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Recombinant 14-3-3 gamma/YWHAG Antibody

Recombinant 14-3-3 gamma/YWHAG Antibody

级别和纯度:

0.25 mg/mL

应用:

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Rabbit IgG
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab086603-10μl	10μl	现货
Ab086603-50μl	50μL	现货
Ab086603-100μl	100μL	期货
Ab086603-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: YWHAG

14-3-3 gamma (1) Accession#:P61981 (1) Gene ID:7532 (1) YWHAG (1)

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基本信息

产品名称	Recombinant 14-3-3 gamma/YWHAG Antibody
别名	14-3-3γ抗体 \| 重组14-3-3γ/YWHAG抗体
英文别名	1433 gamma \| 14-3-3 gamma \| 14-3-3 protein gamma \| 14-3-3GAMMA \| KCIP-1 \| Protein kinase C inhibitor protein 1 \| tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gammapolypeptide \| YWHAG \| 14 3 3 gamma antibody \| 14 3 3 protein gamm
规格或纯度	重组, ExactAb™, Validated, KD, 0.25 mg/mL
宿主种属	兔(Rabbit)
特异性	YWHAG
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	A synthetic peptide derived from human 14-3-3 gamma (AA 135-186).
阳性对照	WB: HeLa, A549, A431 K562, Jurkat, NIH/3T3 and C6 cell lysates.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4
防腐剂	0.02% Sodium azide
浓度	0.25 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

YWHAG Tchem 14-3-3 蛋白质伽马(14-3-3 protein gamma) (0 活性数据)

点击查看靶蛋白信息： YWHAG

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Recombinant 14-3-3 gamma/YWHAG Antibody (Ab086603)

Recombinant 14-3-3 gamma/YWHAG Antibody (Ab086603) - Western Blot
All lanes: Recombinant 14-3-3 gamma/YWHAG Antibody (Ab086603) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 41.0 s

Western Blot: Recombinant 14-3-3 gamma/YWHAG Antibody (Ab086603)

应用

应用名称	稀释比例
WB	1/500-1/1000

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ24F0202977	分析证书	Ab086603
ZJ24F0202976	分析证书	Ab086603

技术文档和文章

蛋白质印迹的一抗选择指南

Categories: 实验方案（Protocols）

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重组抗体单克隆抗体多克隆抗体蛋白质印迹内参抗体一抗选择

Guidelines of Primary Antibodies for Western Blotting

Categories: 实验方案（Protocols）

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Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

Guidelines of Primary Antibodies for Western Blotting

Categories: 实验方案（Protocols）

探索主题:

Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

高分子量蛋白质印迹法

Categories: 实验方案（Protocols）

探索主题:

免疫印迹生物缓冲液蛋白质印迹高分子量蛋白大分子蛋白蛋白质电泳 WB抗体

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Protocol of Western Blotting For High Molecular Weights

Categories: 实验方案（Protocols）

探索主题:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

引用文献

1. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940]
2. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]
3. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
4. Stevers LM, Sijbesma E, Botta M, MacKintosh C, Obsil T, Landrieu I, Cau Y, Wilson AJ, Karawajczyk A, Eickhoff J, Davis J, Hann M, O'Mahony G, Doveston RG, Brunsveld L, Ottmann C.. (2018) Modulators of 14-3-3 Protein-Protein Interactions.. J Med Chem, 61 (9): (3755-3778). [PMID:28968506] [10.1021/acs.jmedchem.7b00574]
5. Autieri, M V MV and Carbone, C J CJ.. (1999) 14-3-3Gamma interacts with and is phosphorylated by multiple protein kinase C isoforms in PDGF-stimulated human vascular smooth muscle cells.. DNA and cell biology, [PMID:10433554]
6. Horie, M M, Suzuki, M M, Takahashi, E E and Tanigami, A A.. (1999) Cloning, expression, and chromosomal mapping of the human 14-3-3gamma gene (YWHAG) to 7q11.23.. Genomics, (1): [PMID:10486217]
7. Ganguly, S S and 13 more authors.. (2001) Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis.. Proceedings of the National Academy of Sciences of the United States of America, (3): [PMID:11427721]
8. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801]
9. Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10): [PMID:12853948]
10. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843]
11. Nagata-Ohashi, Kyoko K and 11 more authors.. (2004) A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia.. The Journal of cell biology, (24): [PMID:15159416]
12. Screaton, Robert A RA and 11 more authors.. (2004) The CREB coactivator TORC2 functions as a calcium- and cAMP-sensitive coincidence detector.. Cell, (1): [PMID:15454081]
13. Yoshida, Kiyotsugu K, Yamaguchi, Tomoko T, Natsume, Tohru T, Kufe, Donald D and Miki, Yoshio Y.. (2005) JNK phosphorylation of 14-3-3 proteins regulates nuclear targeting of c-Abl in the apoptotic response to DNA damage.. Nature cell biology, [PMID:15696159]
14. Jin, Yetao Y and 6 more authors.. (2006) 14-3-3gamma binds to MDMX that is phosphorylated by UV-activated Chk1, resulting in p53 activation.. The EMBO journal, (22): [PMID:16511572]
15. Angrand, Pierre-Olivier PO and 12 more authors.. (2006) Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.. Molecular & cellular proteomics : MCP, [PMID:16959763]
16. Yang, Xiaowen X and 8 more authors.. (2006) Structural basis for protein-protein interactions in the 14-3-3 protein family.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:17085597]
17. Jin, Yun-Hye YH and 6 more authors.. (2008) Sirt2 interacts with 14-3-3 beta/gamma and down-regulates the activity of p53.. Biochemical and biophysical research communications, (11): [PMID:18249187]
18. Brummer, Tilman T and 11 more authors.. (2008) Phosphorylation-dependent binding of 14-3-3 terminates signalling by the Gab2 docking protein.. The EMBO journal, (3): [PMID:19172738]
19. Kajiwara, Yuji Y, Buxbaum, Joseph D JD and Grice, Dorothy E DE.. (2009) SLITRK1 binds 14-3-3 and regulates neurite outgrowth in a phosphorylation-dependent manner.. Biological psychiatry, (15): [PMID:19640509]
20. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
21. Olsen, Jesper V JV and 11 more authors.. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.. Science signaling, (12): [PMID:20068231]
22. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
23. Greninger, Alexander L AL, Knudsen, Giselle M GM, Betegon, Miguel M, Burlingame, Alma L AL and DeRisi, Joseph L JL.. (2013) ACBD3 interaction with TBC1 domain 22 protein is differentially affected by enteroviral and kobuviral 3A protein binding.. mBio, (9): [PMID:23572552]
24. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569]
25. Yuasa, Keizo K and 5 more authors.. (2015) Suppression of death-associated protein kinase 2 by interaction with 14-3-3 proteins.. Biochemical and biophysical research communications, (14): [PMID:26047703]
26. Masters, Seth L SL and 31 more authors.. (2016) Familial autoinflammation with neutrophilic dermatosis reveals a regulatory mechanism of pyrin activation.. Science translational medicine, (30): [PMID:27030597]
27. Stevers, Loes M LM and 5 more authors.. (2017) Structural interface between LRRK2 and 14-3-3 protein.. The Biochemical journal, (23): [PMID:28202711]
28. Guella, Ilaria I and 17 more authors.. (2017) De Novo Mutations in YWHAG Cause Early-Onset Epilepsy.. American journal of human genetics, (3): [PMID:28777935]

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Recombinant 14-3-3 gamma/YWHAG Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器