Recombinant NXF1 Antibody

COA

级别和纯度:

重组

已验证

ExactAb™

0.3 mg/mL

应用:

IF/ICC
IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human)
亚型: Rabbit IgG
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab119077-10μl	10μl	现货
Ab119077-50μl	50μL	现货
Ab119077-100μl	100μL	期货
Ab119077-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: NXF1

Accession#:Q9UBU9 (1) Gene ID:10482 (1) NXF1 (1)

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基本信息

产品名称	Recombinant NXF1 Antibody
别名	核RNA输出因子1重组兔单克隆抗体 \| 核RNA输出因子1单克隆抗体 \| 核RNA输出因子1抗体 \| NXF1重组兔单克隆抗体 \| NXF1单克隆抗体 \| NXF1抗体 \| 核RNA输出因子1重组兔单克隆抗体 \| 核RNA输出因子1单克隆抗体 \| 核RNA输出因子1抗体 \| NXF1重组兔单克隆抗体 \| NXF1单克隆抗体 \| NXF1抗体 \| 重组 NXF1 抗体
英文别名	DKFZp667O0311 antibody \| DmNXF1 antibody \| MEX67 antibody \| MEX67, yeast, homolog of antibody \| Mex67p antibody \| mRNA export factor TAP antibody \| Mvb1 antibody \| Nuclear RNA export factor 1 antibody \| Nuclear RNA export factor 1 homolog (S. cerevisiae)
规格或纯度	ExactAb™, Validated, 重组, 0.3 mg/mL
宿主种属	兔(Rabbit)
特异性	NXF1
种属反应性	人(Human)
免疫原	A synthetic peptide derived from human NXF1 (AA 4-17)
阳性对照	WB: K562, HeLa, HEK293, Hep G2 and A549 cell lysates. IHC: Human breast cancer tissue. ICC/IF: Jurkat cells.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified
物理外观	Liquid
储存缓冲液	50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂	0.01% Sodium azide
浓度	0.3 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

NXF1 Tbio 核 RNA 输出因子 1(Nuclear RNA export factor 1) (0 活性数据)

点击查看靶蛋白信息： NXF1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: Recombinant NXF1 Antibody (Ab119077)

Recombinant NXF1 Antibody (Ab119077) - Western Blot
All lanes: Recombinant NXF1 Antibody (Ab119077) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 57.6 s

IF/ICC: Recombinant NXF1 Antibody (Ab119077)

Recombinant NXF1 Antibody (Ab119077) - IF/ICC
Immunocytochemistry analysis of NXF1 (green) in Jurkat using Recombinant NXF1 Antibody (Ab119077), and cells were counterstained with DAPI (blue).

IHC: Recombinant NXF1 Antibody (Ab119077)

Recombinant NXF1 Antibody (Ab119077) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant NXF1 Antibody (Ab119077). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

应用

应用名称	稀释比例
WB	1/500-1/1000
IHC	1/50 - 1/100
IF/ICC	1/50-1/200

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ24F0303464	分析证书	Ab119077
ZJ24F0303463	分析证书	Ab119077

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

免疫荧光染色实验步骤（甲醇通透法）

Categories: 实验方案（Protocols）

探索主题:

冷冻切片免疫荧光染色组织切片甲醇通透 IF

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

引用文献

1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
3. Yoon, D W DW and 5 more authors.. (1997) Tap: a novel cellular protein that interacts with tip of herpesvirus saimiri and induces lymphocyte aggregation.. Immunity, [PMID:9175835]
4. Grüter, P P and 8 more authors.. (1998) TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.. Molecular cell, [PMID:9660949]
5. Braun, I C IC, Rohrbach, E E, Schmitt, C C and Izaurralde, E E.. (1999) TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus.. The EMBO journal, (1): [PMID:10202158]
6. Katahira, J J and 5 more authors.. (1999) The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human.. The EMBO journal, (4): [PMID:10228171]
7. Kang, Y Y and Cullen, B R BR.. (1999) The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences.. Genes & development, (1): [PMID:10323864]
8. Bear, J J and 5 more authors.. (1999) Identification of novel import and export signals of human TAP, the protein that binds to the constitutive transport element of the type D retrovirus mRNAs.. Molecular and cellular biology, [PMID:10454577]
9. Bachi, A A and 10 more authors.. (2000) The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates.. RNA (New York, N.Y.), [PMID:10668806]
10. Tang, H H and Wong-Staal, F F.. (2000) Specific interaction between RNA helicase A and Tap, two cellular proteins that bind to the constitutive transport element of type D retrovirus.. The Journal of biological chemistry, (20): [PMID:10924507]
11. Liker, E E, Fernandez, E E, Izaurralde, E E and Conti, E E.. (2000) The structure of the mRNA export factor TAP reveals a cis arrangement of a non-canonical RNP domain and an LRR domain.. The EMBO journal, (1): [PMID:11060011]
12. Suyama, M M and 5 more authors.. (2000) Prediction of structural domains of TAP reveals details of its interaction with p15 and nucleoporins.. EMBO reports, [PMID:11256625]
13. Braun, I C IC, Herold, A A, Rode, M M, Conti, E E and Izaurralde, E E.. (2001) Overexpression of TAP/p15 heterodimers bypasses nuclear retention and stimulates nuclear mRNA export.. The Journal of biological chemistry, (8): [PMID:11259411]
14. Kim, V N VN, Kataoka, N N and Dreyfuss, G G.. (2001) Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex.. Science (New York, N.Y.), (7): [PMID:11546873]
15. Lykke-Andersen, J J, Shu, M D MD and Steitz, J A JA.. (2001) Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1.. Science (New York, N.Y.), (7): [PMID:11546874]
16. Fribourg, S S, Braun, I C IC, Izaurralde, E E and Conti, E E.. (2001) Structural basis for the recognition of a nucleoporin FG repeat by the NTF2-like domain of the TAP/p15 mRNA nuclear export factor.. Molecular cell, [PMID:11583626]
17. Kataoka, N N, Diem, M D MD, Kim, V N VN, Yong, J J and Dreyfuss, G G.. (2001) Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex.. The EMBO journal, (15): [PMID:11707413]
18. Grant, Richard P RP, Neuhaus, David D and Stewart, Murray M.. (2003) Structural basis for the interaction between the Tap/NXF1 UBA domain and FG nucleoporins at 1A resolution.. Journal of molecular biology, (21): [PMID:12581645]
19. Forler, Daniel D and 8 more authors.. (2004) RanBP2/Nup358 provides a major binding site for NXF1-p15 dimers at the nuclear pore complex and functions in nuclear mRNA export.. Molecular and cellular biology, [PMID:14729961]
20. Chan, Chia C CC and 6 more authors.. (2004) eIF4A3 is a novel component of the exon junction complex.. RNA (New York, N.Y.), [PMID:14730019]
21. Lindtner, Susan S and 9 more authors.. (2006) RNA-binding motif protein 15 binds to the RNA transport element RTE and provides a direct link to the NXF1 export pathway.. The Journal of biological chemistry, (1): [PMID:17001072]
22. Lai, Ming-Chih MC, Lee, Yan-Hwa Wu YH and Tarn, Woan-Yuh WY.. (2008) The DEAD-box RNA helicase DDX3 associates with export messenger ribonucleoproteins as well as tip-associated protein and participates in translational control.. Molecular biology of the cell, [PMID:18596238]
23. Kim, Miri M, Bellini, Michel M and Ceman, Stephanie S.. (2009) Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.. Molecular and cellular biology, [PMID:18936162]
24. Katahira, Jun J, Inoue, Hitomi H, Hurt, Ed E and Yoneda, Yoshihiro Y.. (2009) Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA.. The EMBO journal, (4): [PMID:19165146]
25. Johnson, Lisa A LA, Li, Ling L and Sandri-Goldin, Rozanne M RM.. (2009) The cellular RNA export receptor TAP/NXF1 is required for ICP27-mediated export of herpes simplex virus 1 RNA, but the TREX complex adaptor protein Aly/REF appears to be dispensable.. Journal of virology, [PMID:19369354]
26. Uranishi, Hiroaki H and 9 more authors.. (2009) The RNA-binding motif protein 15B (RBM15B/OTT3) acts as cofactor of the nuclear export receptor NXF1.. The Journal of biological chemistry, (18): [PMID:19586903]
27. Hautbergue, Guillaume M GM and 8 more authors.. (2009) UIF, a New mRNA export adaptor that works together with REF/ALY, requires FACT for recruitment to mRNA.. Current biology : CB, (1): [PMID:19836239]
28. Ruepp, Marc-David MD and 6 more authors.. (2009) Mammalian pre-mRNA 3' end processing factor CF I m 68 functions in mRNA export.. Molecular biology of the cell, [PMID:19864460]
29. Wickramasinghe, Vihandha O VO and 9 more authors.. (2010) mRNA export from mammalian cell nuclei is dependent on GANP.. Current biology : CB, (12): [PMID:20005110]
30. Sun, Chia-Yun Jessica CY and 10 more authors.. (2011) Facioscapulohumeral muscular dystrophy region gene 1 is a dynamic RNA-associated and actin-bundling protein.. Journal of molecular biology, (12): [PMID:21699900]
31. Viphakone, Nicolas N and 7 more authors.. (2012) TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export.. Nature communications, [PMID:22893130]
32. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
33. Chang, Chung-Te CT and 6 more authors.. (2013) Chtop is a component of the dynamic TREX mRNA export complex.. The EMBO journal, (6): [PMID:23299939]
34. Umlauf, David D and 8 more authors.. (2013) The human TREX-2 complex is stably associated with the nuclear pore basket.. Journal of cell science, (15): [PMID:23591820]
35. Teng, I-Fang IF and Wilson, Stuart A SA.. (2013) Mapping interactions between mRNA export factors in living cells.. PloS one, [PMID:23826332]
36. Viphakone, Nicolas N and 6 more authors.. (2015) Luzp4 defines a new mRNA export pathway in cancer cells.. Nucleic acids research, (27): [PMID:25662211]
37. Roundtree, Ian A IA and 12 more authors.. (2017) YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs.. eLife, (6): [PMID:28984244]

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所需的复溶浓度

Recombinant NXF1 Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器