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Recombinant Human EGF Protein

  • Accession #: P01133
  • Bioactivity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.6 ng/mL.
  • Endotoxin Concentration: <1.0 EU/μg
In stock
Item Number
rp215069
Grouped product items
SKU Size
Availability
Price Qty
rp215069-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$14.90
rp215069-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$43.90
rp215069-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
rp215069-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$439.90

Carrier Free, ≥95% (SDS-PAGE), Active, E. coli, No tag, 971-1023 aa

Basic Description

Product Name Recombinant Human EGF Protein
Synonyms Beta urogastrone | beta-urogastrone | EGF | EGF_HUMAN | Epidermal growth factor | HOMG4 | OTTHUMP00000219721 | OTTHUMP00000219722 | Pro epidermal growth factor | URG | Urogastrone
Grade ActiBioPure™, Bioactive, Carrier Free, High Performance
Specifications & Purity Carrier Free, Bioactive, ActiBioPure™, High Performance, ≥95%(SDS-PAGE), See COA
Biochemical and Physiological Mechanisms EGF is the prototypic member of a family of growth factors that also includes amphiregulin, betacellulin, epigen, epiregulin, HB-EGF, neuregulins-1 through -6, and TGF-alpha. These proteins contain EGF-like domains with three intramolecular disulfide bond
Bioactivity Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.6 ng/mL.
Endotoxin Concentration <1.0 EU/μg
Amino Acids 971-1023 aa
Sequence SNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR​
Accession # P01133
Predicted molecular weight 6.3 kDa
SDS-PAGE 10.0 kDa, under reducing conditions; 10.0 & 11.4 kDa, under non-reducing conditions.

Storage and Shipping

Concentration See COA
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C for 1 year. Avoid freeze/thaw cycle.

Images

Recombinant Human EGF Protein (rp215069) - Bioactivity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.6 ng/mL.

Recombinant Human EGF Protein (rp215069) - SDS-PAGE
3 μg/lane of Recombinant Human EGF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 10.0 kDa under reducing conditions and 10.0 & 11.4 kDa under non-reducing conditions.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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