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CD23 Antibody

    应用:
  • IHC
  • WB
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human), 小鼠(Mouse)
  • 亚型: Rabbit IgG
  • 偶联: Unconjugated
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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab006720-10μl
10μl 现货 Stock Image
Ab006720-50μl
50μL 现货 Stock Image
Ab006720-100μl
100μL 现货 Stock Image
Ab006720-1ml
1ml 期货 Stock Image

基本信息

产品名称 CD23 Antibody
别名 CD23 抗体
英文别名 BLAST-2 | C-type lectin domain family 4 member J | Fc-epsilon-RII | Immunoglobulin E-binding factor | Lymphocyte IgE receptor
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属 兔(Rabbit)
特异性 CD23
种属反应性 人(Human),小鼠(Mouse)
免疫原 Recombinant CD23 expressed in E.coli (AA 151-321)
阳性对照 WB: NIH/3T3, Raw264.7 and Raji cell lysates. IHC: Human Tonsil tissue, Human Amygdalitis tissue.
偶联 Unconjugated

产品属性

克隆类型 多克隆抗体
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Antigen affinity purified and protein A purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂 0.05% Proclin 300
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
分子类型 抗体

关联靶点(人)

FCER2 Tchem 低亲和力免疫球蛋白 ε Fc 受体(Low affinity immunoglobulin epsilon Fc receptor) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

CD23 Antibody (Ab006720) - Western Blot
All lanes: CD23 Antibody (Ab006720) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 36, 40 kDa
Exposure time: 22.9 s

CD23 Antibody (Ab006720) - IHC
DAB staining on IHC
Samples: Human Tonsil Tissue
Primary Ab: 10 µg/mL CD23 Antibody (Ab006720)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

CD23 Antibody (Ab006720) - IHC
DAB staining on IHC
Samples: Human Amygdalitis Tissue
Primary Ab: 20 μg/mL CD23 Antibody (Ab006720)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

应用

应用名称 稀释比例
IHC

5-20 µg/mL

WB

0.5-2 µg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ23F1001288 分析证书 Ab006720
ZJ23F1001289 分析证书 Ab006720
ZJ24F0911300 分析证书 Ab006720

引用文献

1. Yokota A, Kikutani H, Tanaka T, Sato R, Barsumian EL, Suemura M, Kishimoto T.  (1988)  Two species of human Fc epsilon receptor II (Fc epsilon RII/CD23): tissue-specific and IL-4-specific regulation of gene expression..  Cell,  55  (4): (611-8).  [PMID:2972386]
2. Rose, K K and 8 more authors..  (1992)  Partial characterization of natural and recombinant human soluble CD23..  The Biochemical journal,  (15): [PMID:1417742]
3. Pirron, U U, Schlunck, T T, Prinz, J C JC and Rieber, E P EP..  (1990)  IgE-dependent antigen focusing by human B lymphocytes is mediated by the low-affinity receptor for IgE..  European journal of immunology,  [PMID:2167225]
4. Kikutani, H H and 9 more authors..  (1986)  Molecular structure of human lymphocyte receptor for immunoglobulin E..  Cell,  (5): [PMID:2877743]
5. Ikuta, K K and 7 more authors..  (1987)  Human lymphocyte Fc receptor for IgE: sequence homology of its cloned cDNA with animal lectins..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:2949326]
6. Lüdin, C C and 8 more authors..  (1987)  Cloning and expression of the cDNA coding for a human lymphocyte IgE receptor..  The EMBO journal,  [PMID:3034567]
7. Vouldoukis, I I and 7 more authors..  (1995)  The killing of Leishmania major by human macrophages is mediated by nitric oxide induced after ligation of the Fc epsilon RII/CD23 surface antigen..  Proceedings of the National Academy of Sciences of the United States of America,  (15): [PMID:7544003]
8. Padlan, E A EA and Helm, B A BA..  (1993)  Modeling of the lectin-homology domains of the human and murine low-affinity Fc epsilon receptor (Fc epsilon RII/CD23)..  Receptor,  [PMID:8142907]
9. Bajorath, J J and Aruffo, A A..  (1996)  Structure-based modeling of the ligand binding domain of the human cell surface receptor CD23 and comparison of two independently derived molecular models..  Protein science : a publication of the Protein Society,  [PMID:8745401]
10. Grimwood, Jane J and 97 more authors..  (2004)  The DNA sequence and biology of human chromosome 19..  Nature,  (1): [PMID:15057824]
11. Hibbert, Richard G RG and 9 more authors..  (2005)  The structure of human CD23 and its interactions with IgE and CD21..  The Journal of experimental medicine,  (19): [PMID:16172256]
12. Wurzburg, Beth A BA, Tarchevskaya, Svetlana S SS and Jardetzky, Theodore S TS..  (2006)  Structural changes in the lectin domain of CD23, the low-affinity IgE receptor, upon calcium binding..  Structure (London, England : 1993),  [PMID:16765898]
13. Lemieux, George A GA and 9 more authors..  (2007)  The low affinity IgE receptor (CD23) is cleaved by the metalloproteinase ADAM10..  The Journal of biological chemistry,  (18): [PMID:17389606]
14. Pathan, Nuzhat I NI and 5 more authors..  (2008)  Mediation of apoptosis by and antitumor activity of lumiliximab in chronic lymphocytic leukemia cells and CD23+ lymphoma cell lines..  Blood,  (1): [PMID:18032710]
15. Ivaldi, Corinne C and 6 more authors..  (2012)  Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23..  PloS one,  [PMID:22615937]
16. Hersheson, Joshua J and 17 more authors..  (2013)  Mutations in the autoregulatory domain of β-tubulin 4a cause hereditary dystonia..  Annals of neurology,  [PMID:23424103]

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