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NKG2D Mouse mAb

    应用:
  • Flow Cytometry
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG1
  • 克隆号: 1D11
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab118154-10μg
10μg 现货 Stock Image
Ab118154-50μg
50μg 现货 Stock Image
Ab118154-100μg
100μg 现货 Stock Image
Ab118154-1mg
1mg 期货 Stock Image

基本信息

产品名称 NKG2D Mouse mAb
别名 NK细胞受体2D(CD314)抗体 | Anti-NKG2D抗体 | NKG2D抗体 | NKG2D 小鼠单克隆抗体 | NKG2D 小鼠单抗
英文别名 CD314 antigen | CD314 | D12S2489E | FLJ17759 | FLJ75772 | Killer cell lectin-like receptor subfamily K member 1 | killer cell lectin-like receptor subfamily K, member 1 | KLR | KLRK1 | NK cell receptor D | NKG2-D type II integral membrane protein | NKG2D
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属 小鼠(Mouse)
特异性 NKG2D
种属反应性 人(Human)
免疫原 NKL cells
阳性对照 Flow: Human peripheral blood lymphocytes.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 1D11
Format Whole IgG
亚型 Mouse IgG1
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein G purified
物理外观 Liquid
储存缓冲液 10mM PBS, pH 7.2-7.4
防腐剂 No
浓度 见COA
储存温度 2-8°C储存
运输条件 冰袋运输
稳定性与储存 4°C 下运输。4°C 长期保存(12 个月)。收货后建议分装。
分子类型 抗体

关联靶点(人)

CASC3 Tbio 蛋白质 CASC3(Protein CASC3) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
KLRK1 Tbio NKG2-D II 型整合膜蛋白(NKG2-D type II integral membrane protein) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

NKG2D Mouse mAb (Ab118154) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling NKG2D with NKG2D Mouse mAb (Ab118154) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150).

NKG2D Mouse mAb (Ab118154) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling NKG2D (red) with NKG2D Mouse mAb (Ab118154) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称 稀释比例
Flow Cytometry

1μg/100w cells

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ24F0505806 分析证书 Ab118154
ZJ24F0505807 分析证书 Ab118154
ZJ24F0505808 分析证书 Ab118154

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引用文献

1. Houchins JP, Yabe T, McSherry C, Bach FH.  (1991)  DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells..  J Exp Med,  173  (4): (1017-20).  [PMID:2007850]
2. Bauer S, Groh V, Wu J, Steinle A, Phillips JH, Lanier LL, Spies T. Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA..  Science,  [PMID:10426993]
3. Groh V, Rhinehart R, Randolph-Habecker J, Topp MS, Riddell SR, Spies T. Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells..  Nat Immunol,  [PMID:11224526]
4. Glienke, J J and 7 more authors..  (1998)  The genomic organization of NKG2C, E, F, and D receptor genes in the human natural killer gene complex..  Immunogenetics,  [PMID:9683661]
5. Wu, J J and 6 more authors..  (1999)  An activating immunoreceptor complex formed by NKG2D and DAP10..  Science (New York, N.Y.),  (30): [PMID:10426994]
6. Wu, J J, Cherwinski, H H, Spies, T T, Phillips, J H JH and Lanier, L L LL..  (2000)  DAP10 and DAP12 form distinct, but functionally cooperative, receptor complexes in natural killer cells..  The Journal of experimental medicine,  (2): [PMID:11015446]
7. Shum, Benny P BP and 8 more authors..  (2002)  Conservation and variation in human and common chimpanzee CD94 and NKG2 genes..  Journal of immunology (Baltimore, Md. : 1950),  (1): [PMID:11751968]
8. Radaev, S S, Rostro, B B, Brooks, A G AG, Colonna, M M and Sun, P D PD..  (2001)  Conformational plasticity revealed by the cocrystal structure of NKG2D and its class I MHC-like ligand ULBP3..  Immunity,  [PMID:11754823]
9. Sutherland, Claire L CL and 5 more authors..  (2002)  UL16-binding proteins, novel MHC class I-related proteins, bind to NKG2D and activate multiple signaling pathways in primary NK cells..  Journal of immunology (Baltimore, Md. : 1950),  (15): [PMID:11777960]
10. Vivier, Eric E, Tomasello, Elena E and Paul, Pascale P..  (2002)  Lymphocyte activation via NKG2D: towards a new paradigm in immune recognition?.  Current opinion in immunology,  [PMID:11973127]
11. McFarland, Benjamin J BJ, Kortemme, Tanja T, Yu, Shuyuarn F SF, Baker, David D and Strong, Roland K RK..  (2003)  Symmetry recognizing asymmetry: analysis of the interactions between the C-type lectin-like immunoreceptor NKG2D and MHC class I-like ligands..  Structure (London, England : 1993),  [PMID:12679019]
12. Bacon, Louise L and 5 more authors..  (2004)  Two human ULBP/RAET1 molecules with transmembrane regions are ligands for NKG2D..  Journal of immunology (Baltimore, Md. : 1950),  (15): [PMID:15240696]
13. Rosen, David B DB and 5 more authors..  (2004)  A Structural basis for the association of DAP12 with mouse, but not human, NKG2D..  Journal of immunology (Baltimore, Md. : 1950),  (15): [PMID:15294961]
14. Chan, Camie W CW and 11 more authors..  (2006)  Interferon-producing killer dendritic cells provide a link between innate and adaptive immunity..  Nature medicine,  [PMID:16444266]
15. Scherer, Steven E SE and 216 more authors..  (2006)  The finished DNA sequence of human chromosome 12..  Nature,  (16): [PMID:16541075]
16. Eagle, Robert A RA, Traherne, James A JA, Hair, James R JR, Jafferji, Insiya I and Trowsdale, John J..  (2009)  ULBP6/RAET1L is an additional human NKG2D ligand..  European journal of immunology,  [PMID:19658097]
17. Zafirova, Biljana; Wensveen, Felix M; Gulin, Maja and Polić, Bojan..  (2011)  Regulation of immune cell function and differentiation by the NKG2D receptor..  Cellular and molecular life sciences : CMLS,  [PMID:21898152]
18. Raulet, David H DH, Gasser, Stephan S, Gowen, Benjamin G BG, Deng, Weiwen W and Jung, Heiyoun H..  (2013)  Regulation of ligands for the NKG2D activating receptor..  Annual review of immunology,  [PMID:23298206]
19. Hosomi, Shuhei S and 11 more authors..  (2013)  CEACAM1 on activated NK cells inhibits NKG2D-mediated cytolytic function and signaling..  European journal of immunology,  [PMID:23696226]
20. Zuo, Jianmin J and 14 more authors..  (2017)  A disease-linked ULBP6 polymorphism inhibits NKG2D-mediated target cell killing by enhancing the stability of NKG2D ligand binding..  Science signaling,  (30): [PMID:28559451]

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