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PAK4 Mouse mAb - Primary antibody, specific to PAK4, Mouse IgG1

    Application:
  • IHC
  • IP
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: K1E023_12G11
  • Conjugation: Unconjugated
In stock
Item Number
Ab120121
Grouped product items
SKU Size
Availability
Price Qty
Ab120121-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab120121-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab120121-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab120121-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

mAb (K1E023_12G11); Mouse anti Human PAK4 Antibody; WB, IHC, IP; Unconjugated

Basic Description

Product Name PAK4 Mouse mAb - Primary antibody, specific to PAK4, Mouse IgG1
Synonyms EC 2.7.11.1 | p21-activated kinase 4 | PAK-4 | KIAA1142 antibody | p21 activated kinase 4 antibody | p21 Cdc42/Rac1-actiated kinase 4 antibody | P21 protein (Cdc42/Rac) activated kinase 4 antibody | p21(CDKN1A) activated kinase 4 antibody | p21-activated
Specifications & Purity Carrier Free, ExactAb™, Validated, 1.0 mg/mL
Biochemical and Physiological Mechanisms Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/de
Host species Mouse
Specificity PAK4
Immunogen Recombinant human PAK4 (AA 101-220).
Positive Control WB: HEK293, Jurkat, HeLa, MCF7, LNCaP, NIH/3T3 and C6 cell lysates. IHC: Human prostate tissue. IP: HEK293 lysates.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human PAK4 Antibody, Monoclonal (K1E023_12G11), could be used for WB, IHC, IP and so on.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -80°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

PAK4 Tchem Serine/threonine-protein kinase PAK 4 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

PAK4 Mouse mAb (Ab120121) - Western Blot
All lanes: PAK4 Mouse mAb (Ab120121) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 74, 75 kDa
PAK4 can function as an antiapoptotic protein (PMID:14517283). And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).
Exposure time: 2.9 s

PAK4 Mouse mAb (Ab120121) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of PAK4 Mouse mAb (Ab120121) with HEK293 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. PAK4 Mouse mAb (Ab120121) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Light chain specific) was used as the secondary antibody.
Lane 1: HEK293 lysate
Lane 2: PAK4 immunoprecipitated from HEK293 lysate by PAK4 Mouse mAb (Ab120121)
Lane 3: The same as Lane 2 but mouse IgG was used as IgG isotype control antibody
Result: PAK4 Mouse mAb (Ab120121) can immunoprecipitate PAK4.

PAK4 Mouse mAb (Ab120121) - IHC
IHC analysis of prostate tissue by PAK4 Mouse mAb (Ab120121).
IHC was performed using sections of the formalin-fixed paraffin-embedded prostate tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with PAK4 Mouse mAb (Ab120121) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Glandular cells are positively stained at the cytoplasm.

Application

Application Dilution info
WB

1/1000

IP

1/100

IHC

1/200

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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