NOTCH1 Antibody

COA

级别和纯度:

已验证

ExactAb™

1.5 mg/mL

应用:

IF/ICC
IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Rabbit IgG
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab087287-10μl	10μl	现货
Ab087287-50μl	50μL	现货
Ab087287-100μl	100μL	现货
Ab087287-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: NOTCH1

Accession#:P46531 (3) Gene ID:4851 (3) NOTCH1 (3)

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基本信息

产品名称	NOTCH1 Antibody
别名	Notch1蛋白抗体 \| NOTCH1 抗体
英文别名	hN1 antibody \| Neurogenic locus Notch homolog protein 1 antibody \| NICD antibody \| NOTC1_HUMAN antibody \| Notch 1 antibody \| Notch 1 intracellular domain antibody \| Notch homolog 1 translocation associated (Drosophila) antibody \| notch1 antibody \| TAN1 an
规格或纯度	ExactAb™, Validated, 1.5 mg/mL
宿主种属	兔(Rabbit)
特异性	NOTCH1
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	A synthetic peptide derived from human Notch1 (AA 2505-2519).
阳性对照	WB: MCF-7, Jurkat, HEK293 cell lysates; IHC: Human liver; IF: HeLa.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4
防腐剂	0.02% Sodium azide
浓度	1.5 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

NOTCH1 Tchem 神经源性位点 Notch 同源蛋白 1(Neurogenic locus notch homolog protein 1) (0 活性数据)

点击查看靶蛋白信息： NOTCH1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

NOTCH1 Tchem Neurogenic locus notch homolog protein 1 (161 活性数据)

点击查看靶蛋白信息： NOTCH1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: NOTCH1 Antibody (Ab087287)

NOTCH1 Antibody (Ab087287) - Western Blot
All lanes: NOTCH1 Antibody (Ab087287) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 120 kDa
Observed band size: 112 kDa
Exposure time: 3.0 s

IHC: NOTCH1 Antibody (Ab087287)

NOTCH1 Antibody (Ab087287) - IHC
Immunohistochemical analysis of paraffin-embedded Human liver using NOTCH1 Antibody (Ab087287). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

ICC/IF: NOTCH1 Antibody (Ab087287)

NOTCH1 Antibody (Ab087287) - ICC/IF
Immunofluorescent analysis of NOTCH1 in HeLa cells using NOTCH1 Antibody (Ab087287).

应用

应用名称	稀释比例
WB	1/500-1/1000
IHC	1/50 - 1/100
IF/ICC	1/50 - 1/200

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C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F0800794	分析证书	Ab087287
ZJ23F0800793	分析证书	Ab087287
ZJ24F0911312	分析证书	Ab087287

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引用文献

1. Nam Y, Sliz P, Song L, Aster JC, Blacklow SC. (2006) Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes.. Cell, 124 (5): (973-83). [PMID:16530044]
2. Garg V, Muth AN, Ransom JF, Schluterman MK, Barnes R, King IN, Grossfeld PD, Srivastava D. (2005) Mutations in NOTCH1 cause aortic valve disease.. Nature, 437 (7056): (270-4). [PMID:16025100]
3. Stittrich AB, Lehman A, Bodian DL, Ashworth J, Zong Z, Li H, Lam P, Khromykh A, Iyer RK, Vockley JG et al.. (2014) Mutations in NOTCH1 cause Adams-Oliver syndrome.. Am J Hum Genet, 95 (3): (275-84). [PMID:25132448]
4. Astudillo L, Da Silva TG, Wang Z, Han X, Jin K, VanWye J, Zhu X, Weaver K, Oashi T, Lopes PE et al.. (2016) The Small Molecule IMR-1 Inhibits the Notch Transcriptional Activation Complex to Suppress Tumorigenesis.. Cancer Res, 76 (12): (3593-603). [PMID:27197169]
5. Flesch D, Ness J, Lamers C, Dehm F, Popella S, Steri R, Ogorek I, Hieke M, Dannhardt G, Werz O, Weggen S, Schubert-Zsilavecz M.. (2015) SAR-studies of γ-secretase modulators with PPARγ-agonistic and 5-lipoxygenase-inhibitory activity for Alzheimer's disease.. Bioorg Med Chem Lett, 25 (4): (841-846). [PMID:25575659] [10.1016/j.bmcl.2014.12.073]
6. Shan W, Balog A, Quesnelle C, Gill P, Han WC, Norris D, Mandal S, Thiruvenkadam R, Gona KB, Thiyagarajan K, Kandula S, McGlinchey K, Menard K, Wen ML, Rose A, White R, Guarino V, Shen DR, Cvijic ME, Ranasinghe A, Dai J, Zhang Y, Wu DR, Mathur A, Rampulla R, Trainor G, Hunt JT, Vite GD, Westhouse R, Lee FY, Gavai AV.. (2015) BMS-871: a novel orally active pan-Notch inhibitor as an anticancer agent.. Bioorg Med Chem Lett, 25 (9): (1905-1909). [PMID:25857941] [10.1016/j.bmcl.2015.03.038]
7. Yoneyama T, Arai MA, Akamine R, Koryudzu K, Tsuchiya A, Sadhu SK, Ahmed F, Itoh M, Okamoto R, Ishibashi M.. (2017) Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells.. J Nat Prod, 80 (9): (2453-2461). [PMID:28817274] [10.1021/acs.jnatprod.7b00282]
8. Gómez-Galeno JE, Hurtado C, Cheng J, Yardimci C, Mercola M, Cashman JR.. (2018) b-Annulated 1,4-dihydropyridines as Notch inhibitors.. Bioorg Med Chem Lett, 28 (20): (3363-3367). [PMID:30201292] [10.1016/j.bmcl.2018.09.002]
9. Quaglio D, Zhdanovskaya N, Tobajas G, Cuartas V, Balducci S, Christodoulou MS, Fabrizi G, Gargantilla M, Priego EM, Carmona Pestaña Á, Passarella D, Screpanti I, Botta B, Palermo R, Mori M, Ghirga F, Pérez-Pérez MJ.. (2019) Chalcones and Chalcone-mimetic Derivatives as Notch Inhibitors in a Model of T-cell Acute Lymphoblastic Leukemia.. ACS Med Chem Lett, 10 (4): (639-643). [PMID:30996810] [10.1021/acsmedchemlett.8b00608]
10. Ellisen, L W LW and 6 more authors.. (1991) TAN-1, the human homolog of the Drosophila notch gene, is broken by chromosomal translocations in T lymphoblastic neoplasms.. Cell, (23): [PMID:1831692]
11. Matsuno, K K and 7 more authors.. (1998) Human deltex is a conserved regulator of Notch signalling.. Nature genetics, [PMID:9590294]
12. Gray, G E GE and 9 more authors.. (1999) Human ligands of the Notch receptor.. The American journal of pathology, [PMID:10079256]
13. Zhou, S S and 6 more authors.. (2000) SKIP, a CBF1-associated protein, interacts with the ankyrin repeat domain of NotchIC To facilitate NotchIC function.. Molecular and cellular biology, [PMID:10713164]
14. Wu, L L and 5 more authors.. (2000) MAML1, a human homologue of Drosophila mastermind, is a transcriptional co-activator for NOTCH receptors.. Nature genetics, [PMID:11101851]
15. Sakamoto, Kei K and 8 more authors.. (2002) The nephroblastoma overexpressed gene (NOV/ccn3) protein associates with Notch1 extracellular domain and inhibits myoblast differentiation via Notch signaling pathway.. The Journal of biological chemistry, (16): [PMID:12050162]
16. Wu, Lizi L, Sun, Tao T, Kobayashi, Karla K, Gao, Ping P and Griffin, James D JD.. (2002) Identification of a family of mastermind-like transcriptional coactivators for mammalian notch receptors.. Molecular and cellular biology, [PMID:12370315]
17. Vardar, Didem D, North, Christopher L CL, Sanchez-Irizarry, Cheryll C, Aster, Jon C JC and Blacklow, Stephen C SC.. (2003) Nuclear magnetic resonance structure of a prototype Lin12-Notch repeat module from human Notch1.. Biochemistry, (17): [PMID:12795601]
18. Humphray, S J SJ and 147 more authors.. (2004) DNA sequence and analysis of human chromosome 9.. Nature, (27): [PMID:15164053]
19. Hambleton, Sophie S and 7 more authors.. (2004) Structural and functional properties of the human notch-1 ligand binding region.. Structure (London, England : 1993), [PMID:15576031]
20. Ehebauer, Matthias T MT, Chirgadze, Dimitri Y DY, Hayward, Penny P, Martinez Arias, Alfonso A and Blundell, Tom L TL.. (2005) High-resolution crystal structure of the human Notch 1 ankyrin domain.. The Biochemical journal, (15): [PMID:16011479]
21. Coleman, Mathew L ML and 12 more authors.. (2007) Asparaginyl hydroxylation of the Notch ankyrin repeat domain by factor inhibiting hypoxia-inducible factor.. The Journal of biological chemistry, (17): [PMID:17573339]
22. Chastagner, Patricia P, Israël, Alain A and Brou, Christel C.. (2008) AIP4/Itch regulates Notch receptor degradation in the absence of ligand.. PloS one, (16): [PMID:18628966]
23. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648]
24. Brütsch, René R and 8 more authors.. (2010) Integrin cytoplasmic domain-associated protein-1 attenuates sprouting angiogenesis.. Circulation research, (3): [PMID:20616313]
25. Mo, Jung-Soon JS and 13 more authors.. (2011) Serum- and glucocorticoid-inducible kinase 1 (SGK1) controls Notch1 signaling by downregulation of protein stability through Fbw7 ubiquitin ligase.. Journal of cell science, (1): [PMID:21147854]
26. Vasquez-Del Carpio, Rodrigo R and 6 more authors.. (2011) Assembly of a Notch transcriptional activation complex requires multimerization.. Molecular and cellular biology, [PMID:21245387]
27. Gupta-Rossi, Neetu N and 6 more authors.. (2011) The adaptor-associated kinase 1, AAK1, is a positive regulator of the Notch pathway.. The Journal of biological chemistry, (27): [PMID:21464124]
28. Lim, Seung-Oe SO and 7 more authors.. (2011) Notch1 binds and induces degradation of Snail in hepatocellular carcinoma.. BMC biology, (30): [PMID:22128911]
29. Puca, Loredana L, Chastagner, Patricia P, Meas-Yedid, Vannary V, Israël, Alain A and Brou, Christel C.. (2013) Α-arrestin 1 (ARRDC1) and β-arrestins cooperate to mediate Notch degradation in mammals.. Journal of cell science, (1): [PMID:23886940]
30. Boskovski, Marko T MT and 7 more authors.. (2013) The heterotaxy gene GALNT11 glycosylates Notch to orchestrate cilia type and laterality.. Nature, (19): [PMID:24226769]
31. Chakrabarty, Broto and Parekh, Nita.. (2014) Identifying tandem Ankyrin repeats in protein structures.. BMC bioinformatics, (30): [PMID:25547411]
32. Pinnell, Nancy N and 14 more authors.. (2015) The PIAS-like Coactivator Zmiz1 Is a Direct and Selective Cofactor of Notch1 in T Cell Development and Leukemia.. Immunity, (17): [PMID:26522984]
33. Deatherage, Catherine L CL and 7 more authors.. (2017) Structural and biochemical differences between the Notch and the amyloid precursor protein transmembrane domains.. Science advances, [PMID:28439555]
34. Takeuchi, Hideyuki H and 6 more authors.. (2018) Two novel protein O-glucosyltransferases that modify sites distinct from POGLUT1 and affect Notch trafficking and signaling.. Proceedings of the National Academy of Sciences of the United States of America, (4): [PMID:30127001]

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所需的复溶浓度

NOTCH1 Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器