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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab123335-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$59.90
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Ab123335-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$189.90
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Ab123335-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
|
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Ab123335-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
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pAb; Rabbit anti Human Prostate Specific Antigen Antibody; WB, IF/ICC; Unconjugated
| Product Name | Prostate Specific Antigen Antibody - Primary antibody, specific to Prostate Specific Antigen, Rabbit IgG |
|---|---|
| Synonyms | antigen, prostate-specific antibody | APS antibody | Gamma seminoprotein antibody | Gamma-seminoprotein antibody | hK3 antibody | Kallikrein 3 antibody | Kallikrein related peptidase 3 antibody | Kallikrein-3 antibody | KLK 3 antibody | KLK2A1antibody | K |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. |
| Host species | Rabbit |
| Specificity | Prostate Specific Antigen |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Prostate Specific Antigen. |
| Positive Control | WB: LNCaP cell lysate. IF/ICC: MCF7 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human Prostate Specific Antigen Antibody, Polyclonal, could be used for WB, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Prostate Specific Antigen Antibody (Ab123335) - Western Blot
All lanes: Prostate Specific Antigen Antibody (Ab123335) at 1/2000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 29, 32 kDa
Prostate Specific Antigen Antibody (Ab123335) recognizes different isoforms of Prostate Specific Antigen.
Exposure time: 20.0 s
Prostate Specific Antigen Antibody (Ab123335) - IF/ICC
Immunofluorescent analysis of Kallikrein 3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X - 100 in TBS for 5 - 10 minutes and blocked with 3% BSA - PBS for 30 minutes at room temperature. Cells were probed with Prostate Specific Antigen Antibody (Ab123335) in 3% BSA - PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488 - conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| IF/ICC |
1/50-1/200
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