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NGF Mouse mAb

    应用:
  • WB
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human), 牛(Cow), 猪(Pig)
  • 亚型: Mouse IgG1
  • 克隆号: C2
  • 偶联: Unconjugated
有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab118002-10μl
10μl 现货 Stock Image
Ab118002-50μl
50μL 现货 Stock Image
Ab118002-100μl
100μL 现货 Stock Image
Ab118002-1ml
1ml 期货 Stock Image

基本信息

产品名称 NGF Mouse mAb
别名 神经生长因子β抗体 | NGF小鼠单克隆抗体 | NGF小鼠单抗
英文别名 Beta nerve growth factor precursor | beta-nerve growth factor | betaNGF | beta-NGF | HSAN5 | MGC161426 | nerve growth factor (beta polypeptide) | nerve growth factor, beta subunit | NGFB | NGFBMGC161428 | Beta nerve growth factor antibody | Beta NGF antib
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属 小鼠(Mouse)
特异性 NGF 
种属反应性 人(Human),牛(Cow),猪(Pig)
免疫原 Recombinant NGF expressed in E.coli (AA 19-239)
阳性对照 WB: HeLa, Jurkat, A549 and HEK293 cell lysates.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 C2
Format Whole IgG
亚型 Mouse IgG1
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein A + Protein G purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂 0.05% Proclin 300
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(4 周)。-20°C下长期保存(12个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

NGF Tchem β神经生长因子(Beta-nerve growth factor) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

NGF Mouse mAb (Ab118002) - Western Blot
All lanes: NGF Mouse mAb (Ab118002) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 28 kDa
Observed band size: 31 kDa
Exposure time: 109.0 s

应用

应用名称 稀释比例
WB

0.01-2 µg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到3个结果

批号(Lot Number) 证书类型 货号
ZJ23F1101688 分析证书 Ab118002
ZJ23F1101689 分析证书 Ab118002
ZJ24F1011910 分析证书 Ab118002

技术文档和文章

石蜡样品的免疫组化染色方案(IHC-P)
蛋白质印迹的一抗选择指南
Guidelines of Primary Antibodies for Western Blotting
Guidelines of Primary Antibodies for Western Blotting
高分子量蛋白质印迹法
Protocol of Western Blotting For High Molecular Weights
Protocol of Western Blotting For High Molecular Weights
Protocol of Western Blotting For High Molecular Weights
Protocol of Western Blotting For High Molecular Weights
叠氮化钠去除实验
Sodium Azide Removal Protocol

引用文献

1. Klein, R R, Jing, S Q SQ, Nanduri, V V, O'Rourke, E E and Barbacid, M M..  (1991)  The trk proto-oncogene encodes a receptor for nerve growth factor..  Cell,  (5): [PMID:1849459]
2. Hallböök, F F, Ibáñez, C F CF and Persson, H H..  (1991)  Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary..  Neuron,  [PMID:2025430]
3. Borsani, G G and 7 more authors..  (1990)  cDNA sequence of human beta-NGF..  Nucleic acids research,  (11): [PMID:2374737]
4. Ullrich, A A, Gray, A A, Berman, C C, Coussens, L L and Dull, T J TJ..  (1983)  Sequence homology of human and mouse beta-NGF subunit genes..  Cold Spring Harbor symposia on quantitative biology,  [PMID:6327169]
5. Ullrich, A A, Gray, A A, Berman, C C and Dull, T J TJ..  (1983)  Human beta-nerve growth factor gene sequence highly homologous to that of mouse..  Nature,  (30): [PMID:6688123]
6. Tong, Y Y, Wang, H H and Chen, W W..  (1997)  [Cloning and sequencing of the gene for premature beta nerve growth factor]..  Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology,  [PMID:10322959]
7. Cargill, M M and 17 more authors..  (1999)  Characterization of single-nucleotide polymorphisms in coding regions of human genes..  Nature genetics,  [PMID:10391209]
8. Culmsee, C C, Semkova, I I and Krieglstein, J J..  (1999)  NGF mediates the neuroprotective effect of the beta2-adrenoceptor agonist clenbuterol in vitro and in vivo: evidence from an NGF-antisense study..  Neurochemistry international,  [PMID:10403429]
9. Wiesmann, C C, Ultsch, M H MH, Bass, S H SH and de Vos, A M AM..  (1999)  Crystal structure of nerve growth factor in complex with the ligand-binding domain of the TrkA receptor..  Nature,  (9): [PMID:10490030]
10. Semkova, I I and Krieglstein, J J..  (1999)  Neuroprotection mediated via neurotrophic factors and induction of neurotrophic factors..  Brain research. Brain research reviews,  [PMID:10525174]
11. Puls, I I and 5 more authors..  (1999)  [Clenbuterol in amyotrophic lateral sclerosis. No indication for a positive effect]..  Der Nervenarzt,  [PMID:10637819]
12. Riaz, S S SS and Tomlinson, D R DR..  (1999)  Clenbuterol stimulates neurotrophic support in streptozotocin-diabetic rats..  Diabetes, obesity & metabolism,  [PMID:11221812]
13. Einarsdottir, Elisabet E and 8 more authors..  (2004)  A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception..  Human molecular genetics,  (15): [PMID:14976160]
14. He, Xiao-Lin XL and Garcia, K Christopher KC..  (2004)  Structure of nerve growth factor complexed with the shared neurotrophin receptor p75..  Science (New York, N.Y.),  (7): [PMID:15131306]
15. Gregory, S G SG and 178 more authors..  (2006)  The DNA sequence and biological annotation of human chromosome 1..  Nature,  (18): [PMID:16710414]
16. Wehrman, Tom T and 5 more authors..  (2007)  Structural and mechanistic insights into nerve growth factor interactions with the TrkA and p75 receptors..  Neuron,  (4): [PMID:17196528]
17. Wijeyewickrema, Lakshmi C LC, Gardiner, Elizabeth E EE, Gladigau, Elsa L EL, Berndt, Michael C MC and Andrews, Robert K RK..  (2010)  Nerve growth factor inhibits metalloproteinase-disintegrins and blocks ectodomain shedding of platelet glycoprotein VI..  The Journal of biological chemistry,  (16): [PMID:20164177]
18. Carvalho, Ofélia P OP and 8 more authors..  (2011)  A novel NGF mutation clarifies the molecular mechanism and extends the phenotypic spectrum of the HSAN5 neuropathy..  Journal of medical genetics,  [PMID:20978020]
19. Davidson, G L G and 20 more authors..  (2012)  Frequency of mutations in the genes associated with hereditary sensory and autonomic neuropathy in a UK cohort..  Journal of neurology,  [PMID:22302274]
20. Cao, Ziqin and 6 more authors..  (2020)  Targeting nerve growth factor, a new option for treatment of osteoarthritis: a network meta-analysis of comparative efficacy and safety with traditional drugs..  Aging,  (3): [PMID:33293475]

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