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Hsp40 Mouse mAb

    应用:
  • WB
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human), 小鼠(Mouse)
  • 亚型: Mouse IgG1
  • 克隆号: 5H5-D6-H3
  • 偶联: Unconjugated
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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab108854-10μl
10μl 现货 Stock Image
Ab108854-50μl
50μL 现货 Stock Image
Ab108854-100μl
100μL 期货 Stock Image
Ab108854-1ml
1ml 期货 Stock Image

基本信息

产品名称 Hsp40 Mouse mAb
别名 热休克蛋白家族40抗体 | Hsp40小鼠单克隆抗体 | Hsp40小鼠单抗
英文别名 DnaJ protein homolog 1 | hDj-1 | Heat shock 40 kDa protein 1 | Heat shock protein 40 | HSP40 | Human DnaJ protein 1 | DnaJ (Hsp40) homolog subfmaily B member 1 antibody | DNAJ 1 antibody | DNAJ B1 antibody | DnaJ heat shock protein family (Hsp40) member B
规格或纯度 ExactAb™, Validated, 1.0 mg/mL
宿主种属 小鼠(Mouse)
特异性 DNAJB1
种属反应性 人(Human),小鼠(Mouse)
免疫原 Recombinant Protein of Hsp40.
阳性对照 WB: Jurkat, HeLa, MCF7, Hep G2, A549, HEK293 and NIH/3T3 cell lysates.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 5H5-D6-H3
Format Whole IgG
亚型 Mouse IgG1
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein G purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.5% BSA, 0.02% Sodium azide, pH 7.3
防腐剂 0.02% Sodium azide
浓度 1.0 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

DNAJB1 Tbio DnaJ 同源物亚家族 B 成员 1(DnaJ homolog subfamily B member 1) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

Hsp40 Mouse mAb (Ab108854) - Western Blot
All lanes: Hsp40 Mouse mAb (Ab108854) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 38 kDa
Observed band size: 40 kDa
Exposure time: 36.9 s

应用

应用名称 稀释比例
WB

1/500-1/1000

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到2个结果

批号(Lot Number) 证书类型 货号
ZJ24F0505908 分析证书 Ab108854
ZJ24F0505909 分析证书 Ab108854

技术文档和文章

蛋白质印迹的一抗选择指南
Guidelines of Primary Antibodies for Western Blotting
Guidelines of Primary Antibodies for Western Blotting
高分子量蛋白质印迹法
Protocol of Western Blotting For High Molecular Weights
Protocol of Western Blotting For High Molecular Weights
Protocol of Western Blotting For High Molecular Weights
Protocol of Western Blotting For High Molecular Weights

引用文献

1. Rauch JN,Gestwicki JE. Binding of human nucleotide exchange factors to heat shock protein 70 (Hsp70) generates functionally distinct complexes in vitro..  [PMID:24318877]
2. Hattori, H H and 7 more authors..  (1992)  Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells..  Cell structure and function,  [PMID:1586970]
3. Raabe, T T and Manley, J L JL..  (1991)  A human homologue of the Escherichia coli DnaJ heat-shock protein..  Nucleic acids research,  (11): [PMID:1754405]
4. and Ohtsuka, K K..  (1993)  Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ..  Biochemical and biophysical research communications,  (30): [PMID:8250930]
5. Qian, Y Q YQ, Patel, D D, Hartl, F U FU and McColl, D J DJ..  (1996)  Nuclear magnetic resonance solution structure of the human Hsp40 (HDJ-1) J-domain..  Journal of molecular biology,  (12): [PMID:8764402]
6. Hata, M M, Okumura, K K, Seto, M M and Ohtsuka, K K..  (1996)  Genomic cloning of a human heat shock protein 40 (Hsp40) gene (HSPF1) and its chromosomal localization to 19p13.2..  Genomics,  (15): [PMID:8975727]
7. Shi, Y Y, Mosser, D D DD and Morimoto, R I RI..  (1998)  Molecular chaperones as HSF1-specific transcriptional repressors..  Genes & development,  (1): [PMID:9499401]
8. Melville, M W MW and 5 more authors..  (1999)  The cellular inhibitor of the PKR protein kinase, P58(IPK), is an influenza virus-activated co-chaperone that modulates heat shock protein 70 activity..  The Journal of biological chemistry,  (5): [PMID:9920933]
9. Grimwood, Jane J and 97 more authors..  (2004)  The DNA sequence and biology of human chromosome 19..  Nature,  (1): [PMID:15057824]
10. Van Damme, Petra P and 15 more authors..  (2012)  N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB..  Proceedings of the National Academy of Sciences of the United States of America,  (31): [PMID:22814378]
11. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,  (4): [PMID:23186163]
12. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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