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Prostate Specific Antigen Antibody - Primary antibody, specific to Prostate Specific Antigen, Rabbit IgG

    Application:
  • IF/ICC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab123335
Grouped product items
SKU Size
Availability
Price Qty
Ab123335-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab123335-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$189.90
Ab123335-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab123335-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

pAb; Rabbit anti Human Prostate Specific Antigen Antibody; WB, IF/ICC; Unconjugated

Basic Description

Product Name Prostate Specific Antigen Antibody - Primary antibody, specific to Prostate Specific Antigen, Rabbit IgG
Synonyms antigen, prostate-specific antibody | APS antibody | Gamma seminoprotein antibody | Gamma-seminoprotein antibody | hK3 antibody | Kallikrein 3 antibody | Kallikrein related peptidase 3 antibody | Kallikrein-3 antibody | KLK 3 antibody | KLK2A1antibody | K
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Biochemical and Physiological Mechanisms Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Host species Rabbit
Specificity Prostate Specific Antigen
Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Prostate Specific Antigen.
Positive Control WB: LNCaP cell lysate. IF/ICC: MCF7 cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human Prostate Specific Antigen Antibody, Polyclonal, could be used for WB, IF/ICC and so on.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

KLK3 Tclin Prostate-specific antigen (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Prostate Specific Antigen Antibody (Ab123335) - Western Blot
All lanes: Prostate Specific Antigen Antibody (Ab123335) at 1/2000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 29, 32 kDa
Prostate Specific Antigen Antibody (Ab123335) recognizes different isoforms of Prostate Specific Antigen.
Exposure time: 20.0 s

Prostate Specific Antigen Antibody (Ab123335) - IF/ICC
Immunofluorescent analysis of Kallikrein 3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X - 100 in TBS for 5 - 10 minutes and blocked with 3% BSA - PBS for 30 minutes at room temperature. Cells were probed with Prostate Specific Antigen Antibody (Ab123335) in 3% BSA - PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488 - conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Application

Application Dilution info
WB

1/500-1/2000

IF/ICC

1/50-1/200

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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