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Recombinant Human EGF Protein, >95% SDS-PAGE, high purity

  • Expression System: Pichia pastoris
  • Accession #: P01133
  • Protein Tag: No tag
  • Bioactivity: Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL.
  • Endotoxin Concentration: <0.2 EU/μg
In stock
Item Number
rp145392
Grouped product items
SKU Size
Availability
Price Qty
rp145392-100μg
100μg
7
$69.90
rp145392-1mg
1mg
8
$399.90
rp145392-500μg
500μg
10
$299.90

GMP, ≥95% (SDS-PAGE), Active, Pichia pastoris, No tag, 971-1021aa

Basic Description

Product Name Recombinant Human EGF Protein, >95% SDS-PAGE, high purity
Synonyms beta-urogastrone | EGF | epidermal growth factor (beta-urogastrone) | epidermal growth factor | hEGF | HOMG4 | pro-epidermal growth factor | URG | Urogastrone | EGF
Grade ActiBioPure™, Animal Free, Bioactive, Carrier Free, GMP, High Performance
Specifications & Purity Carrier Free, GMP, Bioactive, ActiBioPure™, High Performance, Animal Free, ≥95%(SDS-PAGE)
Biochemical and Physiological Mechanisms EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR
Purity >95% SDS-PAGE
Bioactivity Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL.
Endotoxin Concentration <0.2 EU/μg
Expression System Pichia pastoris
Species Human
Amino Acids 971-1021 aa
Sequence NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWE
Protein Tag No tag
Protein Length Full length protein
Accession # P01133
Source Recombinant
Predicted molecular weight 6 kDa

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
Superclass Organic Polymers
Class Polypeptides
Subclass Not available
Intermediate Tree Nodes Not available
Direct Parent Polypeptides
Alternative Parents Peptides  Tyrosine and derivatives  Arginine and derivatives  Phenylalanine and derivatives  Histidine and derivatives  Glutamic acid and derivatives  Glutamine and derivatives  Asparagine and derivatives  Aspartic acid and derivatives  Isoleucine and derivatives  Leucine and derivatives  Methionine and derivatives  N-acyl-L-alpha-amino acids  Valine and derivatives  Proline and derivatives  Serine and derivatives  Cysteine and derivatives  Tryptamines and derivatives  Alpha amino acid amides  Alanine and derivatives  3-alkylindoles  Amphetamines and derivatives  N-acylpyrrolidines  Pyrrolidinecarboxamides  1-hydroxy-2-unsubstituted benzenoids  Substituted pyrroles  N-acyl amines  Tertiary carboxylic acid amides  Heteroaromatic compounds  Imidazoles  Guanidines  Secondary carboxylic acid amides  Primary carboxylic acid amides  Amino acids  Carboximidamides  Carboxylic acids  Dialkylthioethers  Sulfenyl compounds  Alkylthiols  Azacyclic compounds  Carbonyl compounds  Hydrocarbon derivatives  Imines  Monoalkylamines  Organic oxides  Primary alcohols  
Molecular Framework Aromatic heteropolycyclic compounds
Substituents Polypeptide - Alpha peptide - Tyrosine or derivatives - Arginine or derivatives - Phenylalanine or derivatives - Histidine or derivatives - Glutamic acid or derivatives - Glutamine or derivatives - Methionine or derivatives - Asparagine or derivatives - Aspartic acid or derivatives - Leucine or derivatives - Isoleucine or derivatives - N-acyl-l-alpha-amino acid - N-acyl-alpha amino acid or derivatives - N-acyl-alpha-amino acid - Valine or derivatives - Proline or derivatives - Triptan - Alpha-amino acid amide - Serine or derivatives - Cysteine or derivatives - Alanine or derivatives - 3-alkylindole - Amphetamine or derivatives - N-substituted-alpha-amino acid - Alpha-amino acid or derivatives - Indole - Indole or derivatives - N-acylpyrrolidine - Pyrrolidine carboxylic acid or derivatives - Pyrrolidine-2-carboxamide - 1-hydroxy-2-unsubstituted benzenoid - Phenol - Benzenoid - Fatty acyl - Monocyclic benzene moiety - Fatty amide - Substituted pyrrole - N-acyl-amine - Pyrrolidine - Pyrrole - Imidazole - Tertiary carboxylic acid amide - Heteroaromatic compound - Azole - Amino acid or derivatives - Carboxamide group - Guanidine - Secondary carboxylic acid amide - Primary carboxylic acid amide - Amino acid - Dialkylthioether - Carboximidamide - Azacycle - Organoheterocyclic compound - Carboxylic acid derivative - Carboxylic acid - Thioether - Sulfenyl compound - Alkylthiol - Hydrocarbon derivative - Primary amine - Organonitrogen compound - Carbonyl group - Organooxygen compound - Organosulfur compound - Primary alcohol - Organic nitrogen compound - Organic oxide - Imine - Alcohol - Primary aliphatic amine - Organic oxygen compound - Amine - Aromatic heteropolycyclic compound
Description This compound belongs to the class of organic compounds known as polypeptides. These are peptides containing ten or more amino acid residues.
External Descriptors Not available

Storage and Shipping

Shape Lyophilized
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 10mM PBS to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃. Further dilutions should be made in appropriate buffered solutions.
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8℃ short term (4 weeks). Store at -20℃ long term (24 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
CAS 62253-63-8

Images

Recombinant Human EGF Protein (rp145392)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9ng/mL.

Recombinant Human EGF Protein (rp145392)-SDS-PAGE
5μg/lane of Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 6.6 kDa.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ23F0400094 Certificate of Analysis Apr 25, 2023 rp145392
ZJ23F0400095 Certificate of Analysis Apr 25, 2023 rp145392
ZJ23F0400093 Certificate of Analysis Apr 19, 2023 rp145392

Alternative Products

Citations of This Product

1. Xiaoli Lu, Shangwen Sun, Na Li, Shuying Hu, Yuyao Pan, Lin Wang, Xuefeng Zhou, Hanbang Chen, Feimin Zhang.  (2024)  Janus sponge/electrospun fibre composite combined with EGF/bFGF/CHX promotes reconstruction in oral tissue regeneration.  COLLOIDS AND SURFACES B-BIOINTERFACES,  243  (114117). 

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