 过氧化还原酶2(PRDX2)单克隆抗体 | Peroxiredoxin 2/PRP抗体 | 过氧化还原酶2(PRDX2)单克隆抗体 | 过氧化还原酶2(PRDX2)单克隆抗体 | Peroxiredoxin 2/PRP抗体 | 过氧化物酶 2/PRP 小鼠单克隆抗体 | 过氧化物酶 2/PRP 小鼠单抗
英文别名 HEL-S-2a | NKEF-B | NKEFB | PRP | PRX2 | PRXII | PTX1 | TDPX1 | TPX1 | TSA | EC 1.11.1 | MGC4104 | Natural killer cell-enhancing factor B | natural killer-enhancing factor B | NKEFBNKEF-B | Peroxiredoxin 2 | peroxiredoxin-2 | PRDX2 | PRPTDPX1 | thiol-spec
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属 小鼠(Mouse)
特异性 Peroxiredoxin 2/PRP
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse)
免疫原 Recombinant PRDX2 expressed in E.coli (AA 2-198)
阳性对照 WB: SK-N-SH, HeLa, HEK293, MCF7, LNCaP and NIH/3T3 cell lysates.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 C2
Format Whole IgG
亚型 Mouse IgG1
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein A + Protein G purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂 0.05% Proclin 300
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

PRDX2 Tbio 过氧化物酶-2(Peroxiredoxin-2) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

Peroxiredoxin 2/PRP Mouse mAb (Ab121321) - Western Blot
All lanes: Peroxiredoxin 2/PRP Mouse mAb (Ab121321) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 11.3 s

应用

应用名称 稀释比例
WB

0.01-2 µg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到3个结果

批号(Lot Number) 证书类型 货号
ZJ24F0404486 分析证书 Ab121321
ZJ24F0404485 分析证书 Ab121321
ZJ24F0404484 分析证书 Ab121321

技术文档和文章

细胞内ELISA实验方案
In-cell ELISA protocol
In-cell ELISA protocol
蛋白质印迹的一抗选择指南
Guidelines of Primary Antibodies for Western Blotting
Guidelines of Primary Antibodies for Western Blotting
高分子量蛋白质印迹法
Protocol of Western Blotting For High Molecular Weights
Protocol of Western Blotting For High Molecular Weights
Protocol of Western Blotting For High Molecular Weights
Protocol of Western Blotting For High Molecular Weights
叠氮化钠去除实验
Sodium Azide Removal Protocol

引用文献

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
2. Rasmussen, H H HH and 5 more authors..  (1992)  Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes..  Electrophoresis,  [PMID:1286667]
3. Shau, H H, Butterfield, L H LH, Chiu, R R and Kim, A A..  (1994)  Cloning and sequence analysis of candidate human natural killer-enhancing factor genes..  Immunogenetics,  [PMID:8026862]
4. Lim, Y S YS, Cha, M K MK, Kim, H K HK and Kim, I H IH..  (1994)  The thiol-specific antioxidant protein from human brain: gene cloning and analysis of conserved cysteine regions..  Gene,  (25): [PMID:8144038]
5. Golaz, O O and 9 more authors..  (1993)  Plasma and red blood cell protein maps: update 1993..  Electrophoresis,  [PMID:8313871]
6. Cha, M K MK and Kim, I H IH..  (1995)  Thioredoxin-linked peroxidase from human red blood cell: evidence for the existence of thioredoxin and thioredoxin reductase in human red blood cell..  Biochemical and biophysical research communications,  (26): [PMID:8554614]
7. Ji, H H and 5 more authors.. A two-dimensional gel database of human colon carcinoma proteins..  Electrophoresis,  [PMID:9150948]
8. Schröder, E E and 5 more authors..  (2000)  Crystal structure of decameric 2-Cys peroxiredoxin from human erythrocytes at 1.7 A resolution..  Structure (London, England : 1993),  (15): [PMID:10873855]
9. Rabilloud, Thierry T and 8 more authors..  (2002)  Proteomics analysis of cellular response to oxidative stress. Evidence for in vivo overoxidation of peroxiredoxins at their active site..  The Journal of biological chemistry,  (31): [PMID:11904290]
10. Chevallet, Mireille M and 5 more authors..  (2003)  Regeneration of peroxiredoxins during recovery after oxidative stress: only some overoxidized peroxiredoxins can be reduced during recovery after oxidative stress..  The Journal of biological chemistry,  (26): [PMID:12853451]
11. Andersen, Jens S JS and 5 more authors..  (2003)  Proteomic characterization of the human centrosome by protein correlation profiling..  Nature,  (4): [PMID:14654843]
12. Gotter, Anthony L AL, Suppa, Christine C and Emanuel, Beverly S BS..  (2007)  Mammalian TIMELESS and Tipin are evolutionarily conserved replication fork-associated factors..  Journal of molecular biology,  (9): [PMID:17141802]
13. Rosenow, Anja A and 6 more authors..  (2012)  Resveratrol-induced changes of the human adipocyte secretion profile..  Journal of proteome research,  (7): [PMID:22905912]
14. Kamariah, Neelagandan; Sek, Mun Foong; Eisenhaber, Birgit; Eisenhaber, Frank and Grüber, Gerhard..  (2016)  Transition steps in peroxide reduction and a molecular switch for peroxide robustness of prokaryotic peroxiredoxins..  Scientific reports,  (28): [PMID:27892488]

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