GAPDH Mouse mAb

COA

级别和纯度:

1.0 mg/mL

应用:

Flow Cytometry
IF/ICC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: B08/4A11
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab155825-10μl	10μl	现货
Ab155825-50μl	50μL	现货
Ab155825-100μl	100μL	现货
Ab155825-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: GAPDH

Accession#:P04406 (14) GAPDH (14) Gene ID:2597 (14) 流式细胞术 (1163)

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基本信息

产品名称	GAPDH Mouse mAb
别名	重组Anti-GAPDH抗体 \| 小鼠单克隆抗体 to GAPDH \| GAPDH小鼠单克隆抗体 \| GAPDH小鼠单抗
英文别名	38 kDa BFA-dependent ADP-ribosylation substrate antibody \| aging associated gene 9 protein antibody \| Aging-associated gene 9 protein antibody \| BARS-38 antibody \| cb609 antibody \| EC 1.2.1.12 antibody \| Epididymis secretory sperm binding protein Li 162eP
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 1.0 mg/mL
宿主种属	小鼠(Mouse)
特异性	GAPDH
种属反应性	人(Human)
免疫原	Recombinant full length human GAPDH
阳性对照	WB: HEK293, HeLa, Jurkat, A431, HepG2 and K562 whole cell lysates; Flow: Jurkat cells; ICC/IF: HeLa cells
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	B08/4A11
Format	Whole IgG
亚型	IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	PBS, pH 7.2
防腐剂	No
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 +4°C 下短期储存（1-2 周）。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

GAPDH Tchem 3-磷酸甘油醛脱氢酶(Glyceraldehyde-3-phosphate dehydrogenase) (0 活性数据)

点击查看靶蛋白信息： GAPDH

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: GAPDH Mouse mAb (Ab155825)

GAPDH Mouse mAb (Ab155825) - Western Blot
All lanes: GAPDH Antibody (Ab155825) at 0.05 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 35.8 kDa
Observed band size: 35.6 kDa
Exposure time: 36.9 s

Flow Cytometry: GAPDH Mouse mAb (Ab155825)

GAPDH Mouse mAb (Ab155825) - Flow Cytometry
Overlay histogram showing Jurkat cells stained with GAPDH Mouse mAb (Ab155825) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control.

ICC/IF: GAPDH Mouse mAb (Ab155825)

GAPDH Mouse mAb (Ab155825) - ICC/IF
IF analysis of GAPDH (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with GAPDH Mouse mAb (Ab155825) at 0.3μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

ICC/IF: GAPDH Mouse mAb (Ab155825)

GAPDH Mouse mAb (Ab155825) - ICC/IF
IF analysis of GAPDH (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with GAPDH Mouse mAb (Ab155825) at 0.3μg/mL and Recombinant COX IV Antibody (Ab097410) (Red) at 1μg/mL in blocking buffer for 2h at room temperature and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 and Goat Anti-Rabbit IgG H&L (Cy5) (Ab170147) for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

应用

应用名称	稀释比例
IF/ICC	0.5 μg/mL
WB	1/1000-1/100000 ; Predicted molecular weight: 37 kDa
Flow Cytometry	0.016 μg/mL- 1 μg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到7个结果

批号(Lot Number)	证书类型	货号
ZJ25F0926563	分析证书	Ab155825
ZJ25F0926562	分析证书	Ab155825
ZJ24F0910108	分析证书	Ab155825
ZJ24F0910107	分析证书	Ab155825
ZJ24F0404297	分析证书	Ab155825
ZJ23F1001304	分析证书	Ab155825
ZJ23F1001305	分析证书	Ab155825

技术文档和文章

免疫荧光染色实验步骤（甲醇通透法）

Categories: 实验方案（Protocols）

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冷冻切片免疫荧光染色组织切片甲醇通透 IF

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

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Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

利用甲醇通透法进行流式细胞术

Categories: 实验方案（Protocols）

探索主题:

流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

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Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

叠氮化钠去除实验

Categories: 实验方案（Protocols）

探索主题:

重组抗体叠氮化钠去除抗体纯化葡聚糖凝胶G-25 无叠氮化钠抗体

Sodium Azide Removal Protocol

Categories: 实验方案（Protocols）

探索主题:

Recombinant antibody Sodium Azide Removal Antibody Purification Sephadex G-25 Sodium Azide-Free Antibody

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引用文献

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溶液计算器

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计算溶液所需的质量、体积或浓度。

质量

浓度

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g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

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复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

GAPDH Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器