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Recombinant Human EGF Protein - Recombinant protein, ≥95% (SDS-PAGE), high purity, CAS No.62253-63-8
Expression System:
Pichia pastoris
Accession #:
P01133
Protein Tag:
No tag
Bioactivity:
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.
Endotoxin Concentration:
<1.0 EU/mg
GMP, ≥98% (SDS-PAGE), Active, Pichia pastoris, No tag, 971-1023 aa
Basic Description
Product Name
Recombinant Human EGF Protein - Recombinant protein, ≥95% (SDS-PAGE), high purity, CAS No.62253-63-8
Synonyms
UNII-TZK30RF92W | Human EGF | MG 111 | CCRIS 6735 | Nepidermin [INN] | NEPIDERMIN | Human epidermal growth factor | TZK30RF92W | beta-Urogastrone (human) | Epidermal growth factor (human) | AKOS040759488 | DTXSID80211314 | EGF
Grade
Animal Free, Bioactive, Carrier Free, GMP, High Performance, Low Endotoxin
Specifications & Purity
Animal Free, Carrier Free, GMP, Low Endotoxin, High performance, Bioactive grade, ≥98%(SDS-PAGE)
Biochemical and Physiological Mechanisms
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR
Purity
≥95% (SDS-PAGE)
Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.
Endotoxin Concentration
<1.0 EU/mg
Expression System
Pichia pastoris
Species
Human
Amino Acids
971-1023 aa
Sequence
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Protein Tag
No tag
Protein Length
Full length protein
Accession #
P01133
Source
Recombinant
Predicted molecular weight
6.5kD
SDS-PAGE
7.1 kDa, under reducing conditions.
Taxonomic Classification
Kingdom
Organic compounds
Superclass
Organic Polymers
Class
Polypeptides
Subclass
Not available
Intermediate Tree Nodes
Not available
Direct Parent
Polypeptides
Alternative Parents
Peptides Tyrosine and derivatives Arginine and derivatives Phenylalanine and derivatives Histidine and derivatives Glutamic acid and derivatives Glutamine and derivatives Asparagine and derivatives Aspartic acid and derivatives Isoleucine and derivatives Leucine and derivatives Methionine and derivatives N-acyl-L-alpha-amino acids Valine and derivatives Proline and derivatives Serine and derivatives Cysteine and derivatives Tryptamines and derivatives Alpha amino acid amides Alanine and derivatives 3-alkylindoles Amphetamines and derivatives N-acylpyrrolidines Pyrrolidinecarboxamides 1-hydroxy-2-unsubstituted benzenoids Substituted pyrroles N-acyl amines Tertiary carboxylic acid amides Heteroaromatic compounds Imidazoles Guanidines Secondary carboxylic acid amides Primary carboxylic acid amides Amino acids Carboximidamides Carboxylic acids Dialkylthioethers Sulfenyl compounds Alkylthiols Azacyclic compounds Carbonyl compounds Hydrocarbon derivatives Imines Monoalkylamines Organic oxides Primary alcohols
Molecular Framework
Aromatic heteropolycyclic compounds
Substituents
Polypeptide - Alpha peptide - Tyrosine or derivatives - Arginine or derivatives - Phenylalanine or derivatives - Histidine or derivatives - Glutamic acid or derivatives - Glutamine or derivatives - Methionine or derivatives - Asparagine or derivatives - Aspartic acid or derivatives - Leucine or derivatives - Isoleucine or derivatives - N-acyl-l-alpha-amino acid - N-acyl-alpha amino acid or derivatives - N-acyl-alpha-amino acid - Valine or derivatives - Proline or derivatives - Triptan - Alpha-amino acid amide - Serine or derivatives - Cysteine or derivatives - Alanine or derivatives - 3-alkylindole - Amphetamine or derivatives - N-substituted-alpha-amino acid - Alpha-amino acid or derivatives - Indole - Indole or derivatives - N-acylpyrrolidine - Pyrrolidine carboxylic acid or derivatives - Pyrrolidine-2-carboxamide - 1-hydroxy-2-unsubstituted benzenoid - Phenol - Benzenoid - Fatty acyl - Monocyclic benzene moiety - Fatty amide - Substituted pyrrole - N-acyl-amine - Pyrrolidine - Pyrrole - Imidazole - Tertiary carboxylic acid amide - Heteroaromatic compound - Azole - Amino acid or derivatives - Carboxamide group - Guanidine - Secondary carboxylic acid amide - Primary carboxylic acid amide - Amino acid - Dialkylthioether - Carboximidamide - Azacycle - Organoheterocyclic compound - Carboxylic acid derivative - Carboxylic acid - Thioether - Sulfenyl compound - Alkylthiol - Hydrocarbon derivative - Primary amine - Organonitrogen compound - Carbonyl group - Organooxygen compound - Organosulfur compound - Primary alcohol - Organic nitrogen compound - Organic oxide - Imine - Alcohol - Primary aliphatic amine - Organic oxygen compound - Amine - Aromatic heteropolycyclic compound
Description
This compound belongs to the class of organic compounds known as polypeptides. These are peptides containing ten or more amino acid residues.
External Descriptors
Not available
Data sources
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
Storage and Shipping
Shape
Lyophilized
Reconstitution
Reconstitute in sterile water to a concentration of 100-200μg/ml, and further dilute it in other solvents. Upon reconstitution, the product could be stored at 4 ℃ for 2-7 days and used up as soon as possible. For future use, should be stored at -20 ℃.
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ for 3 years. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
CAS
62253-63-8
Images
Protein Bioactivity: Recombinant Human EGF Protein (rp155969)
Recombinant Human EGF Protein (rp155969)-Protein Bioactivity
SDS-PAGE: Recombinant Human EGF Protein (rp155969)
Recombinant Human EGF Protein (rp155969)-SDS-PAGE
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
Citations of This Product
1.
Cao Mingbo, Ren Yupeng, Li Yuxuan, Deng Junfeng, Su Xiaorui, Tang Yongchang, Yuan Feng, Deng Haixia, Yang Gaoyuan, He Zhiwei, Liu Bo, Yao Zhicheng, Deng Meihai.
(2023)
Lnc-ZEB2-19 Inhibits the Progression and Lenvatinib Resistance of Hepatocellular Carcinoma by Attenuating the NF-κB Signaling Pathway through the TRA2A/RSPH14 Axis. International Journal of Biological Sciences,
19
(12):
(3678-3693).
2.
Maoze Guo, Yuqiu Wang, Bingbing Gao, Bingfang He.
(2021)
Shark Tooth-Inspired Microneedle Dressing for Intelligent Wound Management. ACS Nano,
15
(9):
(15316–15327).
3.
Xiaoli Lu, Shangwen Sun, Na Li, Shuying Hu, Yuyao Pan, Lin Wang, Xuefeng Zhou, Hanbang Chen, Feimin Zhang.
(2024)
Janus sponge/electrospun fibre composite combined with EGF/bFGF/CHX promotes reconstruction in oral tissue regeneration. COLLOIDS AND SURFACES B-BIOINTERFACES,
243
(114117).
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3 Citations
ZJ24F0607416