货号 (SKU)

包装规格

是否现货

价格

数量

Ab107012-10μl

10μl

现货

Ab107012-50μl

50μL

现货

Ab107012-100μl

100μL

现货

Ab107012-1ml

1ml

期货

基本信息

产品名称	Recombinant HDAC1 Antibody
别名	组蛋白去乙酰化酶1(内参)重组单克隆抗体 \| 组蛋白去乙酰化酶1重组单克隆抗体 \| 组蛋白去乙酰化酶1抗体 \| 抗HDAC1抗体
英文别名	DKFZp686H12203 antibody \| GON 10 antibody \| HD1 antibody \| HDAC 1 antibody \| HDAC1 antibody \| HDAC1_HUMAN antibody \| Histone deacetylase 1 antibody \| Reduced potassium dependency yeast homolog like 1 antibody \| RPD3 antibody \| RPD3L1 antibody \| HDAC1 \| GO
规格或纯度	ExactAb™, Validated, Recombinant, High performance, 0.125 mg/mL
宿主种属	兔(Rabbit)
特异性	HDAC1
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse),牛(Cow),绿猴(Green Monkey)
交叉反应	Cross-reactivity with other species has not been measured.
免疫原	A synthetic peptide corresponding to the C-term of HDAC1 was used as an immunogen.
阳性对照	WB: Jurkat, K562, MOLT-4, HeLa, THP-1, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human colon tissue. Flow: HeLa cells. IF/ICC: HeLa cells.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
克隆号	RR672
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3
防腐剂	0.01% Sodium azide
浓度	0.125 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

HDAC1 Tclin 组蛋白去乙酰化酶1(Histone deacetylase 1) (0 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

HDAC1 Tclin Histone deacetylase 1 (10854 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HDAC1 Tclin Histone deacetylase (6747 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HDAC1 Tclin Histone deacetylase (HDAC1 and HDAC2) (618 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HDAC1 Tclin Histone deacetylase 1/3/5/8 (49 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HDAC1 Tclin Histone deacetylase 1/2/3/6 (89 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HDAC1 Tclin Histone deacetylase 1/2/3 (160 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HDAC1 Tclin Histone deacetylase 1/8 (2 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HDAC1 Tclin Histone deacetylase 1/Nuclear receptor corepressor 2 (6 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HDAC1 Tclin BRD4/HDAC1 (6 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HDAC1 Tclin Histone deacetylase 1/6 (127 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HDAC1 Tclin HDAC1/REST corepressor 3 (5 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HDAC1 Tclin Cereblon/Histone deacetylase 1 (11 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

HDAC1 Tclin Class 1 histone deacetylase (459 活性数据)

点击查看靶蛋白信息： HDAC1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Recombinant HDAC1 Antibody (Ab107012)

Recombinant HDAC1 Antibody (Ab107012) - Western Blot
All lanes: Recombinant HDAC1 Antibody (Ab107012) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 55 kDa
Observed band size: 61 kDa
Exposure time: 10.0 s

Western Blot: Recombinant HDAC1 Antibody (Ab107012)

IF/ICC: Recombinant HDAC1 Antibody (Ab107012)

Recombinant HDAC1 Antibody (Ab107012) - IF/ICC
IF analysis of HDAC1 (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant HDAC1 Antibody (Ab107012) at 0.25 μg/mL in blocking buffer overnight at 4 °C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

IF/ICC: Recombinant HDAC1 Antibody (Ab107012)

Recombinant HDAC1 Antibody (Ab107012) - IF/ICC
Recombinant HDAC1 Antibody (Ab107012) staining HDAC1 in Hela cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were
fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant HDAC1 Antibody (Ab107012) (1/500) at 4°C. An AF594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AF594-conjugated Goat Anti-Rabbit IgG (1/500).

IHC: Recombinant HDAC1 Antibody (Ab107012)

Recombinant HDAC1 Antibody (Ab107012) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human colon tissue labelling HDAC1 with Recombinant HDAC1 Antibody (Ab107012) at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Flow Cytometry: Recombinant HDAC1 Antibody (Ab107012) Flow Cytometry: Recombinant HDAC1 Antibody (Ab107012)

Recombinant HDAC1 Antibody (Ab107012) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant HDAC1 Antibody (Ab107012) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant HDAC1 Antibody (Ab107012) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

应用

应用名称	稀释比例
IHC	1/100 - 1/200
WB	1/1000 - 1/2000
Flow Cytometry	1/50 - 1/200
IF/ICC	1/200 - 1/500

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F1001411	分析证书	Ab107012
ZJ23F1001410	分析证书	Ab107012
ZJ24F1011839	分析证书	Ab107012

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