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CD32 Mouse mAb

    应用:
  • Flow Cytometry
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG2b
  • 克隆号: IV.3
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab094575-10μg
10μg 现货 Stock Image
Ab094575-50μg
50μg 现货 Stock Image
Ab094575-100μg
100μg 期货 Stock Image
Ab094575-1mg
1mg 期货 Stock Image

基本信息

产品名称 CD32 Mouse mAb
别名 免疫球蛋白G Fc段受体Ⅱ抗体 | CD32抗体 | CD32小鼠单克隆抗体 | CD32小鼠单抗
英文别名 CD32 antigen | CD32B | CD32Fc fragment of IgG, low affinity II, receptor for (CD32) | CDw32 | Fc fragment of IgG, low affinity IIb, receptor (CD32) | Fc fragment of IgG, low affinity IIb, receptor for (CD32) | Fc gamma RII | Fc gamma RIIb | FCG2 | Fc-gamm
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属 小鼠(Mouse)
特异性 CD32
种属反应性 人(Human)
免疫原 The FcR of K562 cells
阳性对照 Flow: Human peripheral blood lymphocytes.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 IV.3
Format Whole IgG
亚型 Mouse IgG2b
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein G purified
物理外观 Liquid
储存缓冲液 10mM PBS, 0.1% Proclin 300, pH 7.2-7.4
防腐剂 0.1% Proclin 300
浓度 见COA
储存温度 2-8°C储存
运输条件 冰袋运输
稳定性与储存 4°C 下运输。4°C 长期保存(12 个月)。收货后建议分装。
分子类型 抗体

关联靶点(人)

FCGR2A Tbio 低亲和力免疫球蛋白γFc区受体II-a(Low affinity immunoglobulin gamma Fc region receptor II-a) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

CD32 Mouse mAb (Ab094575) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD32 with CD32 Mouse mAb (Ab094575) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

CD32 Mouse mAb (Ab094575) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD32 (red) with CD32 Mouse mAb (Ab094575) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

CD32 Mouse mAb (Ab094575) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling CD32 (red) with CD32 Mouse mAb (Ab094575) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称 稀释比例
Flow Cytometry

1μg/100w cells

质检证书(CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ24F1011864 分析证书 Ab094575
ZJ23F1202100 分析证书 Ab094575
ZJ23F1202101 分析证书 Ab094575

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引用文献

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2. Brooks, D G DG, Qiu, W Q WQ, Luster, A D AD and Ravetch, J V JV..  (1989)  Structure and expression of human IgG FcRII(CD32). Functional heterogeneity is encoded by the alternatively spliced products of multiple genes..  The Journal of experimental medicine,  (1): [PMID:2529342]
3. Stuart, S G SG and 6 more authors..  (1987)  Isolation and expression of cDNA clones encoding a human receptor for IgG (Fc gamma RII)..  The Journal of experimental medicine,  (1): [PMID:2824655]
4. Hibbs, M L ML, Bonadonna, L L, Scott, B M BM, McKenzie, I F IF and Hogarth, P M PM..  (1988)  Molecular cloning of a human immunoglobulin G Fc receptor..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:2965389]
5. Stengelin, S S, Stamenkovic, I I and Seed, B B..  (1988)  Isolation of cDNAs for two distinct human Fc receptors by ligand affinity cloning..  The EMBO journal,  [PMID:3402431]
6. Hamada, F F, Aoki, M M, Akiyama, T T and Toyoshima, K K..  (1993)  Association of immunoglobulin G Fc receptor II with Src-like protein-tyrosine kinase Fgr in neutrophils..  Proceedings of the National Academy of Sciences of the United States of America,  (1): [PMID:8327512]
7. Salmon, J E JE and 8 more authors..  (1996)  Fc gamma RIIA alleles are heritable risk factors for lupus nephritis in African Americans..  The Journal of clinical investigation,  (1): [PMID:8636449]
8. Bewarder, N N and 6 more authors..  (1996)  In vivo and in vitro specificity of protein tyrosine kinases for immunoglobulin G receptor (FcgammaRII) phosphorylation..  Molecular and cellular biology,  [PMID:8756631]
9. Maxwell, K F KF and 6 more authors..  (1999)  Crystal structure of the human leukocyte Fc receptor, Fc gammaRIIa..  Nature structural biology,  [PMID:10331870]
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12. Lin, Thomas S TS and 9 more authors..  (2005)  FCGR3A and FCGR2A polymorphisms may not correlate with response to alemtuzumab in chronic lymphocytic leukemia..  Blood,  (1): [PMID:15217834]
13. Criswell, Lindsey A LA and 11 more authors..  (2004)  The influence of genetic variation in the HLA-DRB1 and LTA-TNF regions on the response to treatment of early rheumatoid arthritis with methotrexate or etanercept..  Arthritis and rheumatism,  [PMID:15457442]
14. Hughes, L B LB and 7 more authors..  (2004)  Genetic risk factors for infection in patients with early rheumatoid arthritis..  Genes and immunity,  [PMID:15526004]
15. Wang, Yaning Y, Fei, David D, Vanderlaan, Martin M and Song, An A..  (2004)  Biological activity of bevacizumab, a humanized anti-VEGF antibody in vitro..  Angiogenesis,  [PMID:15886877]
16. Gregory, S G SG and 178 more authors..  (2006)  The DNA sequence and biological annotation of human chromosome 1..  Nature,  (18): [PMID:16710414]
17. Zhang, Wu W and 12 more authors..  (2007)  FCGR2A and FCGR3A polymorphisms associated with clinical outcome of epidermal growth factor receptor expressing metastatic colorectal cancer patients treated with single-agent cetuximab..  Journal of clinical oncology : official journal of the American Society of Clinical Oncology,  (20): [PMID:17704420]
18. Lu, Jinghua J and 5 more authors..  (2008)  Structural recognition and functional activation of FcgammaR by innate pentraxins..  Nature,  (18): [PMID:19011614]
19. Seimetz, Diane D, Lindhofer, Horst H and Bokemeyer, Carsten C..  (2010)  Development and approval of the trifunctional antibody catumaxomab (anti-EpCAM x anti-CD3) as a targeted cancer immunotherapy..  Cancer treatment reviews,  [PMID:20347527]

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