DRP1 Mouse mAb

COA

级别和纯度:

见COA

应用:

IF/ICC
IHC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Mouse IgG2b
克隆号: OTI3E11
偶联: Unconjugated

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
Ab100522-10μl	10μl	期货
Ab100522-50μl	50μL	期货
Ab100522-100μl	100μL	期货
Ab100522-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: DNM1L

Accession#:O00429 (1) DNM1L (1) DRP1 (2) Gene ID:10059 (1)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本信息

产品名称	DRP1 Mouse mAb
别名	动力相关蛋白1多克隆抗体 \| 动力相关蛋白1抗体 \| 动力相关蛋白1单克隆抗体 \| DRP1抗体 \| DRP1单克隆抗体 \| DNM1L抗体 \| DNM1L单克隆抗体
英文别名	DLP1 antibody \| dnm1l antibody \| DNM1L_HUMAN antibody \| Dnm1p/Vps1p-like protein antibody \| dnml1 antibody \| DRP1 antibody \| DVLP antibody \| Dymple antibody \| Dynamin 1 like antibody \| Dynamin family member proline-rich carboxyl-terminal domain less antib
规格或纯度	见COA
生化机理	Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport. Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed. Post-translational: Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 also promotes mitochondrial fission. Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity. S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage. Ubiquitination by MARCH5 affects mitochondrial morphology.
宿主种属	小鼠(Mouse)
特异性	DRP1
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	Recombinant protein of human DNM1L (AA 1-736).
阳性对照	WB: HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 lysates. IHC: Human kidney, ovary adenocarcinoma, bladder, bladder carcinoma tissues. IF/ICC: COS7 cells.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Protein A/G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 1% BSA, 0.02% Sodium azide, pH 7.3
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	短期储存2-8℃（1-2周）；长期储存-20℃（12个月）；收货后建议分装，避免反复冻融。
分子类型	抗体

关联靶点（人）

DNM1L Tchem 动力蛋白-1类蛋白(Dynamin-1-like protein) (0 活性数据)

点击查看靶蛋白信息： DNM1L

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

应用

应用名称	稀释比例
IF/ICC	1:100
WB	1/4000
IHC	1/150

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

引用文献

1. Han H et al.. (2020) PINK1 phosphorylates Drp1S616 to regulate mitophagy-independent mitochondrial dynamics.. EMBO Rep, 21 (8): (e48686). [PMID:32484300]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
3. Shin, H W HW, Shinotsuka, C C, Torii, S S, Murakami, K K and Nakayama, K K.. (1997) Identification and subcellular localization of a novel mammalian dynamin-related protein homologous to yeast Vps1p and Dnm1p.. Journal of biochemistry, [PMID:9348079]
4. Kamimoto, T T and 5 more authors.. (1998) Dymple, a novel dynamin-like high molecular weight GTPase lacking a proline-rich carboxyl-terminal domain in mammalian cells.. The Journal of biological chemistry, (9): [PMID:9422767]
5. Yoon, Y Y, Pitts, K R KR, Dahan, S S and McNiven, M A MA.. (1998) A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells.. The Journal of cell biology, (23): [PMID:9472031]
6. Imoto, M M, Tachibana, I I and Urrutia, R R.. (1998) Identification and functional characterization of a novel human protein highly related to the yeast dynamin-like GTPase Vps1p.. Journal of cell science, [PMID:9570752]
7. Hong, Y R YR, Chen, C H CH, Cheng, D S DS, Howng, S L SL and Chow, C C CC.. (1998) Human dynamin-like protein interacts with the glycogen synthase kinase 3beta.. Biochemical and biophysical research communications, (28): [PMID:9731200]
8. Smirnova, E E, Shurland, D L DL, Ryazantsev, S N SN and van der Bliek, A M AM.. (1998) A human dynamin-related protein controls the distribution of mitochondria.. The Journal of cell biology, (19): [PMID:9786947]
9. Chen, C H CH and 5 more authors.. (2000) Differential expression of four human dynamin-like protein variants in brain tumors.. DNA and cell biology, [PMID:10749171]
10. Smirnova, E E, Griparic, L L, Shurland, D L DL and van der Bliek, A M AM.. (2001) Dynamin-related protein Drp1 is required for mitochondrial division in mammalian cells.. Molecular biology of the cell, [PMID:11514614]
11. Koch, Annett A and 5 more authors.. (2003) Dynamin-like protein 1 is involved in peroxisomal fission.. The Journal of biological chemistry, (7): [PMID:12499366]
12. Li, Xiaoling X and Gould, Stephen J SJ.. (2003) The dynamin-like GTPase DLP1 is essential for peroxisome division and is recruited to peroxisomes in part by PEX11.. The Journal of biological chemistry, (9): [PMID:12618434]
13. Scherer, Steven E SE and 216 more authors.. (2006) The finished DNA sequence of human chromosome 12.. Nature, (16): [PMID:16541075]
14. Yonashiro, Ryo R and 11 more authors.. (2006) A novel mitochondrial ubiquitin ligase plays a critical role in mitochondrial dynamics.. The EMBO journal, (9): [PMID:16874301]
15. Nakamura, Nobuhiro N, Kimura, Yasuo Y, Tokuda, Masaki M, Honda, Shinji S and Hirose, Shigehisa S.. (2006) MARCH-V is a novel mitofusin 2- and Drp1-binding protein able to change mitochondrial morphology.. EMBO reports, [PMID:16936636]
16. Parone, Philippe A PA and 6 more authors.. (2006) Inhibiting the mitochondrial fission machinery does not prevent Bax/Bak-dependent apoptosis.. Molecular and cellular biology, [PMID:17015472]
17. Taguchi, Naoko N, Ishihara, Naotada N, Jofuku, Akihiro A, Oka, Toshihiko T and Mihara, Katsuyoshi K.. (2007) Mitotic phosphorylation of dynamin-related GTPase Drp1 participates in mitochondrial fission.. The Journal of biological chemistry, (13): [PMID:17301055]
18. Waterham, Hans R HR and 5 more authors.. (2007) A lethal defect of mitochondrial and peroxisomal fission.. The New England journal of medicine, (26): [PMID:17460227]
19. Chang, Chuang-Rung CR and Blackstone, Craig C.. (2007) Cyclic AMP-dependent protein kinase phosphorylation of Drp1 regulates its GTPase activity and mitochondrial morphology.. The Journal of biological chemistry, (27): [PMID:17553808]
20. Karbowski, Mariusz M, Neutzner, Albert A and Youle, Richard J RJ.. (2007) The mitochondrial E3 ubiquitin ligase MARCH5 is required for Drp1 dependent mitochondrial division.. The Journal of cell biology, (2): [PMID:17606867]
21. Zahedi, René P RP and 8 more authors.. (2008) Phosphoproteome of resting human platelets.. Journal of proteome research, [PMID:18088087]
22. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648]
23. Han, Xiao-Jian XJ and 9 more authors.. (2008) CaM kinase I alpha-induced phosphorylation of Drp1 regulates mitochondrial morphology.. The Journal of cell biology, (11): [PMID:18695047]
24. Cereghetti, G M GM and 6 more authors.. (2008) Dephosphorylation by calcineurin regulates translocation of Drp1 to mitochondria.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:18838687]
25. Cho, Dong-Hyung DH and 6 more authors.. (2009) S-nitrosylation of Drp1 mediates beta-amyloid-related mitochondrial fission and neuronal injury.. Science (New York, N.Y.), (3): [PMID:19342591]
26. Braschi, Emélie E, Zunino, Rodolfo R and McBride, Heidi M HM.. (2009) MAPL is a new mitochondrial SUMO E3 ligase that regulates mitochondrial fission.. EMBO reports, [PMID:19407830]
27. Figueroa-Romero, Claudia C and 8 more authors.. (2009) SUMOylation of the mitochondrial fission protein Drp1 occurs at multiple nonconsensus sites within the B domain and is linked to its activity cycle.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, [PMID:19638400]
28. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
29. Bonekamp, Nina A NA, Vormund, Kerstin K, Jacob, Ralf R and Schrader, Michael M.. (2010) Dynamin-like protein 1 at the Golgi complex: a novel component of the sorting/targeting machinery en route to the plasma membrane.. Experimental cell research, (10): [PMID:20688057]
30. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
31. Palmer, Catherine S CS and 5 more authors.. (2011) MiD49 and MiD51, new components of the mitochondrial fission machinery.. EMBO reports, [PMID:21508961]
32. Zhao, Jian J and 9 more authors.. (2011) Human MIEF1 recruits Drp1 to mitochondrial outer membranes and promotes mitochondrial fusion rather than fission.. The EMBO journal, (24): [PMID:21701560]
33. Kashatus, David F DF and 5 more authors.. (2011) RALA and RALBP1 regulate mitochondrial fission at mitosis.. Nature cell biology, (7): [PMID:21822277]
34. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
35. Wang, Zhigao Z, Jiang, Hui H, Chen, She S, Du, Fenghe F and Wang, Xiaodong X.. (2012) The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways.. Cell, (20): [PMID:22265414]
36. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
37. Losón, Oliver C OC, Song, Zhiyin Z, Chen, Hsiuchen H and Chan, David C DC.. (2013) Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in mitochondrial fission.. Molecular biology of the cell, [PMID:23283981]
38. Koirala, Sajjan S and 6 more authors.. (2013) Interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission.. Proceedings of the National Academy of Sciences of the United States of America, (9): [PMID:23530241]
39. Fröhlich, Chris C and 7 more authors.. (2013) Structural insights into oligomerization and mitochondrial remodelling of dynamin 1-like protein.. The EMBO journal, (2): [PMID:23584531]
40. Palmer, Catherine S CS and 5 more authors.. (2013) Adaptor proteins MiD49 and MiD51 can act independently of Mff and Fis1 in Drp1 recruitment and are specific for mitochondrial fission.. The Journal of biological chemistry, (20): [PMID:23921378]
41. Wenger, Julia J and 8 more authors.. (2013) Functional mapping of human dynamin-1-like GTPase domain based on x-ray structure analyses.. PloS one, [PMID:23977156]
42. Shen, Qinfang and 9 more authors.. (2014) Mutations in Fis1 disrupt orderly disposal of defective mitochondria.. Molecular biology of the cell, [PMID:24196833]
43. Shahni, Rojeen R and 14 more authors.. (2015) Signal transducer and activator of transcription 2 deficiency is a novel disorder of mitochondrial fission.. Brain : a journal of neurology, [PMID:26122121]
44. Vanstone, Jason R JR and 17 more authors.. (2016) DNM1L-related mitochondrial fission defect presenting as refractory epilepsy.. European journal of human genetics : EJHG, [PMID:26604000]
45. Sheffer, Ruth R and 7 more authors.. (2016) Postnatal microcephaly and pain insensitivity due to a de novo heterozygous DNM1L mutation causing impaired mitochondrial fission and function.. American journal of medical genetics. Part A, [PMID:26992161]
46. Fahrner, Jill A JA, Liu, Raymond R, Perry, Michael Scott MS, Klein, Jessica J and Chan, David C DC.. (2016) A novel de novo dominant negative mutation in DNM1L impairs mitochondrial fission and presents as childhood epileptic encephalopathy.. American journal of medical genetics. Part A, [PMID:27145208]
47. Zaha, K K and 15 more authors.. (2016) DNM1L-related encephalopathy in infancy with Leigh syndrome-like phenotype and suppression-burst.. Clinical genetics, [PMID:27301544]
48. Nasca, Alessia A and 7 more authors.. (2016) Biallelic Mutations in DNM1L are Associated with a Slowly Progressive Infantile Encephalopathy.. Human mutation, [PMID:27328748]
49. Gerber, Sylvie S and 25 more authors.. (2017) Mutations in DNM1L, as in OPA1, result in dominant optic atrophy despite opposite effects on mitochondrial fusion and fission.. Brain : a journal of neurology, (1): [PMID:28969390]
50. Kalia, Raghav R and 6 more authors.. (2018) Structural basis of mitochondrial receptor binding and constriction by DRP1.. Nature, [PMID:29899447]
51. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

DRP1 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器