JavaScript 似乎在您的浏览器中禁用。 在我们的网站上获得最佳体验，请务必打开 Javascript 在您的浏览器。

This is a demo store. No orders will be fulfilled.

྾

首页
抗体
多克隆抗体
Galectin 3 Antibody

Galectin 3 Antibody

级别和纯度:

见COA

应用:

IF/ICC
IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse), 猪(Pig)
亚型: Rabbit IgG
偶联: Unconjugated

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
Ab104584-10μl	10μl	现货
Ab104584-50μl	50μL	现货
Ab104584-100μl	100μL	现货
Ab104584-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: LGALS3

Accession#:P17931 (7) Galectin 3 (6) Gene ID:3958 (7) LGALS3 (7)

Skip to the end of the images gallery

main product photo

Skip to the beginning of the images gallery

基本信息

产品名称	Galectin 3 Antibody
别名	anti-GAL3抗体 \| 半乳糖凝集素3多克隆抗体 \| 半乳糖凝集素3抗体
英文别名	35 kDa lectin antibody \| Carbohydrate binding protein 35 antibody \| Carbohydrate-binding protein 35 antibody \| CBP 35 antibody \| CBP35 antibody \| Gal-3 antibody \| GAL3 antibody \| Galactose-specific lectin 3 antibody \| Galactoside-binding protein antibody
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属	兔(Rabbit)
特异性	Galectin 3
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse),猪(Pig)
免疫原	Recombinant Galectin 3 expressed in E.coli (AA 1-250)
阳性对照	WB: C6, NIH/3T3, Hep G2, A549, HeLa, MCF7 and A431 cell lysates. IHC: Human colorectal cancer, lung cancer, ovarian cancer tissues; Porcine small intestine, kidney and spleen tissues. IHC: MCF7 cells.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified and Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂	0.05% Proclin 300
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

LGALS3 Tchem 半乳糖凝集素-3(Galectin-3) (0 活性数据)

点击查看靶蛋白信息： LGALS3

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Galectin 3 Antibody (Ab104584)

Galectin 3 Antibody (Ab104584) - Western Blot
All lanes: Galectin 3 Antibody (Ab104584) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 28 kDa
Observed band size: 29 kDa
Exposure time: 0.1 s

IF: Galectin 3 Antibody (Ab104584)

Galectin 3 Antibody (Ab104584) - IF
FITC staining on IF
Samples: Human MCF7 cells
Primary Ab: 20 μg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 1 μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 3 Antibody (Ab104584)

Galectin 3 Antibody (Ab104584) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 10 μg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 3 Antibody (Ab104584)

Galectin 3 Antibody (Ab104584) - IHC
DAB staining on IHC
Samples: Human Lung cancer Tissue
Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 3 Antibody (Ab104584)

Galectin 3 Antibody (Ab104584) - IHC
DAB staining on IHC
Samples: Human Ovarian cancer Tissue
Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 3 Antibody (Ab104584)

Galectin 3 Antibody (Ab104584) - IHC
DAB staining on IHC
Sample: Porcine Small intestine Tissue
Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 3 Antibody (Ab104584)

Galectin 3 Antibody (Ab104584) - IHC
DAB staining on IHC
Sample: Porcine Kidney Tissue
Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 3 Antibody (Ab104584)

Galectin 3 Antibody (Ab104584) - IHC
DAB staining on IHC
Sample: Porcine Spleen Tissue
Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

应用

应用名称	稀释比例
IHC	5-20 µg/mL
IF/ICC	5-20 µg/mL
WB	0.1-2 µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F1001225	分析证书	Ab104584
ZJ23F1001224	分析证书	Ab104584
ZJ24F0911306	分析证书	Ab104584

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

免疫肽封闭实验

Categories: 实验方案（Protocols）

探索主题:

多克隆抗体免疫肽封闭特异性结合

Immunopeptide blocking assay

Categories: 实验方案（Protocols）

探索主题:

Immunopeptideblocking PolyclonalAntibody SpecificBinding

Immunopeptide blocking assay

Categories: 实验方案（Protocols）

探索主题:

Immunopeptideblocking PolyclonalAntibody SpecificBinding

叠氮化钠去除实验

Categories: 实验方案（Protocols）

探索主题:

重组抗体叠氮化钠去除抗体纯化葡聚糖凝胶G-25 无叠氮化钠抗体

Sodium Azide Removal Protocol

Categories: 实验方案（Protocols）

探索主题:

Recombinant antibody Sodium Azide Removal Antibody Purification Sephadex G-25 Sodium Azide-Free Antibody

引用文献

1. Oberg CT, Blanchard H, Leffler H, Nilsson UJ.. (2008) Protein subtype-targeting through ligand epimerization: talose-selectivity of galectin-4 and galectin-8.. Bioorg Med Chem Lett, 18 (13): (3691-3694). [PMID:18539029] [10.1016/j.bmcl.2008.05.066]
2. Delaine T, Cumpstey I, Ingrassia L, Le Mercier M, Okechukwu P, Leffler H, Kiss R, Nilsson UJ.. (2008) Galectin-inhibitory thiodigalactoside ester derivatives have antimigratory effects in cultured lung and prostate cancer cells.. J Med Chem, 51 (24): (8109-8114). [PMID:19053747] [10.1021/jm801077j]
3. Rajput VK, MacKinnon A, Mandal S, Collins P, Blanchard H, Leffler H, Sethi T, Schambye H, Mukhopadhyay B, Nilsson UJ.. (2016) A Selective Galactose-Coumarin-Derived Galectin-3 Inhibitor Demonstrates Involvement of Galectin-3-glycan Interactions in a Pulmonary Fibrosis Model.. J Med Chem, 59 (17): (8141-8147). [PMID:27500311] [10.1021/acs.jmedchem.6b00957]
4. Gabr M,Rehman AU,Chen HF. (2020) Quinoline-Pyrazole Scaffold as a Novel Ligand of Galectin-3 and Suppressor of TREM2 Signaling.. ACS Med Chem Lett, 11 (9): (1759-1765). [PMID:32944144] [10.1021/acsmedchemlett.0c00330]
5. Raz, A A and 5 more authors.. (1991) Molecular cloning and chromosomal mapping of a human galactoside-binding protein.. Cancer research, (15): [PMID:2009535]
6. Oda, Y Y, Leffler, H H, Sakakura, Y Y, Kasai, K K and Barondes, S H SH.. (1991) Human breast carcinoma cDNA encoding a galactoside-binding lectin homologous to mouse Mac-2 antigen.. Gene, (15): [PMID:2022338]
7. Robertson, M W MW, Albrandt, K K, Keller, D D and Liu, F T FT.. (1990) Human IgE-binding protein: a soluble lectin exhibiting a highly conserved interspecies sequence and differential recognition of IgE glycoforms.. Biochemistry, (4): [PMID:2261464]
8. Cherayil, B J BJ, Chaitovitz, S S, Wong, C C and Pillai, S S.. (1990) Molecular cloning of a human macrophage lectin specific for galactose.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2402511]
9. Lotz, M M MM and 6 more authors.. (1993) Decreased expression of Mac-2 (carbohydrate binding protein 35) and loss of its nuclear localization are associated with the neoplastic progression of colon carcinoma.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:7682704]
10. Huflejt, M E ME, Turck, C W CW, Lindstedt, R R, Barondes, S H SH and Leffler, H H.. (1993) L-29, a soluble lactose-binding lectin, is phosphorylated on serine 6 and serine 12 in vivo and by casein kinase I.. The Journal of biological chemistry, (15): [PMID:8253806]
11. Kadrofske, M M MM, Openo, K P KP and Wang, J L JL.. (1998) The human LGALS3 (galectin-3) gene: determination of the gene structure and functional characterization of the promoter.. Archives of biochemistry and biophysics, (1): [PMID:9439577]
12. Sasaki, T T, Brakebusch, C C, Engel, J J and Timpl, R R.. (1998) Mac-2 binding protein is a cell-adhesive protein of the extracellular matrix which self-assembles into ring-like structures and binds beta1 integrins, collagens and fibronectin.. The EMBO journal, (16): [PMID:9501082]
13. Seetharaman, J J and 5 more authors.. (1998) X-ray crystal structure of the human galectin-3 carbohydrate recognition domain at 2.1-A resolution.. The Journal of biological chemistry, (22): [PMID:9582341]
14. Heilig, Roland R and 98 more authors.. (2003) The DNA sequence and analysis of human chromosome 14.. Nature, (6): [PMID:12508121]
15. Fukushi, Jun-ichi J, Makagiansar, Irwan T IT and Stallcup, William B WB.. (2004) NG2 proteoglycan promotes endothelial cell motility and angiogenesis via engagement of galectin-3 and alpha3beta1 integrin.. Molecular biology of the cell, [PMID:15181153]
16. Seidel, Ronald D RD, Zhuang, Tiandi T and Prestegard, James H JH.. (2007) Bound-state residual dipolar couplings for rapidly exchanging ligands of His-tagged proteins.. Journal of the American Chemical Society, (18): [PMID:17385862]
17. Than, Nandor Gabor NG and 24 more authors.. (2009) A primate subfamily of galectins expressed at the maternal-fetal interface that promote immune cell death.. Proceedings of the National Academy of Sciences of the United States of America, (16): [PMID:19497882]
18. Haudek, Kevin C and 5 more authors.. (2010) Dynamics of galectin-3 in the nucleus and cytoplasm.. Biochimica et biophysica acta, [PMID:19616076]
19. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
20. Escoda-Ferran, Cristina C and 8 more authors.. (2014) Modulation of CD6 function through interaction with Galectin-1 and -3.. FEBS letters, (25): [PMID:24945728]
21. Chauhan, Santosh S and 11 more authors.. (2016) TRIMs and Galectins Globally Cooperate and TRIM16 and Galectin-3 Co-direct Autophagy in Endomembrane Damage Homeostasis.. Developmental cell, (10): [PMID:27693506]
22. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

=

浓度

x

体积

x

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

x

体积 1 (V1)

=

浓度 2 (C2)

x

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

=

质量（小瓶）

÷

所需的复溶浓度

上海阿拉丁生化科技股份有限公司

销售条款及条件隐私政策

© 2016 ALADDIN-E.COM . 版权所有,未经授权禁止拷贝本站资料,如有违反,将追究法律责任

沪公网安备 31012002004058号沪ICP备09093490号-2

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。

危险品化学品经营许可证（带存储）

营业执照(三证合一)