Caspase-9 Mouse mAb

COA

级别和纯度:

已验证

ExactAb™

2.05 mg/mL

应用:

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 小鼠(Mouse)
亚型: Mouse IgG1
克隆号: 1D1-F2-E9
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab093085-10μl	10μl	现货
Ab093085-50μl	50μL	现货
Ab093085-100μl	100μL	现货
Ab093085-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: CASP9

Accession#:P55211 (4) CASP9 (4) Caspase-9 (3) Gene ID:842 (4)

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基本信息

产品名称	Caspase-9 Mouse mAb
别名	Caspase-9单抗 \| Caspase-9抗体 \| 活化半胱胺酸蛋白酶蛋白-9抗体 \| Caspase-9 小鼠 mAb
英文别名	CASP9 \| MCH6 \| Caspase-9 \| CASP-9 \| Apoptotic protease Mch-6 \| Apoptotic protease-activating factor 3 \| APAF-3 \| ICE-like apoptotic protease 6 \| ICE-LAP6 \| APAF-3 antibody \| APAF3 antibody \| Apoptosis related cysteine peptidase antibody \| Apoptotic protea
规格或纯度	ExactAb™, Validated, 2.05 mg/mL
宿主种属	小鼠(Mouse)
特异性	CASP9
种属反应性	人(Human),小鼠(Mouse)
免疫原	Purified recombinant human Caspase-9 protein fragments expressed in E.coli (AA 1-416).
阳性对照	WB: A549, A431, Jurkat, K562, NIH/3T3 and HeLa cell lysates.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	1D1-F2-E9
Format	Whole IgG
亚型	Mouse IgG1
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.5% BSA, 0.02% Sodium azide, pH 7.3
防腐剂	0.02% Sodium azide
浓度	2.05 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

CASP9 Tchem 胱天蛋白酶-9(Caspase-9) (0 活性数据)

点击查看靶蛋白信息： CASP9

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

CASP9 Tchem Caspase-9 (154 活性数据)

点击查看靶蛋白信息： CASP9

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Caspase-9 Mouse mAb (Ab093085)

Caspase-9 Mouse mAb (Ab093085) - Western Blot
All lanes: Caspase-9 Mouse mAb (Ab093085) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution

Predicted band size: 46 kDa
Observed band size: 27, 43, 45 kDa
Exposure time: 30 s

应用

应用名称	稀释比例
WB	1/500-1/1000

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到4个结果

批号(Lot Number)	证书类型	货号
ZJ23F0901049	分析证书	Ab093085
ZJ23F0901048	分析证书	Ab093085
ZJ25F0319504	分析证书	Ab093085
ZJ24F0404300	分析证书	Ab093085

技术文档和文章

蛋白质印迹的一抗选择指南

Categories: 实验方案（Protocols）

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重组抗体单克隆抗体多克隆抗体蛋白质印迹内参抗体一抗选择

Guidelines of Primary Antibodies for Western Blotting

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Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

Guidelines of Primary Antibodies for Western Blotting

Categories: 实验方案（Protocols）

探索主题:

Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

Caspase免疫荧光染色方案

Categories: 实验方案（Protocols）

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二抗细胞凋亡程序性死亡免疫荧光半胱天冬酶

Caspase Immunofluorescence Staining Protocol

Categories: 实验方案（Protocols）

探索主题:

Secondary antibody Apoptosis Programmed Death Immunofluorescence Caspase

Caspase Immunofluorescence Staining Protocol

Categories: 实验方案（Protocols）

探索主题:

Secondary antibody Apoptosis Programmed Death Immunofluorescence Caspase

高分子量蛋白质印迹法

Categories: 实验方案（Protocols）

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免疫印迹生物缓冲液蛋白质印迹高分子量蛋白大分子蛋白蛋白质电泳 WB抗体

Protocol of Western Blotting For High Molecular Weights

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

细胞凋亡诱导方法

Categories: 实验方案（Protocols）

探索主题:

细胞凋亡程序性死亡 Caspase 凋亡诱导化学诱导生物诱导凋亡因子 Fas CD95

引用文献

1. Mocanu MM, Baxter GF, Yellon DM. (2000) Caspase inhibition and limitation of myocardial infarct size: protection against lethal reperfusion injury.. Br J Pharmacol, 130 (2): (197-200). [PMID:10807653]
2. Zhou G, Zorn N, Ting P, Aslanian R, Lin M, Cook J, Lachowicz J, Lin A, Smith M, Hwa J et al.. (2014) Development of novel benzomorpholine class of diacylglycerol acyltransferase I inhibitors.. ACS Med Chem Lett, 5 (5): (544-9). [PMID:24900877]
3. Kwan JC, Eksioglu EA, Liu C, Paul VJ, Luesch H. (2009) Grassystatins A-C from marine cyanobacteria, potent cathepsin E inhibitors that reduce antigen presentation.. J Med Chem, 52 (18): (5732-47). [PMID:19715320]
4. Salvador LA, Taori K, Biggs JS, Jakoncic J, Ostrov DA, Paul VJ, Luesch H.. (2013) Potent elastase inhibitors from cyanobacteria: structural basis and mechanisms mediating cytoprotective and anti-inflammatory effects in bronchial epithelial cells.. J Med Chem, 56 (3): (1276-1290). [PMID:23350733] [10.1021/jm3017305]
5. Duan, H H and 6 more authors.. (1996) ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B.. The Journal of biological chemistry, (12): [PMID:8663294]
6. Srinivasula, S M SM and 9 more authors.. (1996) The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32.. The Journal of biological chemistry, (25): [PMID:8900201]
7. Seol, D W DW and Billiar, T R TR.. (1999) A caspase-9 variant missing the catalytic site is an endogenous inhibitor of apoptosis.. The Journal of biological chemistry, (22): [PMID:9890966]
8. Srinivasula, S M SM and 6 more authors.. (1999) Identification of an endogenous dominant-negative short isoform of caspase-9 that can regulate apoptosis.. Cancer research, (1): [PMID:10070954]
9. Hadano, S S and 9 more authors.. (1999) Genomic organization of the human caspase-9 gene on Chromosome 1p36. 1-p36.3.. Mammalian genome : official journal of the International Mammalian Genome Society, [PMID:10384055]
10. Renatus, M M, Stennicke, H R HR, Scott, F L FL, Liddington, R C RC and Salvesen, G S GS.. (2001) Dimer formation drives the activation of the cell death protease caspase 9.. Proceedings of the National Academy of Sciences of the United States of America, (4): [PMID:11734640]
11. Riebeling, C C, Forsea, A-M AM, Raisova, M M, Orfanos, C E CE and Geilen, C C CC.. (2002) The bisphosphonate pamidronate induces apoptosis in human melanoma cells in vitro.. British journal of cancer, (29): [PMID:12177810]
12. Shiozaki, Eric N EN and 8 more authors.. (2003) Mechanism of XIAP-mediated inhibition of caspase-9.. Molecular cell, [PMID:12620238]
13. Allan, Lindsey A LA and 5 more authors.. (2003) Inhibition of caspase-9 through phosphorylation at Thr 125 by ERK MAPK.. Nature cell biology, [PMID:12792650]
14. Bartke, Till T, Pohl, Christian C, Pyrowolakis, George G and Jentsch, Stefan S.. (2004) Dual role of BRUCE as an antiapoptotic IAP and a chimeric E2/E3 ubiquitin ligase.. Molecular cell, (18): [PMID:15200957]
15. Raina, Deepak D and 7 more authors.. (2005) c-Abl tyrosine kinase regulates caspase-9 autocleavage in the apoptotic response to DNA damage.. The Journal of biological chemistry, (25): [PMID:15657060]
16. Wada, Akira A and 7 more authors.. (2006) Pamidronate induced anti-proliferative, apoptotic, and anti-migratory effects in hepatocellular carcinoma.. Journal of hepatology, [PMID:16298452]
17. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414]
18. Wang, Pingzhang P, Shi, Taiping T and Ma, Dalong D.. (2006) Cloning of a novel human caspase-9 splice variant containing only the CARD domain.. Life sciences, (1): [PMID:16780893]
19. Han, Yuchi Y and 7 more authors.. (2006) Overexpression of HAX-1 protects cardiac myocytes from apoptosis through caspase-9 inhibition.. Circulation research, (18): [PMID:16857965]
20. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648]
21. Kim, Jae-Hong JH and 8 more authors.. (2009) HIP1R interacts with a member of Bcl-2 family, BCL2L10, and induces BAK-dependent cell death.. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, [PMID:19255499]
22. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
23. Blasche, Sonja and 12 more authors.. (2013) The E. coli effector protein NleF is a caspase inhibitor.. PloS one, [PMID:23516580]
24. Eron, Scott J SJ, Raghupathi, Kishore K and Hardy, Jeanne A JA.. (2017) Dual Site Phosphorylation of Caspase-7 by PAK2 Blocks Apoptotic Activity by Two Distinct Mechanisms.. Structure (London, England : 1993), (3): [PMID:27889207]
25. Smith, Christina E and 5 more authors.. (2017) Non-steroidal Anti-inflammatory Drugs Are Caspase Inhibitors.. Cell chemical biology, (16): [PMID:28238723]

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稀释计算器

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复溶计算器

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体积（添加到小瓶中）

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Caspase-9 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器