CD40 Mouse mAb

COA

级别和纯度:

无载体

已验证

ExactAb™

见COA

应用:

Flow Cytometry

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG2b
克隆号: HI40a
偶联: Unconjugated

有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab094763-10μg	10μg	现货
Ab094763-50μg	50μg	现货
Ab094763-100μg	100μg	期货
Ab094763-1mg	1mg	期货

查看相关系列

点击查看靶蛋白信息: CD40

Accession#:P25942 (34) CD40 (40) Gene ID:958 (33) 流式细胞术 (1163)

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基本信息

产品名称	CD40 Mouse mAb
别名	CD40单克隆抗体 \| Anti-CD40抗体 \| CD40抗体 \| CD40单抗 \| CD40小鼠单克隆抗体 \| CD40小鼠单抗
英文别名	B cell surface antigen CD40 \| B-cell surface antigen CD40 \| Bp50B cell-associated molecule \| CD40 antigen \| CD40 molecule, TNF receptor superfamily member 5 \| CD40 type II isoform \| CD40 \| CD40L receptor \| CDw40 \| MGC9013 \| nerve growth factor receptor-re
规格或纯度	无载体, ExactAb™, 已验证, 见COA
宿主种属	小鼠(Mouse)
特异性	CD40
种属反应性	人(Human)
预测反应种属	狗(Dog),鸡(Chicken)
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	HI40a
Format	Whole IgG
亚型	Mouse IgG2b
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 0.1% Sodium azide, pH 7.2
防腐剂	0.1% Sodium azide
浓度	见COA
储存温度	2-8°C储存
运输条件	冰袋运输
稳定性与储存	4°C 下运输。4°C 长期保存（36 个月）。收货后建议分装。
分子类型	抗体

关联靶点（人）

CD40 Tchem 肿瘤坏死因子受体超家族成员 5(Tumor necrosis factor receptor superfamily member 5) (0 活性数据)

点击查看靶蛋白信息： CD40

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

CD40 Tchem Tumor necrosis factor receptor superfamily member 5 (9 活性数据)

点击查看靶蛋白信息： CD40

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Flow Cytometry: CD40 Mouse mAb (Ab094763)

CD40 Mouse mAb (Ab094763) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD40 (red) with CD40 Mouse mAb (Ab094763) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	< 0.5µg/100w cells

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0102452	分析证书	Ab094763
ZJ24F0102451	分析证书	Ab094763
ZJ24F0102450	分析证书	Ab094763

技术文档和文章

利用甲醇通透法进行流式细胞术

Categories: 实验方案（Protocols）

探索主题:

流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

可替换产品

引用文献

1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052]
2. Chen J, Song Y, Bojadzic D, Tamayo-Garcia A, Landin AM, Blomberg BB, Buchwald P.. (2017) Small-Molecule Inhibitors of the CD40-CD40L Costimulatory Protein-Protein Interaction.. J Med Chem, 60 (21): (8906-8922). [PMID:29024591] [10.1021/acs.jmedchem.7b01154]
3. Braesch-Andersen, S S and 5 more authors.. (1989) Biochemical characteristics and partial amino acid sequence of the receptor-like human B cell and carcinoma antigen CDw40.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2463309]
4. Stamenkovic, I I, Clark, E A EA and Seed, B B.. (1989) A B-lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas.. The EMBO journal, [PMID:2475341]
5. Sato, T T, Irie, S S and Reed, J C JC.. (1995) A novel member of the TRAF family of putative signal transducing proteins binds to the cytosolic domain of CD40.. FEBS letters, (23): [PMID:7530216]
6. Cheng, G G and 5 more authors.. (1995) Involvement of CRAF1, a relative of TRAF, in CD40 signaling.. Science (New York, N.Y.), (10): [PMID:7533327]
7. Bajorath, J J and Aruffo, A A.. (1997) Construction and analysis of a detailed three-dimensional model of the ligand binding domain of the human B cell receptor CD40.. Proteins, [PMID:9037712]
8. Kashiwada, M M and 8 more authors.. (1998) Tumor necrosis factor receptor-associated factor 6 (TRAF6) stimulates extracellular signal-regulated kinase (ERK) activity in CD40 signaling along a ras-independent pathway.. The Journal of experimental medicine, (19): [PMID:9432981]
9. Mizushima, S S and 8 more authors.. (1998) Cloning and characterization of a cDNA encoding the human homolog of tumor necrosis factor receptor-associated factor 5 (TRAF5).. Gene, (30): [PMID:9511754]
10. Singh, J J and 7 more authors.. (1998) The role of polar interactions in the molecular recognition of CD40L with its receptor CD40.. Protein science : a publication of the Protein Society, [PMID:9605317]
11. Pullen, S S SS and 5 more authors.. (1998) CD40-tumor necrosis factor receptor-associated factor (TRAF) interactions: regulation of CD40 signaling through multiple TRAF binding sites and TRAF hetero-oligomerization.. Biochemistry, (25): [PMID:9718306]
12. Ni, C Z CZ and 6 more authors.. (2000) Molecular basis for CD40 signaling mediated by TRAF3.. Proceedings of the National Academy of Sciences of the United States of America, (12): [PMID:10984535]
13. Tone, M M, Tone, Y Y, Fairchild, P J PJ, Wykes, M M and Waldmann, H H.. (2001) Regulation of CD40 function by its isoforms generated through alternative splicing.. Proceedings of the National Academy of Sciences of the United States of America, (13): [PMID:11172023]
14. Ferrari, S S and 15 more authors.. (2001) Mutations of CD40 gene cause an autosomal recessive form of immunodeficiency with hyper IgM.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:11675497]
15. Khandekar, S S SS and 9 more authors.. (2001) Determination of carbohydrate structures N-linked to soluble CD154 and characterization of the interactions of CD40 with CD154 expressed in Pichia pastoris and Chinese hamster ovary cells.. Protein expression and purification, [PMID:11676606]
16. Li, Chenglong C and 9 more authors.. (2002) Downstream regulator TANK binds to the CD40 recognition site on TRAF3.. Structure (London, England : 1993), [PMID:12005438]
17. Tai, Yu-Tzu YT and 13 more authors.. (2004) Mechanisms by which SGN-40, a humanized anti-CD40 antibody, induces cytotoxicity in human multiple myeloma cells: clinical implications.. Cancer research, (15): [PMID:15087402]
18. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161]
19. Ouadani, Hanen H and 14 more authors.. (2016) Novel and recurrent AID mutations underlie prevalent autosomal recessive form of HIGM in consanguineous patients.. Immunogenetics, [PMID:26545377]
20. Takada, Yoko K; Yu, Jessica; Shimoda, Michiko and Takada, Yoshikazu.. (2019) Integrin Binding to the Trimeric Interface of CD40L Plays a Critical Role in CD40/CD40L Signaling.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:31331973]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

CD40 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器