Recombinant GAPDH Antibody

COA

级别和纯度:

见COA

应用:

IF/ICC
IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse), 猴(Monkey)
亚型: Rabbit IgG
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab104781-10μl	10μl	现货
Ab104781-50μl	50μL	期货
Ab104781-100μl	100μL	现货
Ab104781-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: GAPDH

Accession#:P04406 (14) GAPDH (14) Gene ID:2597 (14) Loading control (34)

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基本信息

产品名称	Recombinant GAPDH Antibody
别名	3-磷酸甘油醛脱氢酶内参抗体 \| 3-磷酸甘油醛脱氢酶抗体 \| 3-磷酸甘油醛脱氢酶单克隆抗体 \| 3-磷酸甘油醛脱氢酶重组单克隆抗体 \| GAPDH抗体 \| GAPDH单克隆抗体 \| GAPDH重组单克隆抗体
英文别名	38 kDa BFA-dependent ADP-ribosylation substrate antibody \| aging associated gene 9 protein antibody \| Aging-associated gene 9 proteinantibody \| BARS-38 antibody \| cb609 antibody \| EC 1.2.1.12antibody \| Epididymis secretory sperm binding protein Li 162eP a
规格或纯度	ExactAb™, 已验证, 重组, 高性能, 见COA
生化机理	Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Post-translational: S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus. ISGylated.
宿主种属	兔(Rabbit)
特异性	GAPDH
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse),猴(Monkey)
免疫原	A synthetic peptide derived from human GAPDH (AA 1-150).
阳性对照	WB: HeLa, A431, A549, MCF7, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human bladder cancer tissue. IF/ICC: HeLa cells.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4.
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
分子类型	抗体

关联靶点（人）

GAPDH Tchem 3-磷酸甘油醛脱氢酶(Glyceraldehyde-3-phosphate dehydrogenase) (0 活性数据)

点击查看靶蛋白信息： GAPDH

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: Recombinant GAPDH Antibody (Ab104781)

Recombinant GAPDH Antibody (Ab104781) - Western Blot
All lanes: Recombinant GAPDH Antibody (Ab104781) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 21.9 s

IHC: Recombinant GAPDH Antibody (Ab104781)

Recombinant GAPDH Antibody (Ab104781) - IHC
Immunohistochemical analysis of paraffin-embedded human bladder cancer,
using Recombinant GAPDH Antibody (Ab104781).

IF/ICC: Recombinant GAPDH Antibody (Ab104781)

Recombinant GAPDH Antibody (Ab104781) - IF/ICC
Immunofluorescent analysis of HeLa using the Recombinant GAPDH Antibody (Ab104781) at 1/50 dilution.

IF/ICC: Recombinant GAPDH Antibody (Ab104781)

Recombinant GAPDH Antibody (Ab104781) - IF/ICC
Immunofluorescent analysis of HeLa using the Recombinant GAPDH Antibody (Ab104781) at 1/50 dilution.