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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab105966-10μl |
10μl |
现货 ![]() |
| |
| Ab105966-50μl |
50μL |
现货 ![]() |
| |
| Ab105966-100μl |
100μL |
期货 ![]() |
| |
| Ab105966-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | GNMT Mouse mAb |
|---|---|
| 别名 | 甘氨酸-N-甲基转移酶抗体 |
| 英文别名 | EC 2.1.1.20 antibody | Epididymis secretory sperm binding protein Li 182mP antibody | Glycine N methyltransferase antibody | Glycine N-methyltransferase antibody | Gnmt antibody | GNMT_HUMAN antibody | HEL S 182mP antibody | OTTHUMP00000016412 antibody | |
| 规格或纯度 | ExactAb™, 已验证, 无载体, 0.5 mg/mL |
| 生化机理 | 利用 S-腺苷蛋氨酸(AdoMet)催化甘氨酸甲基化,形成 N-甲基甘氨酸(肌氨酸),同时产生 S-腺苷高半胱氨酸(AdoHcy)。可能在调节组织中 AdoMet 的浓度和蛋氨酸的代谢中发挥关键作用。 |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | GNMT |
| 种属反应性 | 人(Human),小鼠(Mouse) |
| 免疫原 | Recombinant protein of human GNMT. |
| 阳性对照 | WB: Mouse liver lysates. Flow: U-2 OS cells. |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| Format | Whole IgG |
| 亚型 | Mouse IgG2a |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein G purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 30% Glycerol, 0.01% Sodium azide, pH 7.3 |
| 防腐剂 | 0.01% Sodium azide |
| 浓度 | 0.5 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4℃ 短期储存(1-2 周)。-20℃长期保存(12 个月)。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
GNMT Mouse mAb (Ab105966) - Western Blot
All lanes: GNMT Mouse mAb (Ab105966) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 31 kDa
Exposure time: 20.8 s
GNMT Mouse mAb (Ab105966) - Flow Cytometry
Flow cytometric analysis of U-2 OS cells using GNMT Mouse mAb (Ab105966). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37℃ for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37℃ for 40 min. Isotype control antibody (blue line) was used under the same condition.
| 应用名称 | 稀释比例 |
|---|---|
| WB | 1/500-1/2000 |
| Flow Cytometry | 1/10-1/50 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab105966 | |
| 分析证书 | Ab105966 |