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RPA32/RPA2 Mouse mAb - Primary antibody, specific to RPA2, Mouse IgG1

COA COA
    Application:
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: 1591CT183.68.6
  • Conjugation: Unconjugated
In stock
Item Number
Ab125728
Grouped product items
SKU Size
Availability
 Price Qty
Ab125728-10μl
10μl
2
$66.90
Ab125728-50μl
50μl
1
$199.90
Ab125728-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$329.90
Ab125728-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,639.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (1591CT183.68.6); Mouse anti Human RPA32/RPA2 Antibody; WB, IHC; Unconjugated

View related series
Click to view target information: RPA2
Accession#:P15927 (2) Gene ID:6118 (2) RPA2 (2) RPA32 (2)

Basic Description

Product Name RPA32/RPA2 Mouse mAb - Primary antibody, specific to RPA2, Mouse IgG1
Synonyms REPA2 | Replication factor A protein 2 | replication protein A 32 kDa subunit | Replication protein A 34 kDa subunit | replication protein A2 (32kD) | replication protein A2, 32kDa | RF-A protein 2 | RP-A p32 | RP-A p34 | RPA2 | RPA32 | RPA34 | 60S acidic
Specifications & Purity ExactAb™, Validated, Carrier Free, High performance, 0.5 mg/mL
Host species Mouse
Specificity RPA2
Immunogen Recombinant protein of human RFA2 expressed in E.coli (AA 1-270)
Positive Control WB: HeLa, MCF7, Jurkat, Ramos and K562 cell lysates. IHC: Human tonsil tissue.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti human RPA32/RPA2 Antibody, Monoclonal (1591CT183.68.6), could be used for WB, IHC and so on.
Application:
WB: 1/4000
IHC：1/100~1/500
Protein Function:
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

RPA2 Tbio Replication protein A 32 kDa subunit (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

RPA32/RPA2 Mouse mAb (Ab125728) - Western Blot
All lanes: RPA32/RPA2 Mouse mAb (Ab125728) at 1/4000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 30 kDa
Observed band size: 31 kDa
Exposure time: 50.0 s

RPA32/RPA2 Mouse mAb (Ab125728) - IHC
RPA32/RPA2 Mouse mAb (Ab125728) staining RFA2 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Application

Application Dilution info
IHC

1/100-1/500
WB

1/4000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0102589 Certificate of Analysis Jan 25, 2024 Ab125728
ZJ24F0102588 Certificate of Analysis Jan 25, 2024 Ab125728

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