Recombinant Bax Antibody

COA

级别和纯度:

0.25mg/mL

应用:

Flow Cytometry
IF/ICC
IHC
IP
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Rabbit IgG
克隆号: RR647
偶联: Unconjugated

有货

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库存信息

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货号 (SKU)	包装规格	是否现货	价格	数量
Ab090652-50μl	50μL	现货
Ab090652-100μl	100μL	现货

查看相关系列

点击查看靶蛋白信息: BAX

Accession#:Q07812 (5) BAX (5) Gene ID:581 (5) 流式细胞术 (1163)

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基本信息

产品名称	Recombinant Bax Antibody
别名	Bax抗体 \| anti- Bax抗体 \| Bcl2关联X蛋白抗体 \| 重组Bax抗体
英文别名	Apoptosis regulator BAX antibody \| BAX antibody \| Bax-protein antibody \| BAX_HUMAN antibody \| BAXA antibody \| Baxdelta2G9 antibody \| Baxdelta2G9omega antibody \| Baxdelta2omega antibody \| Bcl-2-like protein 4 antibody \| BCL2 associated X protein antibody \|
规格或纯度	ExactAb™, Validated, Recombinant, High performance, 0.25mg/mL
宿主种属	兔(Rabbit)
特异性	Bax
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	A synthetic peptide of human BAX (AA 1-100).
阳性对照	WB: A549, HT-29, HeLa, LNCaP, Hep G2 and HKE293 cell lysates. IHC: Human Ovarian cancer tissue. IF/ICC: HeLa cells. Flow: HeLa cells. IP: HT-29 whole cell lysate.
偶联	Unconjugated
备注	本产品即将售完下架，替代产品为 Ab090655

产品属性

克隆类型	重组抗体
克隆号	RR647
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3.
防腐剂	0.01% Sodium azide
浓度	0.25mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

BAX Tchem 细胞凋亡调节剂BAX(Apoptosis regulator BAX) (0 活性数据)

点击查看靶蛋白信息： BAX

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

BAX Tchem Apoptosis regulator BAX (73 活性数据)

点击查看靶蛋白信息： BAX

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Recombinant Bax Antibody (Ab090652)

Recombinant Bax Antibody (Ab090652) - Western Blot
All lanes: Recombinant Bax Antibody (Ab090652) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 21 kDa
Observed band size: 20 kDa
Exposure time: 6.2 s

IHC: Recombinant Bax Antibody (Ab090652)

Recombinant Bax Antibody (Ab090652) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue labelling Bax with Recombinant Bax Antibody (Ab090652) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Flow Cytometry: Recombinant Bax Antibody (Ab090652)

Recombinant Bax Antibody (Ab090652) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant Bax Antibody (Ab090652) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant Bax Antibody (Ab090652) (1/400 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.

IP: Recombinant Bax Antibody (Ab090652)

Recombinant Bax Antibody (Ab090652) - IP
Bax was immunoprecipitated from 0.5 mg HT-29 whole cell lysate with Recombinant Bax Antibody (Ab090652) at 1/25 dilution.
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant Bax Antibody (Ab090652) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant Bax Antibody (Ab090652) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)

Exposure: 30 s.

IF/ICC: Recombinant Bax Antibody (Ab090652)

Recombinant Bax Antibody (Ab090652) - IF/ICC
IF analysis of Recombinant Bax Antibody (Ab090652) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Bax Antibody (Ab090652) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab170148) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

应用

应用名称	稀释比例
IP	1/25
IHC	1/1600-1/3200
WB	1/1000-1/2500
Flow Cytometry	1/100-1/400
IF/ICC	1:1000

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找到5个结果

批号(Lot Number)	证书类型	货号
ZJ23F0901012	分析证书	Ab090652
ZJ23F0901011	分析证书	Ab090652
ZJ25F0319505	分析证书	Ab090652
ZJ24F0911347	分析证书	Ab090652
ZJ24F0404255	分析证书	Ab090652

技术文档和文章

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Categories: 技术文章

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流式细胞术

流式细胞仪分析

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重组抗体单克隆抗体 IHC 免疫组化冷冻切片

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General Immunoprecipitation Protocol

Categories: 实验方案（Protocols）

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Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads

General Immunoprecipitation Protocol

Categories: 实验方案（Protocols）

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Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads

General Immunoprecipitation Protocol

Categories: 实验方案（Protocols）

探索主题:

Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads

General Immunoprecipitation Protocol

Categories: 实验方案（Protocols）

探索主题:

Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads

RNA免疫沉淀方案

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RNA Immunoprecipitation (RIP) Protocol

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IP Protein A/G magnetic beads RIP Input RNA immunoprecipitation RNA protein interaction Isotype control antibody Crosslinking

RNA Immunoprecipitation (RIP) Protocol

Categories: 实验方案（Protocols）

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IP Protein A/G magnetic beads RIP Input RNA immunoprecipitation RNA protein interaction Isotype control antibody Crosslinking

固定免疫沉淀实验方案

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Immobilized IP Protocol

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Immunoprecipitation WB Western blot IP Protein interaction Immobilization immunoprecipitation Immobilized antibody

Immobilized IP Protocol

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Immunoprecipitation WB Western blot IP Protein interaction Immobilization immunoprecipitation Immobilized antibody

细胞凋亡诱导方法

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Flow Cytometry Using Methanol Permeabilization

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探索主题:

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引用文献

1. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
3. Feng R, Ni HM, Wang SY, Tourkova IL, Shurin MR, Harada H, Yin XM.. (2007) Cyanidin-3-rutinoside, a natural polyphenol antioxidant, selectively kills leukemic cells by induction of oxidative stress.. J Biol Chem, 282 (18): (13468-13476). [PMID:17360708] [10.1074/jbc.m610616200]
4. Zhang Z, Wu G, Xie F, Song T, Chang X.. (2011) 3-Thiomorpholin-8-oxo-8H-acenaphtho[1,2-b]pyrrole-9-carbonitrile (S1) based molecules as potent, dual inhibitors of B-cell lymphoma 2 (Bcl-2) and myeloid cell leukemia sequence 1 (Mcl-1): structure-based design and structure-activity relationship studies.. J Med Chem, 54 (4): (1101-1105). [PMID:21235240] [10.1021/jm101181u]
5. Liu G, Yin T, Kim H, Ding C, Yu Z, Wang H, Chen H, Yan R, Wold EA, Zou H, Liu X, Ding Y, Shen Q, Zhou J.. (2019) Structure-activity relationship studies on Bax activator SMBA1 for the treatment of ER-positive and triple-negative breast cancer.. Eur J Med Chem, 178 (589-605). [PMID:31220676] [10.1016/j.ejmech.2019.06.004]
6. Meijerink, J P JP, Smetsers, T F TF, Slöetjes, A W AW, Linders, E H EH and Mensink, E J EJ.. (1995) Bax mutations in cell lines derived from hematological malignancies.. Leukemia, [PMID:7475270]
7. Apte, S S SS, Mattei, M G MG and Olsen, B R BR.. (1995) Mapping of the human BAX gene to chromosome 19q13.3-q13.4 and isolation of a novel alternatively spliced transcript, BAX delta.. Genomics, (10): [PMID:7607685]
8. Oltvai, Z N ZN, Milliman, C L CL and Korsmeyer, S J SJ.. (1993) Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death.. Cell, (27): [PMID:8358790]
9. Chittenden, T T and 7 more authors.. (1995) A conserved domain in Bak, distinct from BH1 and BH2, mediates cell death and protein binding functions.. The EMBO journal, (15): [PMID:8521816]
10. Meijerink, J P JP and 7 more authors.. (1998) Hematopoietic malignancies demonstrate loss-of-function mutations of BAX.. Blood, (15): [PMID:9531611]
11. Shi, B B and 5 more authors.. (1999) Identification and characterization of baxepsilon, a novel bax variant missing the BH2 and the transmembrane domains.. Biochemical and biophysical research communications, (27): [PMID:9920818]
12. Schmitt, E E, Paquet, C C, Beauchemin, M M, Dever-Bertrand, J J and Bertrand, R R.. (2000) Characterization of Bax-sigma, a cell death-inducing isoform of Bax.. Biochemical and biophysical research communications, (21): [PMID:10772918]
13. Tan, K O KO and 6 more authors.. (2001) MAP-1, a novel proapoptotic protein containing a BH3-like motif that associates with Bax through its Bcl-2 homology domains.. The Journal of biological chemistry, (26): [PMID:11060313]
14. Suzuki, M M, Youle, R J RJ and Tjandra, N N.. (2000) Structure of Bax: coregulation of dimer formation and intracellular localization.. Cell, (10): [PMID:11106734]
15. Cuddeback, S M SM and 7 more authors.. (2001) Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax.. The Journal of biological chemistry, (8): [PMID:11259440]
16. Cartron, Pierre-François PF and 7 more authors.. (2002) The expression of a new variant of the pro-apoptotic molecule Bax, Baxpsi, is correlated with an increased survival of glioblastoma multiforme patients.. Human molecular genetics, (15): [PMID:11912183]
17. Guo, Bin B and 6 more authors.. (2003) Humanin peptide suppresses apoptosis by interfering with Bax activation.. Nature, (22): [PMID:12732850]
18. Gustafsson, Asa B AB, Tsai, Joseph G JG, Logue, Susan E SE, Crow, Michael T MT and Gottlieb, Roberta A RA.. (2004) Apoptosis repressor with caspase recruitment domain protects against cell death by interfering with Bax activation.. The Journal of biological chemistry, (14): [PMID:15004034]
19. Zhang, Honglai H and 5 more authors.. (2005) Clusterin inhibits apoptosis by interacting with activated Bax.. Nature cell biology, [PMID:16113678]
20. Tan, Kuan Onn KO and 7 more authors.. (2005) MAP-1 is a mitochondrial effector of Bax.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:16199525]
21. Nitta, Takeshi T and 8 more authors.. (2006) IAN family critically regulates survival and development of T lymphocytes.. PLoS biology, [PMID:16509771]
22. Peyerl, F W FW and 6 more authors.. (2007) Elucidation of some Bax conformational changes through crystallization of an antibody-peptide complex.. Cell death and differentiation, [PMID:16946732]
23. Reimers, Kerstin K, Choi, Claudia Y-U CY, Mau-Thek, Eddy E and Vogt, Peter M PM.. (2006) Sequence analysis shows that Lifeguard belongs to a new evolutionarily conserved cytoprotective family.. International journal of molecular medicine, [PMID:16964429]
24. Gavathiotis, Evripidis E and 10 more authors.. (2008) BAX activation is initiated at a novel interaction site.. Nature, (23): [PMID:18948948]
25. Benard, Giovanni G and 6 more authors.. (2010) IBRDC2, an IBR-type E3 ubiquitin ligase, is a regulatory factor for Bax and apoptosis activation.. The EMBO journal, (21): [PMID:20300062]
26. Czabotar, Peter E PE and 5 more authors.. (2011) Mutation to Bax beyond the BH3 domain disrupts interactions with pro-survival proteins and promotes apoptosis.. The Journal of biological chemistry, (4): [PMID:21199865]
27. Edlich, Frank F and 8 more authors.. (2011) Bcl-x(L) retrotranslocates Bax from the mitochondria into the cytosol.. Cell, (1): [PMID:21458670]
28. Zhang, Xiaoping X and 9 more authors.. (2012) Human Bop is a novel BH3-only member of the Bcl-2 protein family.. Protein & cell, [PMID:23055042]
29. Rautureau, G J P GJ and 5 more authors.. (2012) The restricted binding repertoire of Bcl-B leaves Bim as the universal BH3-only prosurvival Bcl-2 protein antagonist.. Cell death & disease, (13): [PMID:23235460]
30. Wei, Bo B and 8 more authors.. (2015) Tom70 mediates Sendai virus-induced apoptosis on mitochondria.. Journal of virology, [PMID:25609812]
31. Kuriyama, Sei S, Tsuji, Tadahiro T, Sakuma, Tetsushi T, Yamamoto, Takashi T and Tanaka, Masamistu M.. (2018) PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer.. Cell death discovery, [PMID:29531808]
32. Morris, Daniel L and 7 more authors.. (2019) Humanin induces conformational changes in the apoptosis regulator BAX and sequesters it into fibers, preventing mitochondrial outer-membrane permeabilization.. The Journal of biological chemistry, (13): [PMID:31690630]
33. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

溶液计算器

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稀释计算器

计算配制储备溶液所需的稀释度。

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复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Recombinant Bax Antibody

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器